RAT ANTI MOUSE F4/80:Alexa Fluor® 488 MCA497A488 from Bio-Rad (AbD Serotec - FAB)

Product Specs
ItemRAT ANTI MOUSE F4/80:Alexa Fluor® 488
CompanyBio-Rad
Price $428.00Supplier Page View Company Product Page
Catalog NumberMCA497A488
Quantity100 TESTS/1ml
ApplicationsFlow Cytometry
ConcentrationIgG concentration 0.05 mg/ml
Conjugate/TagALEXA FLUOR® 488
ReactivityMouse
TargetF4/80
IsotypeIgG2b
CloneCl:A3-1
HostRat
NCBI Full Gene Nameadhesion G protein-coupled receptor E1
NCBI Gene AliasesEmr1, DD7A5-7, Ly71, Gpf480, F4/80, EGF-TM7, TM7LN3
ClonalityMonoclonal Antibody

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Disentangling neuroimmune landscapes across peripheral activation paradigms resolves divergent glial state programsResearch SquareMarch 11, 2026Mahesh Chandra Kodali, Zhengjun Wang, Geng Lin, Francesca-Fang Liao(Miltenyi Biotec, 130-116-249), 1:25 Rat anti mouse Alexa Fluor® 488 F4/80 antibody Clone A3-1(Biorad, MCA497A488), Rat Anti-Human/Mouse TREM-2 PE-conjugated Monoclonal Antibody Clone 237920 (Novus Bio, FAB17291P).

(2) Boosting hydrogel conductivity via water-dispersible conducting polymers for injectable bioelectronicsNat CommunApril 22, 2025Hossein Montazerian, Elham Davoodi, Canran Wang, Farnaz Lorestani, Jiahong Li, Reihaneh Haghniaz, Rohan R. Sampath, Neda Mohaghegh, Safoora Khosravi, Fatemeh Zehtabi, et al.implantation samples, immunofluorescent staining was performed. Anti-mouse F4/80 (BioRad, catalog number: MCA497A488, lot #18567, clone #Cl:A3-1), CD80 (BioLegend, catalog number: cat 600055, lot #b444189, clone W17200C),

(3) Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitisNeural regeneration researchMarch 1, 2024Liu C, Guo S, Liu R, Guo M, Wang Q, Chai Z, Xiao B, Ma C.antibodies diluted in 1% BSA-PBS buffer: the macrophage marker Alexa Fluor 488-anti-F4/80 (Bio-Rad, Cat# MCA497A488, RRID: AB_321210), the pro-inflammatory M1 macrophage subtype marker PE-CD16/32, and the anti-inflammatory

(4) The Voltage-Gated Hv1 H(+) Channel Is Expressed in Tumor-Infiltrating Myeloid-Derived Suppressor CellsInternational journal of molecular sciencesMarch 25, 2023Cozzolino M, Gyöngyösi A, Korpos E, Gogolak P, Naseem MU, Kállai J, Lanyi A, Panyi G.Ly6G-APC,1A8,BioLegend,,IF 8,Rat anti MHCII-PercP-Cy5,M5/114.15.2,BioLegend,107626,FACS 9,Rat anti F4/80 -AF488,CI:A3-1,BioRad,MCA497A488,FACS 10,Hamster anti mouse CD3e,145-2C11,Kind gift fromProf. Jo A. Van Ginderachter,,T cell proliferation 11,Rat

(5) β-Catenin-NF-κB-CFTR interactions in cholangiocytes regulate inflammation and fibrosis during ductular reactioneLifeOctober 5, 2021Hu S, Russell JO, Liu S, Cao C, McGaughey J, Rai R, Kosar K, Tao J, Hurley E, Poddar M, et al.cleaved caspase 3 (Cell Signaling, 9664, 1:100), p-Erk1/2 (Cell Signaling, 4370, 1:100), F4/80 (Bio-Rad, MCA497A488, 1:100) for 1 hr at room temperature or at 4°C overnight: β-catenin (Abcam, ab32572, 1:50) and p21 (Santa Cruz

(6) Obesity accelerates hair thinning by stem cell-centric converging mechanismsNatureJuly 1, 2021Morinaga H, Mohri Y, Grachtchouk M, Asakawa K, Matsumura H, Oshima M, Takayama N, Kato T, Nishimori Y, Sorimachi Y, et al.(Biolegend, 833904, 1:300), NFκB (Santa Cruz, sc-101749, 1:50), CD11b (BD, 557686, 1:100), F4/80 (Bio-Rad, MCA497A488, 1:100), KI67 (Invitrogen, 14-5698-82, 1:300), CD3 (Biolegend, 100235, 1:100), MHC2 (Biolegend, 107605,

