Glucocorticoid Receptor alpha Antibody PA1-516 from Thermo Fisher Scientific

Product Specs
ItemGlucocorticoid Receptor alpha Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-516
NCBI Gene AliasesGR, Grl-1, Gcr, GCCR, GCR, GCRST, Grl, GRL, Grl1
Quantity50 µg
ClonalityPolyclonal
HostRabbit
Conjugate/TagUnconjugated
ApplicationsChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetGlucocorticoid Receptor alpha
FormatPBS with 0.05% sodium azide
NCBI Full Gene Namenuclear receptor subfamily 3 group C member 1, nuclear receptor subfamily 3, group C, member 1
Concentration1 mg/mL
ImmunogenSynthetic Peptide: C(755) E I I T N Q I P K Y S N G N I K K(771)
IsotypeIgG
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage-20°C, Avoid Freeze/Thaw Cycles

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) STING pathway contributes to Steroid-Hyporesponsive Lung Inflammation in DSS-induced colitis mice modelPLoS OneMarch 27, 2026Mariam Wed Eladham, Narjes Saheb Sharif-Askari, Bushra Mdkhana, Shirin Ali, Baraa Khalid Salah Al-Sheakly, Nival Ali, Balachandar Selvakumar, Fatemeh Saheb Sharif-Askari, Ibrahim Hachim, Rabih Halwani(cat. #4970, 1:1000) (Cell Signaling Technologies, Danvers, MA), and anti-glucocorticoid receptor α (PA1–516), anti-glucocorticoid receptor β (PA3–514) (Thermo Fisher Scientific, Waltham, MA) at 4◦C overnight.

(2) Altered levels of angiogenin and tRNA-derived fragments associate with severe asthmaSci RepMay 29, 2025Clara Claus, Julieta B. Grosso, María Belen Maraval, Matias Ardusso, Oscar A. Bottasso, Tania Maes, Ken Bracke, Guy Brusselle, Mauro Ibañez, Ledit R. F. Ardusso, et al.incubated overnight (4 °C) with primary polyclonal antibody against GRalpha (Thermo Scientific cat #PA1-516) or Isotype negative control (rabbit Ig (Novus #NB810-56910). Anti rabbit-HRP secondary antibody (Klinipath

(3) Regulation of meristem and hormone function revealed through analysis of directly-regulated SHOOT MERISTEMLESS target genesSci RepJanuary 2, 2025Tamara Lechon, Nicholas A. Kent, James A. H. Murray, Simon Scofieldused to pre-clear the digested chromatin followed by the anti-glucocorticoid receptor alpha antibody PA1-516 (Thermo Fisher) for immunoprecipitation (IP) or BSA for no-antibody mock-immunoprecipitation (NoAB)

(4) Genetic variants and haplotypes in fibulin-5 (FBLN5) are associated with pseudoexfoliation glaucoma but not with pseudoexfoliation syndromeBioscience reportsMarch 31, 2023Kapuganti RS, Bharati B, Mohanty PP, Alone DP.For supershift assays, EMSA-specific antibodies for TFII I (sc-46670X, Santa Cruz, U.S.A.) and GR-α (PA1516, Invitrogen) were pre-incubated with the nuclear extract for 1 h before adding the final reaction mixture.

(5) TNF-α Regulates the Glucocorticoid Receptor Alpha Expression in Human Nasal Epithelial Cells Via p65-NF-κb and p38-MAPK Signaling PathwaysIranian journal of biotechnologyJanuary 1, 2023Jiang Y, Liu B, Bao X, Zhou P, Li J.membrane was incubated with blocking buffer and incubated with GRα antibody ((4 μg.mL-1; Invitrogen, PA1516, USA), GRβ antibody (1:500; Invitrogen, PA3-514), p-P65 NF-κB (1:1000; Cell Signaling Technology, 3033T),

(6) Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitisJournal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-facialeJune 11, 2022Shao S, Wang Y, Zhao Y, Xu Y, Wang T, Du K, Bao S, Wang X, Zhang L.ChemiDoc™ Imaging System (Bio-Rad, USA). The following antibodies were used for WB analysis: anti-GRα (1:1000, Invitrogen, PA1-516), anti-GR (1:1000, Invitrogen, PA1-511A), anti-β-actin (1:10,000, Sigma, A5441), goat anti-mouse (1:10,000,

(7) Salt-Sensitive Hypertension in GR+/− Rats Is Accompanied with Dysregulation in Adrenal Soluble Epoxide Hydrolase and Polyunsaturated Fatty Acid PathwaysInternational Journal of Molecular SciencesDecember 8, 2021Paul-Emmanuel Vanderriele et al.((rabbit anti-GR (N-terminal, M-20; Santa Cruz, CA, USA), rabbit anti-GR (C-terminal amino-acids 755–771, PA1-516, Thermo Fischer Scientific)). sEH expression was analysed in 30 µg of protein lysate (rabbit anti-sEH,

(8) Twist1 contributes to developing and sustaining corticosteroid resistance in ulcerative colitisTheranosticsJanuary 1, 2021Liu C, Mo LH, Feng BS, Jin QR, Li Y, Lin J, Shu Q, Liu ZG, Liu Z, Sun X, et al.CA). Reagents and materials for immunoprecipitation, RT-qPCR and Western blotting, GRβ (PA3-514), GRα (PA1-516) Abs were purchased from Invitrogen (Carlsbad, CA). Proteins of IL-6 and TGF-β, ELISA kits for determining

