mTOR Antibody PA1-518 from Thermo Fisher Scientific

Product Specs
ItemmTOR Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-518
NCBI Gene Aliasesflat, RAFT1, SKS, FRAP1, FRAP2, Frap1, RAPT1, FRAP, 2610315D21Rik
TargetmTOR
Conjugate/TagUnconjugated
FormatPBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
Quantity100 µg
ApplicationsImmunocytochemistry, Immunoprecipitation, Western Blot
ClonalityPolyclonal
ReactivityHuman, Mouse, Non-human primate, Rat
HostRabbit
NCBI Full Gene Namemechanistic target of rapamycin kinase
ImmunogenImmunogen corresponds to amino acids 1223-1290 of human mTOR
IsotypeIgG
Concentration1 mg/mL
Storage Temperature-20°C
PurityProtein G
Antibody TypePrimary
Storage-20°C

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Spatial dynamics of mTOR pathway activity during bovine embryo developmentFrontiers in Cell and Developmental BiologyFebruary 25, 2026Francieli P. Berling, Viviane B. G. Bacaro, Ricardo I. de Paschoal, Marcella P. Milazzotto, Marcelo D. GoissisUnited States), anti-phospho-S6 (1,875 mg/mL, D68F8, Cell Signaling Technology, United States), or mTOR (PA1-518, 25 μg/mL ThermoFisher, United States), followed by washes and incubation with secondary antibodies

(2) Adipose-derived stem cells promote the repair of chemotherapy-induced premature ovarian failure by inhibiting granulosa cells apoptosis and senescenceStem Cell Research & TherapyApril 11, 2023Guihai Ai, Meng Meng, Jing Guo, Caixia Li, Jihui Zhu, Li Liu, Biting Liu, Wenhan Yang, Xiaowen Shao, Zhongping Cheng, et al.AKT (1:100, Thermo Fisher Scientific, MA5-14,916), anti-rat mTOR (1:100, Thermo Fisher Scientific, PA1-518), anti-rat FOXO3a (1:100, Thermo Fisher Scientific, MA5-14,932) and anti-rat RPS6 (1:100, Thermo Fisher

(3) PTPN14 promotes gastric cancer progression by PI3KA/AKT/mTOR pathwayCell death & diseaseMarch 10, 2023Li H, Guan B, Liu S, Liu H, Song L, Zhang G, Zhao R, Zhou C, Gao P.(Invitrogen, CA, USA, 44–609G), p-AKT-S473 (Invitrogen, CA, USA, PA5–104445), mTOR (Invitrogen, CA, USA, PA1–518), p-mTOR-S2448 (Invitrogen, CA, USA, 44–1125G), CDK4 (Cell Signaling Technology, Boston, USA, 12790),

(4) Partial Synthetic PPARƳ Derivative Ameliorates Aorta Injury in Experimental Diabetic Rats Mediated by Activation of miR-126-5p Pi3k/AKT/PDK 1/mTOR ExpressionPharmaceuticals (Basel, Switzerland)September 22, 2022Ahmed YM, Orfali R, Abdelwahab NS, Hassan HM, Rateb ME, AboulMagd AM.

(5) Liposomes Loaded with Everolimus and Coated with Hyaluronic Acid: A Promising Approach for Lung FibrosisInternational journal of molecular sciencesJuly 20, 2021Pandolfi L, Marengo A, Japiassu KB, Frangipane V, Tsapis N, Bincoletto V, Codullo V, Bozzini S, Morosini M, Lettieri S, et al.and incubated with the primary Ab (1:1000 in TBST + 5% BSA; overnight at 4 °C): anti-mTOR (1:1000) (PA1518—abcam), anti-p-mTOR(Ser2448) (1:1000) (PA585736—abcam), and anti-β-Actin (1:5000) (MA1-140—Thermo Fisher Scientific

(6) Development of thermosensitive resiquimod-loaded liposomes for enhanced cancer immunotherapyJournal of controlled release : official journal of the Controlled Release SocietyFebruary 10, 2021Zhang H, Tang WL, Kheirolomoom A, Fite BZ, Wu B, Lau K, Baikoghli M, Raie MN, Tumbale SK, Foiret J, et al.CD8 (1:500 dilution; 14–0808, eBiosciences) was used as CD8 marker. Anti-mouse CD68 (1:700 dilution; PA1518, Boster) was used to stain macrophages. Anti-mouse OX40 antibody (1:2000 dilution; ab229021, abcam)

(7) Imaging endogenous macrophage iron deposits reveals a metabolic biomarker of polarized tumor macrophage infiltration and response to CSF1R breast cancer immunotherapyScientific ReportsDecember 1, 2019Avigdor Leftin, Nir Ben-Chetrit, Johanna A. Joyce, Jason A. Koutchernuclear-fast red counter-staining for 30 minutes. The immunofluorescent detection of CD68 (Boster, cat# PA1518, 5 ug/ml, 5 hours) was performed using standard Discovery XT processor protocols and reagents (Ventana Medical Systems

(8) The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune SuppressionCancer cellAugust 12, 2019Su W, Han HH, Wang Y, Zhang B, Zhou B, Cheng Y, Rumandla A, Gurrapu S, Chakraborty G, Su J, et al.(Abcam, cat# ab133357, 1 μg/ml); anti-CCL2 (Invitrogen, MA5–17040, 1:200); anti-CD68 (Boster, cat# PA1518, 5 μg/ml); anti-CD8 (Cell Signaling, cat# 98941, 2.4 μg/ml); anti-CD31 (Dianova, cat# DIA-310, 1 μg/ml);

(9) Altered Processing of β-Amyloid in SH-SY5Y Cells Induced by Model Senescent MicrogliaACS chemical neuroscienceDecember 19, 2018Angelova DM, Brown DR.

(10) Effect and molecular mechanism of mTOR inhibitor rapamycin on temozolomide-induced autophagic death of U251 glioma cellsOncology lettersFebruary 1, 2018Li B, Zhou C, Yi L, Xu L, Xu M.

(11) Bilirubin activates transcription of HIF-1α in human proximal tubular cells cultured in the physiologic oxygen contentJournal of Korean medical scienceSeptember 1, 2014Kim SG, Ahn SY, Lee ES, Kim S, Na KY, Chae DW, Chin HJ.(#9271), anti-phospho-mTOR (#5536) (Cell Signaling Technology, MA, USA), anti-mTOR (Thermo Scientific Pierce, PA1-518, IL, USA), anti-RAPTOR (Abcam ab5454, CA, USA), anti-actin (sc-1616), anti-NOX 4 (sc-20141), anti-p22phox

(12) Optimized method for rapid protein electroblottingNature MethodsOctober 1, 2013Greg Kilmer, Brian Webb, Boguslawa R Dworecki, Eric Hommema, Steve Shiflett, Priya Rangarajtank Overnight thirds. The top sections were probed with either Thermo Scientific™ anti-mTOR Antibody (Product #PA1-518) or Thermo Scientific™ anti- Figure 3 | Western blot comparison of low–, medium– and high–molecular weight

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mTOR Antibody from Thermo Fisher Scientific

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