Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), LNCap (Lane 2), PC-3 (Lane 3), MCF-7(Lane 4), MDA-MB-231 (Lane 5), HeLa (Lane 6), tissue extracts of Mouse testis (Lane 7) and Rat testis (Lane 8). The blot was probed with Anti-ERp57 Rabbit Polyclonal Antibody (Product # PA3-009, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 57 kDa band corresponding to ERp57 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).