Histone H2AX Antibody

Product Specs
ItemHistone H2AX Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberNB100-383
ImmunogenThe immunogen recognized by this antibody maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014)
Concentration1.0 mg/ml
Conjugate/TagUnconjugated
FormatTris-Citrate/Phosphate (pH 7.0 - 8.0)
ApplicationsIF/IHC,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry/ Immunofluorescence
ReactivityHuman,Mouse
Quantity100 ul
TargetHistone H2AX
HostRabbit
IsotypeIgG
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaDNA Repair, Mitotic Regulators
NCBI Full Gene NameH2A.X variant histone
NCBI Gene AliasesH2AFX, H2A/X, H2A.X
Antibody TypePrimary Antibodies
StorageStore at 4C. Do not freeze

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: This is specific for H2AX. It is not known if this also recognizes the phosphorylated form of H2AX.

Sizes Available: 100 ul

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Questions and Answers

Can this antibody be used for studying the role of H2AX in neuronal cell death?

Yes, the Histone H2AX Antibody has been utilized in studies examining the interaction between apoptosis inducing factor and H2AX in the context of neuronal cell death. For instance, in a study involving traumatic brain injury, the antibody was used to assess H2AX activation and its role in AIF-mediated cell death, demonstrating its relevance in neuroprotection research ().

What applications has this antibody been shown to work in?

The Histone H2AX Antibody has been shown to work in various applications including Western Blotting, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Its effectiveness in these applications has been documented in scientific literature, particularly in studies related to DNA repair and cell death mechanisms.

Is this antibody specific for human and mouse H2AX?

Yes, the Histone H2AX Antibody is specific for H2AX and has been demonstrated to react with both human and mouse samples, making it suitable for comparative studies across these species.

How has this antibody been utilized in immunohistochemistry studies?

The Histone H2AX Antibody has been employed in immunohistochemistry to analyze H2AX expression in mouse models following traumatic brain injury. In such studies, it has been used to visualize H2AX activation in tissue sections, contributing to the understanding of its role in cellular responses to injury ().

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint DNA molecule resolutionMolecular cellFebruary 15, 2024Tsukada K, Jones SE, Bannister J, Durin MA, Vendrell I, Fawkes M, Fischer R, Kessler BM, Chapman JR, Blackford AN.

(2) Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered regioneLifeApril 13, 2022Balasubramanian S, Andreani M, Andrade JG, Saha T, Sundaravinayagam D, Garzón J, Zhang W, Popp O, Hiraga SI, Rahjouei A, et al.(S139)(mouse monoclonal),Millipore,05-636,WB (1:1000) Antibody,H2AX(rabbit polyclonal),Novus Biologicals,NB100-383,WB (1:1000) Antibody,HRP-goat anti-rabbit heavy chain(goat polyclonal),Jackson ImmunoResearch,111-035-008,"WB

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(4) MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during MitosisMolecular cellMay 2, 2019Leimbacher PA, Jones SE, Shorrocks AK, de Marco Zompit M, Day M, Blaauwendraad J, Bundschuh D, Bonham S, Fischer R, Fink D, et al.(11814460001, Roche, 1/5000), GST (sc-138, Santa Cruz Biotechnology, 1/500), H2A (01-146, Merck, 1/2500), H2AX (NB100-383, Novus Biologicals, 1/5000), γH2AX (05-636, Merck, 1/1000), H3 (ab1791, Abcam, 1/30,000), H3-pS10 (ab14955,

(5) Combined inhibition of cell death induced by apoptosis inducing factor and caspases provides additive neuroprotection in experimental traumatic brain injuryNeurobiology of diseaseJune 1, 2012Piao CS, Loane DJ, Stoica BA, Li S, Hanscom M, Cabatbat R, Blomgren K, Faden AI.Biomol GmbH, Germany), AIF (1:1000, cat. no. 4642 Millipore, Billerica, MA), H2AX (1:1000, cat. no. NB100–383 Novus, Littleton, CO), CypA (1:1000, cat. no. ab41684, Abcam, Cambridge, MA). Beta-actin (1:5000, Sigma-Aldrich,

(6) Critical role of monoubiquitination of histone H2AX protein in histone H2AX phosphorylation and DNA damage responseThe Journal of biological chemistrySeptember 2, 2011Wu CY, Kang HY, Yang WL, Wu J, Jeong YS, Wang J, Chan CH, Lee SW, Zhang X, Lamothe B, et al.

(7) Combination of nutlin-3 and VX-680 selectively targets p53 mutant cells with reversible effects on cells expressing wild-type p53Cell death and differentiationSeptember 1, 2010Cheok CF, Kua N, Kaldis P, Lane DP.

(8) AIF promotes chromatinolysis and caspase-independent programmed necrosis by interacting with histone H2AXThe EMBO journalMay 5, 2010Artus C, Boujrad H, Bouharrour A, Brunelle MN, Hoos S, Yuste VJ, Lenormand P, Rousselle JC, Namane A, England P, et al.from control or MNNG-treated MEFs was subjected to immunoprecipitation with 2 mg of anti-H2AX antibody (Novus, NB100-383). H2AX/ MEFs were transfected as above with pcDNA3.1-H2AX, pcDNA3.1H2AXS139A, or pcDNA3.1 empty vector.

(9) WRN is required for ATM activation and the S-phase checkpoint in response to interstrand cross-link-induced DNA double-strand breaksMolecular biology of the cellSeptember 1, 2008Cheng WH, Muftic D, Muftuoglu M, Dawut L, Morris C, Helleday T, Shiloh Y, Bohr VA.

(10) Examining the link between chromosomal instability and aneuploidy in human cellsThe Journal of cell biologyFebruary 25, 2008Thompson SL, Compton DA.

(11) Developmental pluripotency-associated 4 (DPPA4) localized in active chromatin inhibits mouse embryonic stem cell differentiation into a primitive ectoderm lineageThe Journal of biological chemistryNovember 9, 2007Masaki H, Nishida T, Kitajima S, Asahina K, Teraoka H.

(12) Cross-species difference in telomeric function of tankyrase 1Cancer scienceJune 1, 2007Muramatsu Y, Ohishi T, Sakamoto M, Tsuruo T, Seimiya H.

(13) Senescence of human fibroblasts after psoralen photoactivation is mediated by ATR kinase and persistent DNA damage foci at telomeresMolecular biology of the cellApril 1, 2006Hovest MG, Brüggenolte N, Hosseini KS, Krieg T, Herrmann G.

(14) The telomerase reverse transcriptase regulates chromatin state and DNA damage responsesProceedings of the National Academy of Sciences of the United States of AmericaJune 7, 2005Masutomi K, Possemato R, Wong JM, Currier JL, Tothova Z, Manola JB, Ganesan S, Lansdorp PM, Collins K, Hahn WC.

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