Western blot was performed using anti-RORA Polyclonal Antibody (Product # PA1-812) a 58 kDa and was observed corresponding to RORA across the cell lines and tissues tested. Whole cell Extracts of (30 µg lysate) of Hep G2 (Lane 1), OVCAR-3 (Lane 2), HeLa ( Lane 3), Jurkat (Lane 4), IMR-32 (Lane 5), LNCaP (Lane 6), tissue extracts of Mouse Cerebellum (Lane 7) and Rat Brain (Lane 8) were electrophoresed using Novex®), NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1,000 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (lane 1), Jurkat (Lane 2), HEK-293 (lane 3), NTERA-2 (Lane 4), and U-87 MG (Lane 5).The blots were probed with ROR alpha Rabbit Polyclonal Antibody (Product # PA1-812, 1:1000) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 56 kDa band corresponding to ROR alpha was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supplier Page from Thermo Fisher Scientific for RORA Antibody