Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb 9624L from Cell Signaling Technology

Product Specs
ItemPhospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb
CompanyCell Signaling Technology
Price $947.00 Inquire
Catalog Number9624L
Quantity300 µl
ApplicationsW, IP, E-P
ReactivityAll
HostRabbit
ClonalityMonoclonal
Research AreaMotif Antibodies
ModificationsPKA (RRXt) substrate motif phosphate
IsotypeIgG
Clone100G7E

Questions and Answers

Can this antibody be used to study PKA signaling in different cell types?

Yes, the Phospho-PKA Substrate (RRXS*/T*) antibody has been shown to be effective in various applications, including Western blotting and immunoprecipitation, across multiple species such as human, mouse, and zebrafish, as well as in different cell types. It has been utilized in studies to investigate PKA signaling pathways in various contexts, including cancer and cellular differentiation.

How has this antibody been applied in recent research?

The antibody has been cited in numerous studies, including research on microtubule acetylation dynamics where it was used to assess the phosphorylation state of substrates in response to PKA activity (). Additionally, it has been employed to investigate the role of PKA in regulating endothelial barrier function and stress fiber formation ().

Is this antibody specific for phosphorylated substrates, and how does it perform in detecting PKA activity?

The Phospho-PKA Substrate antibody specifically detects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 and -2 positions, making it a valuable tool for identifying PKA substrates. It has been shown to work effectively in various assays, including Western blotting, to monitor PKA activity and substrate phosphorylation levels ().

What experimental techniques can I use this antibody with?

This antibody is suitable for use in Western blotting, ELISA, and immunoprecipitation. It has been successfully utilized in studies to analyze phosphorylation states in various experimental setups, including those involving kinase signaling pathways and cellular responses to treatments (; ).

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Bile Canalicular Bitter Taste Receptors Inhibit β-Adrenergic Receptor-Induced Lipolysis in Steatotic HepatocytesInt J Mol SciApril 2, 2026Yan-Bo Xue, Shi-Meng Gong, Yuan-Yuan Peng, Defu Yu, Ruhong Zhou, Liquan Huangdiluted in the blocking buffer (anti-phospho-PKA substrate antibody, Cell Signaling, Danvers, MA, USA, 9624; anti-phosphor-HSL antibody, Cell Signaling 4139; anti-HSL antibody, Cell Signaling 4107; anti-GAPDH

(2) CaSR regulates SLC26A6 expression via the PKA-FOXO4 signaling axis to promote experimental calcium oxalate kidney stone formation in ratsFrontiers in Cell and Developmental BiologyMarch 20, 2026Lifeng Gan, Wei Li, Peiyue Luo, Fangtao Zhang, Haidong Zhong, Minggui Xie, Yiran Lu, Biao Qian, Liying Zhengpurchased from Gibco Company. Rabbit polyclonal antibodies against CaSR (73303S) and Phospho-PKA Substrate (9624S) were purchased from Cell Signaling Technology Company. Rabbit polyclonal antibody against p-FOXO4 (Thr451)

(3) Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fateNucleic Acids ResMarch 19, 2026Patrizia Maria Christiane Nothnagel, Paul-Arthur Meslin, Jonas Aakre Wik, Damien Dufour, Yunna Erika Strøm, Magnar Bjørås, Jorrit Martijn Enserink, Bjørn Steen Skålhegg, Nolwenn Briand, Anthony Mathelier, et al.and western blot. The following antibodies were used: UCP1, abcam, ab209483; PKA phospho-substrates, Cell Signaling, 9624; p-CREB/p-ATF1, Cell Signaling, 9198; p38 MAPK, Cell Signaling, 9212; p-p38 MAPK, Cell Signaling, 9211;

(4) Glycogen phosphorylase L confers metabolic flexibility in neutrophils to fight fungal infections in nutrient-deprived tissuesCell Host MicrobeMarch 18, 2026Wonseok Choi, De-Dong Li, Colin T. McLaughlin, Doureradjou Peroumal, Kiyoshi P. Shiomitsu, Gillian A. Moschetta, Hossein Rahimi, Shuxia Wang, Kiaan Biswas, Danielle Xie, et al.4782RRID: AB_2170170 Anti-phospho PKA substrate antibody (Clone 100G7E),Cell Signaling Technology,Cat# 9624 RRID: AB_331817 Anti-phospho S15-PYGL antibody Clone EPR20881-72),Abcam,Cat#ab227043 RRID: AB_3674858 Anti-PYGL

(5) Anti-Inflammatory Potential of Novel Tethered Agonists of the Adhesion G Protein-Coupled Receptor F5Int J Mol SciMarch 13, 2026Artur Wnorowski, Diana Pietrzak-Mitura, Akanksha Mudgal, Lorenzo Scrofani, Magdalena Strachowska, Piotr Draczkowski, Krzysztof Jóźwiak, Jakub Fichna, Damian Jacenik(#2331), teEF2 (#2332), pAKT (#4060), tAKT (#4691), pERK1/2 (#4370), tERK1/2 (#4695), PKA p-substrates (#9624), PKC p-substrates (#6967), or AMPK p-substates (#5759) overnight at 4 °C. For each sample, a separate

