Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC 3 (lane 1), LNCaP (lane 2), Hep G2 (lane 3) and THP-1 (Lane 4). The blots were probed with Anti-Androgen receptor Rabbit Polyclonal Antibody (Product # PA1-111B, 1-3 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 100 kDa band corresponding to Androgen receptor was observed across cell lines tested except Hep G2 and THP-1.Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by Pierce™ Power Blotter System (22834). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).