Western blot was performed using anti-Synapsin1 Polyclonal Antibody (Product # OPA1-04001) and 78 kDa band corresponding to Synapsin1 was observed across cell lines and tissues tested except Mouse and Rat Lung, Mouse Heart and Raji which are reported to be negative. Whole cell Extracts of (30 µg lysate) of IMR-32 (Lane 1), PC-12 (Lane 2), Raji (Lane 8), tissue extracts of Mouse Brain (Lane 3), Rat Brain (Lane 4), Mouse Lung (Lane 5), Rat Lung (Lane 6) and Mouse Heart (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1,000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).