(9) Spatiotemporal control of axillary meristem formation by interacting transcriptional regulatorsDevelopment (Cambridge, England)December 10, 2018Zhang C, Wang J, Wenkel S, Chandler JW, Werr W, Jiao Y.control. Immunoprecipitations were performed using an antibody against the glucocorticoid receptor (GR) (PA1 516; Affinity Bioreagents) or against GFP (11814460001, Roche). The precipitated DNA was isolated, purified

(10) Corticosteroid receptors adopt distinct cyclical transcriptional signaturesFASEB journal : official publication of the Federation of American Societies for Experimental BiologyOctober 1, 2018Le Billan F, Amazit L, Bleakley K, Xue QY, Pussard E, Lhadj C, Kolkhof P, Viengchareun S, Fagart J, Lombès M.

(11) Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory networkDevelopment (Cambridge, England)April 30, 2018Scofield S, Murison A, Jones A, Fozard J, Aida M, Band LR, Bennett M, Murray JAH.performed according to Morohashi et al. (2009), using the anti-glucocorticoid receptor alpha antibody PA1-516 (Thermo Fisher). p35S::STM-GR plants were grown on 60 µM DEX for 9 days and aerial tissue harvested.

(12) The bHLH transcription factor SPATULA enables cytokinin signaling, and both activate auxin biosynthesis and transport genes at the medial domain of the gynoeciumPLoS geneticsApril 1, 2017Reyes-Olalde JI, Zúñiga-Mayo VM, Serwatowska J, Chavez Montes RA, Lozano-Sotomayor P, Herrera-Ubaldo H, Gonzalez-Aguilera KL, Ballester P, Ripoll JJ, Ezquer I, et al.enough material was collected. Glucocorticoid Receptor alpha polyclonal antibody (Thermo Scientific; PA1-516) (2 μg per sample) was used to immunoprecipitate ARR1ΔDDK-GR complexes. Results from qPCR experiments

(13) Annexin A1 in blood mononuclear cells from patients with coronary artery disease: Its association with inflammatory status and glucocorticoid sensitivityPloS oneJanuary 1, 2017Bergström I, Lundberg AK, Jönsson S, Särndahl E, Ernerudh J, Jonasson L.were stained with rabbit anti-human GR-alpha or GR-beta antibodies (ThermoFischer, Waltham, MA, USA; PA1-516 and PA3-514 respectively) for 30 min in 4°C in the dark. After washing in Permeabilization buffer, cells

(14) The Role of S-Palmitoylation of the Human Glucocorticoid Receptor (hGR) in Mediating the Nongenomic Glucocorticoid ActionsJournal of molecular biochemistryJanuary 1, 2017Nicolaides NC, Kino T, Roberts ML, Katsantoni E, Sertedaki A, Moutsatsou P, Psarra AG, Chrousos GP, Charmandari E.anti-hGRα antibody (dilution 1:100 in 0.5% BSA-PBS) (polyclonal anti-rabbit immunoglobulin, Cat No. PA1-516, Thermo Fisher Scientific, Boston, MA, USA) for 2 hours and subsequently with secondary antibody (dilution

(15) Three Novel Heterozygous Point Mutations of NR3C1 Causing Glucocorticoid ResistanceHuman mutationAugust 1, 2016Vitellius G, Fagart J, Delemer B, Amazit L, Ramos N, Bouligand J, Le Billan F, Castinetti F, Guiochon-Mantel A, Trabado S, et al.

(16) Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in ArabidopsisPLoS geneticsJuly 1, 2016Shi B, Zhang C, Tian C, Wang J, Wang Q, Xu T, Xu Y, Ohno C, Sablowski R, Heisler MG, et al.control. Immunoprecipitations were performed with a polyclonal antibody against GR (Affinity Bioreagents, PA1-516), a monoclonal antibody against HA (Beyotime, AH158), a polyclonal antibody against H3K27me3 (Millipore,

(17) Additive anti-inflammatory effects of corticosteroids and phosphodiesterase-4 inhibitors in COPD CD8 cellsRespiratory researchJanuary 25, 2016Grundy S, Plumb J, Kaur M, Ray D, Singh D.

(18) A kinase-independent activity of Cdk9 modulates glucocorticoid receptor-mediated gene inductionBiochemistryMarch 25, 2014Zhu R, Lu X, Pradhan M, Armstrong SP, Storchan GB, Chow CC, Simons SS Jr.

(19) Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower developmentGenome biologyMarch 3, 2014Pajoro A, Madrigal P, Muiño JM, Matus JT, Jin J, Mecchia MA, Debernardi JM, Palatnik JF, Balazadeh S, Arif M, et al.previously published protocol [46] using an anti-GR antibody (Glucocorticoid Receptor alpha Polyclonal antibody (PA1-516, Thermo Scientific), to precipitate AP1-GR), or a peptide SEP3 antibody [36]. 0.75 g of plant material

(20) Glucocorticoid signaling in myeloid cells worsens acute CNS injury and inflammationThe Journal of neuroscience : the official journal of the Society for NeuroscienceMay 1, 2013Sorrells SF, Caso JR, Munhoz CD, Hu CK, Tran KV, Miguel ZD, Chien BY, Sapolsky RM.

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Glucocorticoid Receptor alpha Antibody from Thermo Fisher Scientific

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Glucocorticoid Receptor alpha Antibody from Thermo Fisher Scientific

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