Western blot analysis was performed on whole cell extracts (30 µg lysate) of A431 (Lane 1), HeLa (Lane 2), A549 (Lane 3), Jurkat (Lane 4), THP-1 (Lane 5) and K562 (Lane 6). The blots were probed with Anti-FOXL2 Rabbit Polyclonal Antibody (Product # PA1-802, 0.1-1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 38 kDa band corresponding to FOXL2 was observed across cell lines tested except A431. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).