Ataxin 7 Antibody PA1-749 from Thermo Fisher Scientific

Product Specs
ItemAtaxin 7 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-749
Quantity100 µg
ApplicationsImmunocytochemistry, Immunohistochemistry (Frozen), Western Blot
Conjugate/TagUnconjugated
FormatPBS with 1mg/mL BSA and 0.05% sodium azide
Concentration1 mg/mL
PurityAntigen affinity chromatography
TargetAtaxin 7
ReactivityDrosophila, Human
HostRabbit
IsotypeIgG
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
Antibody TypePrimary
NCBI Gene AliasesOPCA3, Ataxin7, Dmel\CG9866, ataxin-7, ADCAII, dATXN7, CG9866, SCA7, SGF73
ClonalityPolyclonal
NCBI Full Gene Nameataxin 7, Ataxin 7
ImmunogenSynthetic Peptide: M(1) S E R A A D D V R G E P R R A A(17) C
Storage-20°C, Avoid Freeze/Thaw Cycles

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(1) Increased nuclear import characterizes aberrant nucleocytoplasmic transport in neurons from patients with spinocerebellar ataxia type 7Frontiers in Molecular NeuroscienceNovember 22, 2024Joshua G. Macopson-Jones, Maile Adams, Julien Philippe, Albert R. La SpadaMouse anti-RanGap1 (1:250, Cat. No. sc-28322, Santa Cruz) and Rabbit anti-Ataxin-7 (1:500, Cat. No. PA1-749, Thermo). To control for antibody variability, a follow up stain for nuclear membrane abnormalities

(2) Respiratory neuropathology in spinocerebellar ataxia type 7JCI insightJuly 18, 2024Biswas DD, Shi Y, El Haddad L, Sethi R, Huston M, Kehoe S, Scarrow ER, Strickland LM, Pucci LA, Dhindsa JS, et al.anti-GFAP (1:500; nCor Biotechnology, CPCA-GFAP, AB_2109953), and anti–ataxin-7 (1:500, Invitrogen, PA1-749). Subsequently, the tissues were incubated with anti–goat IgG Alexa Fluor 488 (1:500; Invitrogen, A32814)

(3) AAV-Mediated CAG-Targeting Selectively Reduces Polyglutamine-Expanded Protein and Attenuates Disease Phenotypes in a Spinocerebellar Ataxia Mouse ModelInternational journal of molecular sciencesApril 15, 2024Niewiadomska-Cimicka A, Fievet L, Surdyka M, Jesion E, Keime C, Singer E, Eisenmann A, Kalinowska-Poska Z, Nguyen HHP, Fiszer A, et al.

(4) The 5HT4R agonist velusetrag efficacy on neuropathic chronic intestinal pseudo-obstruction in PrP-SCA7-92Q transgenic miceFrontiers in pharmacologyJanuary 1, 2024Liu Y, Wu Y, Ren D, Tao Y, Mai F, Zhu J, Li X, Colla E, Grimaldi M, Giovannini R, et al.following primary antibodies were used: Hu C/D 1:500 (Abcam, Ab184267, US), Ataxin-7 1:2000 (Thermo Fisher, PA1-749, US), MAP2 1:1,000 (Abcam, ab183830, US), nNOS 1:500 (Abcam, ab76067, US), ChAT 1:1,000 (Abcam, Ab181023,

(5) A novel ubiquitin-proteasome system regulation of Sgf73/ataxin-7 that maintains the integrity of the coactivator SAGA in orchestrating transcriptionGeneticsJuly 6, 2023Barman P, Kaja A, Chakraborty P, Guha S, Roy A, Ferdoush J, Bhaumik SR.

(6) Respiratory dysfunction in a mouse model of spinocerebellar ataxia type 7Disease models & mechanismsJuly 1, 2021Fusco AF, Pucci LA, Switonski PM, Biswas DD, McCall AL, Kahn AF, Dhindsa JS, Strickland LM, La Spada AR, ElMallah MK.dual-stained with anti-ChAT and antiataxin-7 polyclonal antibody (1:500, Invitrogen, Carlsbad, CA, PA1-749) using the same protocol. For negative control staining, tissues were stained with secondary antibodies

(7) DNAzyme Cleavage of CAG Repeat RNA in Polyglutamine DiseasesNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeuticsJuly 1, 2021Zhang N, Bewick B, Schultz J, Tiwari A, Krencik R, Zhang A, Adachi K, Xia G, Yun K, Sarkar P, et al.Cell Signal Technology), mouse anti-8-OHdG (E-8, 1:200, Santa Cruz Biotechnology), rabbit anti-ATXN7 (PA1-749, 1:1,000, Thermo Fisher Scientific), mouse anti-TBP (1TBP18, 1:1,000, Thermo Fisher Scientific), and

(8) SCA7 Mouse Cerebellar Pathology Reveals Preferential Downregulation of Key Purkinje Cell-Identity Genes and Shared Disease Signature with SCA1 and SCA2The Journal of neuroscience : the official journal of the Society for NeuroscienceJune 2, 2021Niewiadomska-Cimicka A, Doussau F, Perot JB, Roux MJ, Keime C, Hache A, Piguet F, Novati A, Weber C, Yalcin B, et al.The following primary antibodies were applied overnight at 4°C: anti-ATXN7, 1:1 000 (ThermoFisher, PA1-749); anti-H2B, 1:10 000 (IGBMC clone H2-2A8); anti-H2Bub 1:2 000 (MediMabs, MM-0029); anti-H3K9ac 1:2 000