(7) Counter Regulation of Spic by NF-κB and STAT Signaling Controls Inflammation and Iron Metabolism in MacrophagesCell reportsJune 30, 2020Alam Z, Devalaraja S, Li M, To TKJ, Folkert IW, Mitchell-Velasquez E, Dang MT, Young P, Wilbur CJ, Silverman MA, et al.107635 Anti-mouse-APC-CD11b antibody,Biolegend,Cat# 101212 Anti-mouse-AF488-F4/80 antibody,Bio Rad,Cat# MCA497A488 Anti-mouse-Percpcy5.5-Ly6G antibody,Biolegend,Cat# 127615 Anti-mouse-APC-F4/80 antibody,Invitrogen,Cat#

(8) TGFβ blocks IFNα/β release and tumor rejection in spontaneous mammary tumorsNature communicationsSeptember 11, 2019Guerin MV, Regnier F, Feuillet V, Vimeux L, Weiss JM, Bismuth G, Altan-Bonnet G, Guilbert T, Thoreau M, Finisguerra V, et al.was performed at 37 °C for 15 min with antibodies specific for F4/80-AF488 (#MCA497A647) or AF647 (#MCA497A488) both purchased from Bio Rad, EpCAM-BV421 (#563214), CD31-Biot (#553371) both from BD Pharmingen or

(9) Aged Mouse Cortical Microglia Display an Activation Profile Suggesting Immunotolerogenic FunctionsInternational journal of molecular sciencesMarch 1, 2018Zöller T, Attaai A, Potru PS, Ruß T, Spittau B.Germany), Cd45 (1:20, 17-0451-82, eBiosciene), Cd86 (5 µL, MCA2463PE, AbD Serotech) and F4/80 (5 µL, MCA497A488, AbD Serotech) at 4 ◦ C for 15 min. Fc (fragment crystallizable region) receptor blocking (TrueStain

(10) Green Tea Polyphenol EGCG Upregulates Tollip Expression by Suppressing Elf-1 ExpressionJournal of immunology (Baltimore, Md. : 1950)November 1, 2017Kumazoe M, Yamashita M, Nakamura Y, Takamatsu K, Bae J, Yamashita S, Yamada S, Onda H, Nojiri T, Kangawa K, et al.A21428) was purchased from Thermo Fisher Scientific. An Alexa Fluor 488–labeled F4/80 Ab (catalog number MCA497A488) was obtained from Bio-Rad (Hercules, CA). Tollip expression vector (catalog number MC209983) was purchased

(11) A chloroquine-induced macrophage-preconditioning strategy for improved nanodeliveryScientific reportsOctober 23, 2017Wolfram J, Nizzero S, Liu H, Li F, Zhang G, Li Z, Shen H, Blanco E, Ferrari M.normal horse serum (Vector Laboratories), and incubated with a F4/80 antibody (1:100 dilution; Bio-Rad, #MCA497A488) at 4 °C overnight. The slides were then incubated with an anti-rat IgG-Alexa Fluor-488 (1:200 dilution;

(12) Contribution of Kupffer cells to liposome accumulation in the liverColloids and Surfaces B: BiointerfacesOctober 1, 2017Emma Samuelsson, Haifa Shen, Elvin Blanco, Mauro Ferrari, Joy Wolframsaline (PBS; HyClone) from Thermo Fisher Scientific; F4/80: Alexa Fluor 647 antibody from Bio-Rad (MCA497A488); CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay from Promega; Microtainer Tubes with

(13) VEGF blockade enhances the antitumor effect of BRAFV600E inhibitionEMBO molecular medicineFebruary 1, 2017Comunanza V, Corà D, Orso F, Consonni FM, Middonti E, Di Nicolantonio F, Buzdin A, Sica A, Medico E, Sangiolo D, et al.anti‐CD45 Alexa Fluor 647‐conjugated (#103124; Biolegend); anti‐CD68 (#ab125212; Abcam); anti‐F4/80 (#MCA497A488; Bio‐Rad); anti‐collagen I (#ab21286; Abcam); anti‐LOX (#ab31238; Abcam); anti‐α‐SMA Cy3‐conjugated

(14) Minimal asbestos exposure in germline BAP1 heterozygous mice is associated with deregulated inflammatory response and increased risk of mesotheliomaOncogeneApril 14, 2016Napolitano A, Pellegrini L, Dey A, Larson D, Tanji M, Flores EG, Kendrick B, Lapid D, Powers A, Kanodia S, et al.(leukocytes; anti-CD45-BV711, 563709, BD Biosciences, San Jose, CA, USA), F4/80 (MΦ; anti-F4/80-AlexaFluor488, MCA497A488T, AbD Serotec, Raleigh, NC, USA), Ly-6G (neutrophils; anti-Ly6G-BV421, 562737, BD Biosciences), CD3 (T

