Fig 2: Flow cytometric analysis of the fluorescence transferred by PEVs into lymphocytes. The staining of directly labeled cells and the negative control cells (gray line) after 30 min, 7, and 20 h of incubation are shown. (A) The kinetics of PEVs uptaken by B CD21+ lymphocytes and (B) PEVs uptake by T CD3+ Lymphocytes.

Fig 3: Changes in frequencies of canine CD8+ (A) and CD4+ (B) lymphocytes after 5 days culture of PBMCs in a 37°C, 5% CO2 environment with ConA and IL-2 presence and PEV1/ PEV2 or without PEVs (n = 8). The results are presented as the mean ± SEM.

Fig 4: Changes in frequencies of canine CD4+IFN?+ and CD8+IFN?+ and CD4+IL17+ lymphocytes after 5 days of culture of PBMCs in a 37 °C, 5% CO2 environment with ConA and IL-2 presence and PEV1/ PEV2 or without PEVs (n = 8). The results are presented as the mean ± SEM.

Fig 5: The figures show the proliferation intensity of CD3+ (A) and CD3-CD21+ (B) lymphocytes after 5 days of culture of PBMC in a 37 °C, 5% CO2 environment with ConA and IL-2 presence and PEV1/PEV2 or without PEVs (n = 8). The results are presented as the mean ± SEM.