Immunofluorescence staining of SIRT2 in transfected SAOS2 cells using Product # PA3-200.

Western blot analysis was performed on whole cell, tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Brain (Lane 2), MCF 7 (Lane 3), and HeLa (Lane 4). The blots were probed with Anti-SIRT2 Rabbit Polyclonal Antibody (Product # PA3-200, 1:250 dilution) and detected by chemiluminescence using Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 30 kDa band corresponding to SIRT2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).