(6) Mitochondrial preservation through the PKA–DRP1 axis empowers natural killer cells to resist acidic stress and retain cytotoxicityCell Communication and SignalingMarch 6, 2026Eunchong Maeng, Ki Seo Ryu, A Reum Je, Minwook Lee, Hail Park, Seung Hee Choi, Kyung-Soon Parkincubated overnight at 4 °C with the following primary antibodies: Phospho-PKA Substrate (RRXS*/T*) (#9624, Cell Signaling), DRP1 (#5391, Cell Signaling), phospho-Ser637 (S637) DRP1 (PA5-37534, Thermo Fisher

(7) ANKRD53 is downregulated in human obesity and coordinates lipolysis with mitochondrial oxidative metabolism in adipocytesMolecular MetabolismMarch 1, 2026Yingying Su, Xiaoya Li, Yikai Wang, Xuhong Lu, Yafen Ye, Jingjing Sun, Tianwen Liu, Jinghao Cai, Xiaojing Ma, Ying Yang, et al.(Cell Signaling Technology, 45804), HSL (Cell Signaling Technology, 4107), Phospho-PKA Substrate (Cell Signaling Technology, 9624), and Histone H3 (Cell Signaling Technology, 4499). After washing, membranes were incubated for 1 h

(8) White-to-brown adipose switching promotes bladder cancer progressionNeoplasia (New York, N.Y.)March 1, 2026Gao M, Li C, Li W, Xie J, Long J, Luo M, Hu J, Lai C, Lan T, Zhu D, et al.anti-PTHR (1:1000, 29115-1-AP, Proteintech, China), anti-Phospho-PKA Substrate (RRXS*/T*) (1:1000, 9624, CST, USA), anti-pHSL (Ser660) (1:1000, AF8026, Affinity, China), anti-HSL (1:1000, AF6403, Affinity,

(9) Partial truncation of the C-terminal domain of PTCH1 in cancer promotes tumourigenesis by non-canonical activation of a GLI-PI3K loopOncogeneFebruary 26, 2026Begoña Caballero-Ruiz, Rosa Bordone, Sonia Coni, Danai S. Gkotsi, Eva Gonzalez, Gianluca Canettieri, Natalia A. Riobo-Del GaldoGli1 (Cell Signaling Technology #2643, WB 1:1,000), Phospho-PKA substrate (Cell Signaling Technology #9624, WB 1:1,000), GAPDH-HRP (Proteintech #HRP-60004, WB 1:3,000), Vinculin (Santa Cruz #sc-73614, WB 1:10,000),

(10) Thromboxane signalling links immune activation to enhanced glucose uptake in skeletal muscleDiabetologiaFebruary 20, 2026Ahmed M. Abdelmoez, Maxence Jollet, Xue Yu, David Rizo-Roca, Alesandra A. Marica, Joaquin Ortiz de Zevallos, Lucile Dollet, Melissa L. Borg, Marie Björnholm, Antonio Checa, et al.Primary antibodies were as follows: phospho-protein kinase A (PKA) substrates (1:1000, Cell Signaling 9624), phospho-filamin A (Ser2152, 1:1000, Cell Signaling 4761), phospho-cofilin (Ser3, 1:1000, Cell Signaling

(11) Lysine malonylation regulates human sperm motilityCommun BiolFebruary 9, 2026Yimin Cheng, Yan Tian, Houyang Chen, Shenglin Peng, Ying Chen, Zhen Peng, Tao Luopurchased from Abcam Limited (Cambridge, UK). The phospho-PKA substrate (RRXS*/T*) rabbit antibody (9624S) was procured from Cell Signaling Technology, Inc. (Danvers, MA, USA). Anti-PDK1 antibody (Ab-241) was

(12) Protein kinase C epsilon deletion in AgRP neurons modulates hypothalamic glucose sensing and improves glucose tolerance in miceMolecular MetabolismFebruary 1, 2026Amanda E. Brandon, Chenxu Yan, Xuan Zhang, Chi Kin Ip, Zhongmin Gao, Nicola J. Lee, Oana C. Marian, Alex Perez, Anthony S. Don, Herbert Herzog, et al.Cell Signalling Technology #9131), total STAT3 (Becton Dickinson, 610189), phospho-PKA substrate (Cell Signaling #9624), phospho-Ser133 CREB (Cell Signalling Technology #9198), phospho-Ser563 hormone sensitive lipase (HSL,

(13) Protein kinase a regulates cyclooxygenase-2 expression through the RNA-binding proteins HuR and TTPJournal of Biological ChemistryFebruary 1, 2026Sendi Rafael Adame-Garcia, Thomas S. Hoang, Pham Thuy Thien Vo, Valeria Burghi, Rodolfo Daniel Cervantes-Villagrana, Lennis Beatriz Orduña-Castillo, Dana J. Ramms, JoAnn Trejo, J. Silvio GutkindCα (#5842), GFP (#2956), GST (#2622), phospho-CREB S133 (#9198), CREB (#4820), and PKA substrates (#9624) were purchased from Cell Signaling Technology. Antibody for HuR (#39–0600) was purchased from Invitrogen

