Cyclophilin 40 Antibody PA3-023 from Thermo Fisher Scientific

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WB

Western blot was performed using Anti-Cyclophilin 40 Polyclonal Antibody (Product # PA3-023) and a ~40 kDa band corresponding to Cyclophilin 40 was observed across cell lines and tissues tested. Whole cell lysate (40 µg lysate) of LNCaP (Lane 1), KARPAS 299 (Lane 2), NIH:OVCAR-3 (Lane 3), K-562 (Lane 4), U-2 OS (Lane 5), A-431 (Lane 6), MCF7 (Lane 7), Mouse Brain (Lane 8) and Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
ICC/IF

Immunofluorescence analysis of Cyclophilin D was done on 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclophilin D Rabbit Polyclonal Antibody (Product # PA3-023) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Cyclophilin 40 Antibody

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Cyclophilin 40 Antibody

The Cyclophilin 40 Antibody from Thermo Fisher Scientific is a Rabbit Polyclonal antibody to PPID. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Cyclophilin 40 Antibody has been shown to work in the following applications: Immunocytochemistry, Super Shift Assay, and Western Blot.

WB

ICC/IF

Cyclophilin 40 Antibody

Cyclophilin 40 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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Cyclophilin 40 Antibody from Thermo Fisher Scientific

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