Fig 1: Treatment with UB-ALT-EV changed the protein expression of inflammatory and astrocytic activation markers and in 5XFAD mice. Representative Western Blot (a) and quantifications for ratio phospho-NFATc1 vs. NFATc1 (b) and CaN (c). Values in bar graphs are adjusted to 100% for protein levels of the wild type (WT). Representative GFAP (Red) and Hoechst (Blue) positive staining in dentate gyrus, CA1 and CA3 hippocampal areas (d). Scale bars: 200 µm. Mean intensity of GFAP staining in dentate gyrus, CA1 region, and CA3 region (e). Representative Western Blot and quantification for GFAP (f). Values are the mean ± Standard error of the mean (SEM); (n = 3 for WT and Control groups and n = 4 for Mem and UB-ALT-EV groups. For WT vs. 5XFAD Control groups data was analyzed using a two-tail Student’s t-test, and for 5XFAD groups a standard one-way ANOVA followed by Tukey post-hoc analysis was performed. *p < 0.05; **p < 0.01 for WT vs. Control. #p < 0.05; ##p < 0.01 for Mem or UB-ALT-EV vs. Control.