(9) Metabolic and Organelle Morphology Defects in Mice and Human Patients Define Spinocerebellar Ataxia Type 7 as a Mitochondrial DiseaseCell reportsJanuary 29, 2019Ward JM, Stoyas CA, Switonski PM, Ichou F, Fan W, Collins B, Wall CE, Adanyeguh I, Niu C, Sopher BL, et al.(Sigma-Aldrich) for at least 1 hr at RT. Membranes were then probed with rabbit anti-ATXN7 (Thermo, PA1-749) 1:1000, mouse anti-polyQ 1C2 (Millipore MAB1574), rabbit anti-TOMM20 (Novus NBP1-81556) 1:1000, mouse

(10) Mutant CAG Repeats Effectively Targeted by RNA Interference in SCA7 CellsGenesDecember 17, 2016Fiszer A, Wroblewska JP, Nowak BM, Krzyzosiak WJ.USA). 2.5. Immunofluorescence Immunofluorescence labeling was performed with an anti-ataxin-7 antibody (PA1-749, ThermoFisher Scientific) at a 1:100 dilution and secondary anti-rabbit antibody conjugated with Alexa

(11) Lentiviral vector-mediated overexpression of mutant ataxin-7 recapitulates SCA7 pathology and promotes accumulation of the FUS/TLS and MBNL1 RNA-binding proteinsMolecular neurodegenerationJuly 28, 2016Alves S, Marais T, Biferi MG, Furling D, Marinello M, El Hachimi K, Cartier N, Ruberg M, Stevanin G, Brice A, et al.Primary antibodies,Source/Reference catalog,WB,IHC rabbit anti-ataxin-7,Thermo Scientific/(PA1-749),1:2000,1:5000 rabbit-anti-ubiquitin,Dako/(Z0458),–,1:2000 mouse anti-ataxin-7 (1C1),Provided by Dr

(12) Proteolytic cleavage of ataxin-7 promotes SCA7 retinal degeneration and neurological dysfunctionHuman molecular geneticsJuly 15, 2015Guyenet SJ, Mookerjee SS, Lin A, Custer SK, Chen SF, Sopher BL, La Spada AR, Ellerby LM.

(13) Nonallele specific silencing of ataxin-7 improves disease phenotypes in a mouse model of SCA7Molecular therapy : the journal of the American Society of Gene TherapySeptember 1, 2014Ramachandran PS, Boudreau RL, Schaefer KA, La Spada AR, Davidson BL.

(14) RNA interference-based therapy for spinocerebellar ataxia type 7 retinal degenerationPloS oneJanuary 1, 2014Ramachandran PS, Bhattarai S, Singh P, Boudreau RL, Thompson S, Laspada AR, Drack AV, Davidson BL.Western blots were performed using the polyclonal rabbit anti-ATXN7 (1:1000; Thermo Fisher Scientific #PA1-749) and b actin (1:10000, Sigma, A5441) and quantification was performed using Image J software. AAV injections SCA7

(15) Histone deacetylase-3 interacts with ataxin-7 and is altered in a spinocerebellar ataxia type 7 mouse modelMolecular neurodegenerationOctober 27, 2013Duncan CE, An MC, Papanikolaou T, Rugani C, Vitelli C, Ellerby LM.containing 5% milk for 1 h. Primary antibodies were diluted in blocking solution at 1:1000 for ataxin-7 (PA1-749, Thermo Scientific, Rockford, IL), 1:100 for HDAC3 (sc-17795, Santa Cruz Biotechnology), 1:5000 for

(16) Interferon β induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in miceBrain : a journal of neurologyJune 1, 2013Chort A, Alves S, Marinello M, Dufresnois B, Dornbierer JG, Tesson C, Latouche M, Baker DP, Barkats M, El Hachimi KH, et al.

(17) Reduction of mutant ataxin-7 expression restores motor function and prevents cerebellar synaptic reorganization in a conditional mouse model of SCA7Human molecular geneticsMarch 1, 2013Furrer SA, Waldherr SM, Mohanachandran MS, Baughn TD, Nguyen KT, Sopher BL, Damian VA, Garden GA, La Spada AR.

(18) Spinocerebellar ataxia type 7 cerebellar disease requires the coordinated action of mutant ataxin-7 in neurons and glia, and displays non-cell-autonomous bergmann glia degenerationThe Journal of neuroscience : the official journal of the Society for NeuroscienceNovember 9, 2011Furrer SA, Mohanachandran MS, Waldherr SM, Chang C, Damian VA, Sopher BL, Garden GA, La Spada AR.1:1000 dilution; anti-S100␤ antibody (ab11178, Abcam) at 1:1000 dilution; or anti-ataxin-7 antibody (PA1-749, Affinity Bioreagents) at 1:500 dilution in PBS with 5% NGS and 0.1% Triton X-100. After three washes

(19) Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragmentThe Journal of neuroscience : the official journal of the Society for NeuroscienceDecember 2, 2009Mookerjee S, Papanikolaou T, Guyenet SJ, Sampath V, Lin A, Vitelli C, DeGiacomo F, Sopher BL, Chen SF, La Spada AR, et al.for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (RDI-TRK5G46C5, Research Diagnostics); 1:1000 for ataxin-7 (PA1-749, Affinity Bioreagents, directed against N-terminal 17 aa of mouse and human ataxin-7), 1:200 for HDAC7

(20) Proteolytic cleavage of ataxin-7 by caspase-7 modulates cellular toxicity and transcriptional dysregulationThe Journal of biological chemistryOctober 12, 2007Young JE, Gouw L, Propp S, Sopher BL, Taylor J, Lin A, Hermel E, Logvinova A, Chen SF, Chen S, et al.

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Ataxin 7 Antibody from Thermo Fisher Scientific

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Ataxin 7 Antibody from Thermo Fisher Scientific

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