Immunofluorescent analysis of Rab11B (green) in HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Rab11B rabbit polyclonal antibody (Product # PA1-775) at a dilution of 1:100 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731) at a dilution of 1:500 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.