Fig 2: Gene Set Enrichment Analysis (GSEA)(A) GSEA of Cd8b1.(B) GSEA of Pdcd1.(C) GSEA of Klrd1.(D) GSEA of Klrk1.(E) GSEA of Cd3g.(F) GSEA of Cd74.(G) GSEA of Cd3d. Different color curves represented different KEGG pathways, the Y axis represented a degree of enrichment, and the vertical line represented the genes enriched in the pathway.

Fig 3: TRIM13 interacted with CD3D and regulated its ubiquitination(A) CO-IP.(B) GST pull-down.(C and D) Detection of transfection efficiency by RT-qPCR and western blot.(E) Detected the expression of TRIM13 and CD3D by western blot.(F) Ubiquitin level analysis. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (n = 3). Data are represented as mean ± SD.

Fig 4: Knockdown of CD3D could relieve OGD-induced cardiomyocyte apoptosis(A and B) Detection of transfection efficiency by RT-qPCR and western blot.(C) CCK-8 assay.(D) Detected the levels of LDH and cTnT by ELISA.(E) Detected the levels of IL-1β, IL-6, and TNF-α by ELISA.(F) Detected the expression of cTnT by IF (Scale bars, 20 μm).(G) Detection of cell apoptosis rate by flow cytometry.(H) Detected the expression of cleaved-caspase-3, Bax and Bcl-2 by western blot. ∗∗p < 0.01, ∗∗∗p < 0.001, vs. NC group. #p < 0.05, ##p < 0.01, ###p < 0.001, vs. OGD group (n = 3). Data are represented as mean ± SD.

Fig 5: Knockdown of CD3D could alleviate the progression of MI mice(A) Left ventricular FS and EF of the MI mouse.(B) HE staining (Scale bars, 50 μm).(C) TTC staining.(D) Detected the expression of TRIM13 and CD3D by western blot.(E) Detected the levels of LDH and cTnT by ELISA.(F) Detected the levels of IL-1β, IL-6, and TNF-αby ELISA.(G) Detected the expression of cTnT by IF (Scale bars, 20 μm).(H) TUNEL staining (Scale bars, 20 μm).(I) Detected the expression of cleaved-caspase-3, Bax and Bcl-2 by western blot. ∗∗p < 0.01, ∗∗∗p < 0.001, vs. Sham group. #p < 0.05, ##p < 0.01, vs. MI group (n = 6). Data are represented as mean ± SD.