Anti-PCNA Rabbit Monoclonal Antibody M00125-1 from BosterBio | Biocompare.com

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Anti-PCNA Rabbit Monoclonal Antibody

Western blot analysis of PCNA using anti-PCNA antibody (M00125-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U-937 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: rat RAW264.7 tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCNA antigen affinity purified monoclonal antibody (Catalog # M00125-1) at 1:3000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCNA at approximately 36 kDa. The expected band size for PCNA is at 29 kDa.
Anti-PCNA Rabbit Monoclonal Antibody

Anti-PCNA Rabbit Monoclonal Antibody
Anti-PCNA Rabbit Monoclonal Antibody

IF analysis of PCNA using anti-PCNA antibody (M00125-1) and anti-Beta Tubulin antibody (M01857-3).
PCNA was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:100 with rabbit anti-PCNA Antibody (M00125-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog#BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Anti-PCNA Rabbit Monoclonal Antibody

Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)

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BosterBio for
Anti-PCNA Rabbit Monoclonal Antibody

The Anti-PCNA Rabbit Monoclonal Antibody from BosterBio is a Rabbit Monoclonal antibody to PCNA, and proliferating cell nuclear antigen. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Anti-PCNA Rabbit Monoclonal Antibody has been shown to work in the following applications: Precipitation, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blot.

Description

Boster Bio Anti-PCNA Rabbit Monoclonal Antibody catalog # M00125-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat

Product Specs
ItemAnti-PCNA Rabbit Monoclonal Antibody
CompanyBosterBio
Price $370.00Supplier Page View Company Product Page
Catalog NumberM00125-1
Quantity100 µl/vial
ApplicationsWB,IHC,ICC,IF,IP,Flow Cytometry
FormatRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA., Liquid
ReactivityHuman,Mouse,Rat
HostRabbit
ImmunogenA synthesized peptide derived from human PCNA
NCBI Gene AliasesATLD2
ClonalityMonoclonal
Molecular Weight28769 MW, Observed: 36 kDa
Calculated: 28769 MW
Gene NamePCNA
PurityAffinity-chromatography
Research AreaCell Biology,Cell Cycle,Cell Division,Cell Type Markers,Chromatin Binding Proteins,DNA/RNA,DNA/RNA Binding,DNA Synthesis,Epigenetics and Nuclear Signaling,Tags & Cell Markers,Tumor Antigens,Tumor Biomarkers
CloneDO-16
Concentration0.5mg/ml
StorageStore at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles
IsotypeIgG
TargetCytochrome P450 1A
Antibody TypePrimary Antibodies

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BosterBio

BosterBio

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United States

Phone: (888) 466-3604
Fax: (925)215-2184

Email: support@bosterbio.com

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Website: www.bosterbio.com

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