(15) Targeting of cancer-associated fibroblasts enhances the efficacy of cancer chemotherapy by regulating the tumor microenvironmentMolecular Medicine ReportsMarch 1, 2016MINMIN LI, MEI LI, TAO YIN, HUASHAN SHI, YUAN WEN, BINGLAN ZHANG, MEIHUA CHEN, GUANGCHAO XU, KEXIN REN, YUQUAN WEIisothiocyanate (1:100; cat no. 553801; BD Biosciences), or rat anti-mouse F4/80-A488 (1:50; cat no. MCA497A488; Serotec, Bio-Rad Laboratories, Inc., Hercules, CA, USA) antibodies on ice for 30 min, followed by analysis

(16) CD73 Activity is Dispensable for the Polarization of M2 MacrophagesPloS oneJanuary 1, 2015Eichin D, Laurila JP, Jalkanen S, Salmi M.before stained for 30 min on ice with the following specific antibodies: anti-F4/80 FITC (rat IgG2b, #MCA497A488), anti-CD206 AF488 (rat IgG2a, #MCA2235A488) (both from AbD Serotec, Puchheim, Germany), anti-Ly6C FITC

(17) CD73 Activity is Dispensable for the Polarization of M2 MacrophagesPloS oneJanuary 1, 2015Eichin D, Laurila JP, Jalkanen S, Salmi M.before stained for 30 min on ice with the following specific antibodies: anti-F4/80 FITC (rat IgG2b, #MCA497A488), anti-CD206 AF488 (rat IgG2a, #MCA2235A488) (both from AbD Serotec, Puchheim, Germany), anti-Ly6C FITC

(18) Adjuvant cationic liposomes presenting MPL and IL-12 induce cell death, suppress tumor growth, and alter the cellular phenotype of tumors in a murine model of breast cancerMolecular pharmaceuticsOctober 6, 2014Meraz IM, Savage DJ, Segura-Ibarra V, Li J, Rhudy J, Gu J, Serda RE.53-6.7; 1:50), e-flour 570 Ki-67 (clone solA15; 1:100; eBioscience, San Diego, CA, USA); FITC F4/80 (MCA497A488;1:100); Alexa Fluor 647 CD204 (MCA1322; 1:100, AbD Serotec, Raleigh NC, USA); 33D1 (1:100, BD Biosciences,

(19) Adjuvant cationic liposomes presenting MPL and IL-12 induce cell death, suppress tumor growth, and alter the cellular phenotype of tumors in a murine model of breast cancerMolecular pharmaceuticsOctober 6, 2014Meraz IM, Savage DJ, Segura-Ibarra V, Li J, Rhudy J, Gu J, Serda RE.53-6.7; 1:50), e-flour 570 Ki-67 (clone solA15; 1:100; eBioscience, San Diego, CA, USA); FITC F4/80 (MCA497A488;1:100); Alexa Fluor 647 CD204 (MCA1322; 1:100, AbD Serotec, Raleigh NC, USA); 33D1 (1:100, BD Biosciences,

(20) Multivalent presentation of MPL by porous silicon microparticles favors T helper 1 polarization enhancing the anti-tumor efficacy of doxorubicin nanoliposomesPloS oneJanuary 1, 2014Meraz IM, Hearnden CH, Liu X, Yang M, Williams L, Savage DJ, Gu J, Rhudy JR, Yokoi K, Lavelle EC, et al.53-6.7; 1∶50), e-flour 570 ki67 (clone solA15; 1∶100; eBioscience, San Diego, CA, USA); FITC F4/80 (MCA497A488; 1∶100), Alexa Fluor 647 CD204 (MCA1322; 1∶50, AbD Serotec, Raleigh NC), Alexa Fluor 647 Gr-1 (clone

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RAT ANTI MOUSE F4/80:Alexa Fluor® 488

RAT ANTI MOUSE F4/80:Alexa Fluor® 488

RAT ANTI MOUSE F4/80:Alexa Fluor® 488

RAT ANTI MOUSE F4/80:Alexa Fluor® 488

RAT ANTI MOUSE F4/80:Alexa Fluor® 488

RAT ANTI MOUSE F4/80:Alexa Fluor® 488 from Bio-Rad (AbD Serotec - FAB)

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RAT ANTI MOUSE F4/80:Alexa Fluor® 488 from Bio-Rad (AbD Serotec - FAB)

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