GnRH1 Antibody PA1-121 from Thermo Fisher Scientific

Product Specs
ItemGnRH1 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-121
TargetGnRH1
ApplicationsImmunohistochemistry (Paraffin)
Quantity100 µg
ReactivityAmphibian, Mouse
ClonalityPolyclonal
HostRabbit
FormatPBS with 1mg/mL BSA and 0.05% sodium azide
Conjugate/TagUnconjugated
Concentration1 mg/mL
ImmunogenSynthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
Storage-20°C, Avoid Freeze/Thaw Cycles
IsotypeIgG
NCBI Full Gene Namegonadotropin releasing hormone 1
NCBI Gene AliasesGnrh, Gnrh2, LHRH, Lhrh1, Lnrh, hpg
Antibody TypePrimary
PurityAntigen affinity chromatography

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Thermo Fisher Scientific

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Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Host-specificity assessment of feline alphaherpesvirus-1 derived immunocontraceptive candidates in non-feline modelsVirol JMarch 4, 2026Ellen Cottingham, Natali Krekeler, Thurid Johnstone, Carol Hartley, Joanne Devlinand 50 µL added to wells as the primary antibody. Polyclonal antibodies to GnRH (ThermoFisher Cat # PA1-121) at 1/500 dilution in PBS-T were used as a positive control primary antibody for the GnRH ELISA. The

(2) Selective effects of protein 4.1N deficiency on neuroendocrine and reproductive systemsScientific reportsOctober 12, 2020Wang H, Parra M, Conboy JG, Hillyer CD, Mohandas N, An X.dilution of 1:100 for anti-4.1NHP, 1:50 for anti-GH(R&D systems, BAF1067), 1:50 for anti-GnRH (Thermo, PA1-121)39, 1:200 for anti-GHRH(Abcam, Ab48617), overnight at 4 °C. After several washes in PBS, the sections

(3) The Homeodomain Transcription Factors Vax1 and Six6 Are Required for SCN Development and FunctionMolecular neurobiologyFebruary 1, 2020Pandolfi EC, Breuer JA, Nguyen Huu VA, Talluri T, Nguyen D, Lee JS, Hu R, Bharti K, Skowronska-Krawczyk D, Gorman MR, et al.previously described [55]. Briefly, the primary antibody used was rabbit anti-GnRH (Thermo Scientific #PA1–121, dilution 1/1000, RRID:AB_325077), rabbit anti-GnRH (Novus #NBP2–22444, 1:1000, or Immunostar #20075,

(4) Differential CRE Expression in Lhrh-cre and GnRH-cre Alleles and the Impact on Fertility in Otx2-Flox MiceNeuroendocrinologyJanuary 1, 2019Hoffmann HM, Larder R, Lee JS, Hu RJ, Trang C, Devries BM, Clark DD, Mellon PL.The primary antibodies used for single immunohistochemistry are rabbit anti-GnRH (Thermo Scientific #PA1–121, dilution 1/1,000, RRID:AB_325077), chicken anti-beta galactosidase (Abcam #AB9361, dilution 1/1,000,

(5) Diet-Induced Obesity Elicits Macrophage Infiltration and Reduction in Spine Density in the Hypothalami of Male but Not Female MiceFrontiers in immunologyJanuary 1, 2018Lainez NM, Jonak CR, Nair MG, Ethell IM, Wilson EH, Carson MJ, Coss D.AB_1904154 MAP2 chicken 1:5,000 Abcam, ab5392; AB_2138153 GnRH rabbit 1:1,000 ThermoFisher, PA1-121; AB_325077 β-tubulin rabbit 1:1,000 Santa Cruz Biotechnology, sc-9104; AB_2241191 CD45 APC-eFluor 780 rat 1:300 eBioscience,

(6) Sox21 deletion in mice causes postnatal growth deficiency without physiological disruption of hypothalamic-pituitary endocrine axesMolecular and cellular endocrinologyJanuary 5, 2017Cheung LYM, Okano H, Camper SA.anti-mouse GHRH (gift from Frank Talamantes and Malcolm Low) at 1:1000; rabbit anti-mouse GNRH (ThermoFisher PA1-121) at 1:200. 2.4 In situ hybridization In situ hybridization for Trh was performed on frozen brain sections

(7) Deletion of Vax1 from Gonadotropin-Releasing Hormone (GnRH) Neurons Abolishes GnRH Expression and Leads to Hypogonadism and InfertilityThe Journal of neuroscience : the official journal of the Society for NeuroscienceMarch 23, 2016Hoffmann HM, Trang C, Gong P, Kimura I, Pandolfi EC, Mellon PL.citrate. Briefly, the primary antibody used was rabbit anti-GnRH (1:1000; Thermo Fisher Scientific, PA1-121) or rabbit anti-GnRH (1:1000; Novus, NBP222444). GnRH-positive neurons were counted throughout the brain. The

(8) Distribution and regulation of gonadotropin-releasing hormone, kisspeptin, RF-amide related peptide-3, and dynorphin in the bovine hypothalamusPeerJJanuary 1, 2016Tanco VM, Whitlock BK, Jones MA, Wilborn RR, Brandebourg TD, Foradori CD.Alternate sections were then incubated with polyclonal antibodies against GnRH (rabbit host; 1:10,000; PA1-121; ThermoFisher Scientific), kisspeptin (rabbit host; 1:30,000; generously provided by Alain Caraty, Institut

(9) Olfactomedin 1 Deficiency Leads to Defective Olfaction and Impaired Female FertilityEndocrinologySeptember 1, 2015Li R, Diao H, Zhao F, Xiao S, El Zowalaty AE, Dudley EA, Mattson MP, Ye X.