(14) KLF7 induced ADRB3-dependent IL-6 production in brown adipocytes during stressJournal of Lipid ResearchFebruary 1, 2026Maodi Liang, Meixiu Zhang, Yanting Hou, Fangyuan Yuan, Huizi Zhang, Mengyuan Zhao, Lili Xu, Qin Liu, Yurui Su, Xiaolong Chu, et al.primary antibodies against KLF7 (Abcam, ab197690), IL-6 (Abcam, ab290735), phospho-PKA substrate (CST, 9624), p-CREB (Proteintech, 28792-1-AP, China), CREB (Proteintech, 12208-1-AP, China), β-Tubulin (ZSGB-BIO,

(15) Structural dynamics of mixed-subunit CaMKIIα/β heterododecamers filmed by high-speed AFMNat CommunDecember 24, 2025Keisuke Matsushima, Takashi Sumikama, Taisei Suzuki, Mizuho Ito, Yutaro Nagasawa, Ayumi Sumino, Holger Flechsig, Tomoki Ogoshi, Kenichi Umeda, Noriyuki Kodera, et al.anti-phospho-CaMKII (Thr306) (p1005-306; PhosphoSolutions), anti-phospho-PKA substrate (RRXS*/T*) (#9624; Cell Signaling Technology), anti-His-tag (27E8; Cell Signaling Technology), anti-pan-CaMKII (D11A10;

(16) Extracellular Vesicles Released From Prostate Cancer Cells Confer Pro-Tumor Properties to Adipocytes by Stimulating LipolysisBiofactorsDecember 11, 2025Gaia Giannitti, Kinga Kamińska, Sara Marchesi, Riccardo Garavaglia, Ivan Preosto, Małgorzata Grzesiak, Fabrizio FontanaFor Western blot analyses, the following primary antibodies were used: anti‐ALIX (2171), anti‐p‐PKA substrate (9624), anti‐HSL (4107), anti‐p‐HSL (45804), anti‐ATGL (2439), anti‐Akt (2938), anti‐p‐Akt (9271), and anti‐GAPDH

(17) Inhibition of adipocyte lipolysis by vaspin impairs thermoregulation in vivoNat CommunDecember 10, 2025Inka Rapöhn, Helen Broghammer, Anne Hoffmann, Kevin Möhlis, Anna Moormann, Isabell Kaczmarek, Doreen Thor, Henning Großkopf, Laura Krieg, Isabel Karkossa, et al.primary antibodies were used: from Cell Signaling Technologies: phospho-PKA substrates (RRXS*/T*, #9624), HSL (#4107), phospho-HSL (Ser660, #4126), p38 MAPK (#9212), phospho-p38 MAPK (Thr180/Tyr182, #4511),

(18) GLP-1R associates with VAPB and SPHKAP at ERMCSs to regulate β-cell mitochondrial remodelling and functionNat CommunDecember 10, 2025Gregory Austin, Affiong I. Oqua, Liliane El Eid, Mingli Zhu, Yusman Manchanda, Priyanka Peres, Helena Coyle, Yelyzaveta Poliakova, Zhanna Balkhiyarova, Karim Bouzakri, et al.anti-GFP (Proteintech, 50430-2-AP, 1:2000); rabbit anti-phospho-PKA Substrate (RRXS*/T*) (Cell Signaling, 9624, 1:1000); rabbit anti-Drp1 (D6C7) (Cell Signaling, 8570S, 1:1000); rabbit anti-phospho-Drp1 Ser616 (D9A1)

(19) TOR and heat shock response pathways regulate peroxisome biogenesis during proteotoxic stressNature CommunicationsNovember 28, 2025Nandini Shukla, Maxwell L. Neal, Jean-Claude Farré, Fred D. Mast, Rajasri Sarkar, Linh Truong, Theresa Simon, Leslie R. Miller, John D. Aitchison, Suresh Subramani(#4511, 1:2000); Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb, Cell Signaling Technology (#9624, 1:2000); Anti-ScActin Rabbit pAb20, Gift from Michael Yaffe (1:5000); Goat Anti-Rabbit IgG (H + L)-HRP Conjugate

(20) Exercise attenuates hepatic ectopic lipid deposition in high fat-diet rats via PKA-mediated phosphorylation of Perilipin5Scientific ReportsNovember 27, 2025Shangyan Dong, Yang Yang, Wei Li, Lifeng Wang, Ting LiChina), Perilipin5 (26951-1-AP, Proteintech, Wuhan, China), phosphorylated Perilipin5 (P-Perilipin5, #9624, Cell Signaling Technology, MA, USA), ATGL (A5126, ABclonal, Wuhan, China), CGI-58 (sc-376931, Santa

Be the First to Write a Review!