MOUSE ANTI RAT CD163:Biotin MCA342B from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemMOUSE ANTI RAT CD163:Biotin
CompanyBio-Rad
Price $446.00Supplier Page View Company Product Page
Catalog NumberMCA342B
Quantity0.1 mg
ApplicationsFlow Cytometry
ConcentrationIgG concentration 0.1 mg/ml
Conjugate/TagBiotin
ReactivityRat
TargetCD163
IsotypeIgG1
CloneED2
HostMouse
ClonalityMonoclonal Antibody

Questions and Answers

Can this antibody be used to differentiate between M1 and M2 macrophages in rat tissues?

Yes, the Mouse Anti Rat CD163 antibody has been utilized in immunohistochemistry to label M2 macrophages, allowing for the differentiation between M1 and M2 phenotypes in rat tissues, as demonstrated in a study examining tendon repair processes ().

What type of sample preparation is recommended for using this antibody in immunohistochemistry?

For optimal results, samples should be fixed in 4% paraformaldehyde, permeabilized with 0.2% Triton X-100, and blocked with 5% goat serum before incubation with the Mouse Anti Rat CD163 antibody. This preparation was successfully employed in a study on Achilles tendon injuries ().

Is this antibody suitable for flow cytometry applications?

The Mouse Anti Rat CD163 antibody is specifically indicated for use in flow cytometry, which allows for the analysis of macrophage populations in rat samples, although specific experimental conditions are not detailed in the provided information.

What is the significance of using a biotin-conjugated antibody like this one in research?

Biotin-conjugated antibodies, such as the Mouse Anti Rat CD163, enhance detection sensitivity in various applications, including immunohistochemistry and flow cytometry, by allowing for the use of streptavidin-based detection systems, which can amplify the signal from the primary antibody.

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injuryJournal of neuroinflammationApril 30, 2019Hellenbrand DJ, Reichl KA, Travis BJ, Filipp ME, Khalil AS, Pulito DJ, Gavigan AV, Maginot ER, Arnold MT, Adler AG, et al.CD11b-Pacific Blue (Bio-Rad/AbD Serotec Cat# MCA275PB, RRID:AB_566459), CD163-Biotin (Bio-Rad/AbD Serotec Cat# MCA342B, RRID:AB_2074559), CD68-PE (Bio-Rad/AbD Serotec Cat# MCA341PE, RRID:AB_324585), CD86-Alexafluor 647

(2) Changes in macrophage phenotype and induction of epithelial-to-mesenchymal transition genes following acute Achilles tenotomy and repairJournal of Orthopaedic ResearchJuly 1, 2014Kristoffer B. Sugg, Jovan Lubardic, Jonathan P. Gumucio, Christopher L. Mendiasantibodies against rabbit anti-CCR7 (NB110-55680, Novus Biologicals, Littleton, CO) and mouse anti-CD163 (MCA342B, AbD Serotec, Raleigh, NC) to label M1 and M2 macrophages, respectively 19; 20. AlexaFluor 555 (AF555,

(3) Immunosuppressive Treatment Protects Against Angiotensin II-Induced Renal DamageThe American Journal of PathologyJuly 24, 2002Dominik N. Muller, Erdenechimeg Shagdarsuren, Joon-Keun Park, Ralf Dechend, Eero Mervaala, Franziska Hampich, Anette Fiebeler, Xinsheng Ju, Piet Finckenberg, Jürgen Theuer, Christiane Viedt, Joerg Kreuzer, Harald Heidecke, Hermann Haller, Martin Zenke, Friedrich C. Luft

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MOUSE ANTI RAT CD163:Biotin

MOUSE ANTI RAT CD163:Biotin

MOUSE ANTI RAT CD163:Biotin

MOUSE ANTI RAT CD163:Biotin

MOUSE ANTI RAT CD163:Biotin

MOUSE ANTI RAT CD163:Biotin from Bio-Rad (Formerly AbD Serotec)

Description

Questions and Answers

Bio-Rad

Citations (3)

Reviews

MOUSE ANTI RAT CD163:Biotin from Bio-Rad (Formerly AbD Serotec)

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