(10) In Utero Development of Kisspeptin/GnRH Neural Circuitry in Male MiceEndocrinologySeptember 1, 2015Kumar D, Periasamy V, Freese M, Voigt A, Boehm U.

(11) Gonadotropin and kisspeptin gene expression, but not GnRH, are impaired in cFOS deficient miceMolecular and cellular endocrinologyAugust 15, 2015Xie C, Jonak CR, Kauffman AS, Coss D.serum, 0.3% Triton X-100) for 45 minutes and incubated with primary antibodies against GnRH (1:1000, PA1-121, Pierce, Thermo Rockford, IL) overnight at 4 °C. After washing, slides were incubated with biotinylated

(12) Chronic oestradiol reduces the dendritic spine density of KNDy (kisspeptin/neurokinin B/dynorphin) neurones in the arcuate nucleus of ovariectomised Tac2-enhanced green fluorescent protein transgenic miceJournal of neuroendocrinologyApril 1, 2015Cholanian M, Krajewski-Hall SJ, McMullen NT, Rance NE.homogenates (Aves Labs). This GFP antibody did not label neurones in wildtype mouse tissue (30). The GnRH antibody (PA1–121, Lot no. MD150614, Thermo Scientific, Rockford, IL) was raised in rabbit against a synthetic peptide

(13) Conditional genetic transsynaptic tracing in the embryonic mouse brainJournal of visualized experiments : JoVEDecember 22, 2014Kumar D, Boehm U.

(14) Murine arcuate nucleus kisspeptin neurons communicate with GnRH neurons in uteroThe Journal of neuroscience : the official journal of the Society for NeuroscienceMarch 5, 2014Kumar D, Freese M, Drexler D, Hermans-Borgmeyer I, Marquardt A, Boehm U.TNB blocking solution. The primary antisera used were (1) rabbit antibody to GnRH (1:1000; catalog #PA1-121, Affinity BioReagents), (2) rabbit antibody to estrogen receptor ␣ (ER␣; 1:1000; catalog #06-935, Millipore), (3)

(15) Gene dosage of Otx2 is important for fertility in male miceMolecular and cellular endocrinologySeptember 5, 2013Larder R, Kimura I, Meadows J, Clark DD, Mayo S, Mellon PL.as previously described [16]. The primary antibody used was anti-GnRH antibody (Affinity BioReagents PA1-121; 1:1000 dilution). Biotinylated goat-anti-rabbit IgG (Vector Laboratories, 1:300 dilution) was used

(16) Developmental profile and sexually dimorphic expression of kiss1 and kiss1r in the fetal mouse brainFrontiers in endocrinologyJanuary 1, 2013Knoll JG, Clay CM, Bouma GJ, Henion TR, Schwarting GA, Millar RP, Tobet SA.by IHC for GnRH. Primary antibody for these experiments was rabbit-anti-GnRH (Affinity Bioreagents #PA1-121) used at a concentration of 1:200. For visualization, the IHC was developed using Cy3-conjugated streptavidin

(17) Lhx2-dependent specification of olfactory sensory neurons is required for successful integration of olfactory, vomeronasal, and GnRH neuronsFASEB journal : official publication of the Federation of American Societies for Experimental BiologyAugust 1, 2012Berghard A, Hägglund AC, Bohm S, Carlsson L.

(18) Genetic identification of GnRH receptor neurons: a new model for studying neural circuits underlying reproductive physiology in the mouse brainEndocrinologyApril 1, 2011Wen S, Götze IN, Mai O, Schauer C, Leinders-Zufall T, Boehm U.

(19) Hypothalamic dysregulation and infertility in mice lacking the homeodomain protein Six6The Journal of neuroscience : the official journal of the Society for NeuroscienceJanuary 12, 2011Larder R, Clark DD, Miller NL, Mellon PL.described (Miller et al., 2009). The primary antibody used was anti-GnRH antibody (Affinity Bioreagents; PA1-121; 1:1000 dilution in 5% goat serum/0.3% Triton X-100). Biotinylated goat anti-rabbit IgG (Vector Laboratories;

(20) Composition of the migratory mass during development of the olfactory nerveThe Journal of comparative neurologyDecember 15, 2010Miller AM, Treloar HB, Greer CA.NH2 (32) of mouse GnRH,"Affinity BioReagents (now Thermo Scientific Pierce Antibodies, Rockford, IL) (PA1-121)","1:1,000","Western blot from mouse brain has an expected band at 10.4 kDa. Staining with GnRH produced

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GnRH1 Antibody

GnRH1 Antibody from Thermo Fisher Scientific

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GnRH1 Antibody from Thermo Fisher Scientific

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