Fig 2: Autophagy degradation disorder after Sidt2 deletion in mouse kidney cells.A LC3-II and P62 expression by MPC5 cells incubated for 16 h with 0, 10, 25, 50, 100, or 200 µM CQ shown by western blot; B LC3-II statistical chart of MPC5 cells; C P62 statistical chart of MPC5 cells; D SV40 MES 13 cell incubation for 16 h with 0, 10, 25, 50, 100, or 200 µM CQ. Western blot shows LC3-II and P62 expression; E LC3-II statistical chart of SV40 MES 13 cells; F SV40 MES 13 cell P62 statistical chart; G determination whether CQ induces the expression of key autophagy proteins before and after Sidt2 knockout in MPC5 cells; H statistical chart of (G); I changes in the autophagy flux in MPC5 cells on Sidt2 knockout (LC3-II + CQ/ß-Actin)/(LC3-II - CQ/ß-Actin) (ref. [27]); J determination whether chloroquine induces the expression of key autophagy proteins before and after Sidt2 knockout in SV40 MES 13 cells; K statistical chart of (J); L changes of the autophagy flux in SV40 MES 13 cells on Sidt2 knockout (LC3-II + CQ/ß-Actin)/(LC3-II - CQ/ß-Actin) (ref. [27]). *P < 0.05, **P < 0.01, ***P < 0.001.

Fig 3: Sidt2 deletion disrupts the autophagy pathway.A Detection of WT and Sidt2-/- cell autophagy-related protein expression levels by western blot; B statistical charts of the western blot test results; C P62 mRNA expression level in kidney tissues of WT and Sidt2-/- mice; D expression levels of MPC5 cell autophagy pathway proteins before and after Sidt2 knockout; E statistical graph of (D) chart; F expression levels of autophagy pathway proteins in SV40 MES 13 cells before and after Sidt2 knockout; G statistical chart of the (F) chart; H P62 immunofluorescence in MPC5 and SV40 MES 13 cells before and after Sidt2 knockout; I statistical charts of the immunofluorescence results; J P62 mRNA expression levels in MPC5 and SV40 MES 13 cells before and after Sidt2 knockout. *P < 0.05, **P < 0.01, ***P < 0.001.

Fig 4: Autophagy lysosomal degradation pathway is blocked after Sidt2 deletion.A Ad-mCherry-GFP-LC3B application to detect autophagy flow in MPC5 and SV40 MES 13 cells before and after Sidt2 knockout; B statistical chart of MPC5 cell autophagosome and autophagolysosome detection results; C statistical chart of SV40 MES 13 cell autophagosome and autophagolysosome detection results. *P < 0.05.

Fig 5: Upstream activator of autophagy cannot improve P62 accumulation induced by Sidt2 knockout.A Changes of key autophagy proteins in MPC5 cells on adding rapamycin; B statistical chart of (A); C P62 mRNA expression in MPC5 cells before and after adding rapamycin; D changes of key autophagy proteins in SV40 MES 13 cells on adding rapamycin; E statistical chart of (D); F P62 mRNA expression in SV40 MES 13 cells on adding rapamycin; *P < 0.05, **P < 0.01, ***P < 0.001.