beta-1 Adaptin Antibody PA5-66752 from Thermo Fisher Scientific

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WB

Western blot was performed using anti-beta-1 Adaptin Polyclonal Antibody (Product # PA5-66752) and a 110kDa band corresponding to beta-1 Adaptin was observed across all the cell lines and tissue lysates tested except Mouse Skeletal Muscle and Rat Skeletal Muscle which are reported negative for beta-1 Adaptin expression. An additional band which corresponds to an isoform of beta-1 Adaptin was detected at 65KDa in MCF7, MOLT-4, HepG2, Jurkat and LNCaP. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), MCF7 (Lane 2), MOLT-4 (Lane 3), Hep G2 (Lane 4), Jurkat (Lane 5), LNCaP (Lane 6), A549 (Lane 7), tissue extracts of Mouse Skeletal Muscle (Lane 8), Rat Skeletal Muscle (Lane 9), Mouse Brain (Lane 10) and Rat Brain (Lane 11) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:200 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
ICC/IF

Immunofluorescence analysis of beta-1 Adaptin was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta-1 Adaptin Polyclonal Antibody (Product # PA5-66752) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic, golgi and vesicular localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. .

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Thermo Fisher Scientific for
beta-1 Adaptin Antibody

Thermo Fisher Scientific's beta-1 Adaptin Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunocytochemistry, and Western Blot. The beta-1 Adaptin Antibody was generated using AP1B1 as the antigen and it reacts with Homo Sapiens, and Human.

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beta-1 Adaptin Antibody from Thermo Fisher Scientific

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beta-1 Adaptin Antibody from Thermo Fisher Scientific

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