Rabbit anti mTOR Polyclonal antibody MBS462086 from MyBioSource.com

Description

The mammalian target of rapamycin (mTOR), also named FKBP12 rapamycin-associated protein (FRAP/RAFT/RAPT/SEP), is a Ser/Thr protein kinase that plays a crucial role in a nutrient-sensitive signalling pathway that regulates cell growth. TOR signalling is potently inhibited by rapamycin, through the direct binding of a FK506-binding protein 12 (FKBP12)/rapamycin complex to the TOR FRB domain, a segment amino terminal to the kinase catalytic domain. mTOR is involved in the regulation of cell growth through initiation of gene translation in response to nutrients such as amino acids (mainly leucine), growth factors, insulin and mitogens. mTOR initiates translation by activating the ribosomal p70S6k protein kinase (S6K1) and by inhibiting the eIF4E inhibitor 4E-BP1. mTOR is phosphorylated at serine 2448 via the phosphatidylinositol 3-kinase (PI3K)/Akt pathway and is autophosphorylated at serine 2481

Product Specs
ItemRabbit anti mTOR Polyclonal antibody
CompanyMyBioSource.com
Price $250.00Supplier Page View Company Product Page
Catalog NumberMBS462086
ConcentrationSize: 100 ug/200 ul
Molecular Weight290 kDa
PurityThe Rabbit IgG is purified by Epitope Affinity Purification
Quantity0.1 mg
HostRabbit
ApplicationsELISA, Western Blot, Immunohistochemistry
ClonalityPolyclonal
FormatThis affinity purified antibody is supplied in sterile phosphatebuffered saline (pH7.2) containing antibody stabilizer
ReactivityHuman, Rat, Mouse, Bovine, Canine, Chicken
Conjugate/TagUnconjugated
ImmunogenThis antibody only recognizes ~290 kDa of human mTOR. It also reacts with mouse and rat mTOR protein. The other species are not tested
TargetmTOR

Add to Compare List

Questions and Answers

What species does this antibody work with, and can it be used for studies involving human mTOR?

The Rabbit anti mTOR Polyclonal antibody is reactive with several species, including human, mouse, rat, bovine, chicken/bird, and canine. It specifically recognizes the ~290 kDa human mTOR protein, making it suitable for studies involving human mTOR.

Can this antibody be used for immunohistochemistry, and what should I consider when using it in this application?

Yes, this antibody is suitable for immunohistochemistry (IHC). When using it in IHC, it is important to optimize the dilution and incubation conditions, as well as to ensure proper fixation and permeabilization of the tissue samples to achieve optimal staining results.

How does the antibody perform in Western blotting, particularly regarding the detection of mTOR?

The Rabbit anti mTOR Polyclonal antibody has been shown to be effective in Western blotting (WB) for detecting mTOR. It recognizes the ~290 kDa protein, which is crucial for studies involving the mTOR signaling pathway, particularly in response to nutrients and growth factors.

Is there any specific information about the immunogen used for this antibody that might affect its specificity?

The antibody is designed to recognize the mTOR protein, which is a Ser/Thr protein kinase involved in cell growth regulation. The immunogen used for this antibody is specific to the mTOR protein, ensuring that it selectively binds to mTOR and not to other proteins, which is critical for accurate experimental results.