Western blot analysis was performed using whole cell extract (30 µg) of RAW 264.7 (Lane 1), PC-12 (Lane 2), PC-12 treated with Retinoic acid 1uM for 3 days (Lane 3), SH-SY5Y (Lane 4) and SH-SY5Y treated with Retinoic acid 1uM for 3 days (Lane 5). The blots were probed with Anti- Dynamin I Rabbit Polyclonal (Product # PA1-660, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 97 kDa band corresponding to Dynamin I was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800).) Resolved proteins were then transferred onto a nitrocellulose membrane by Pierce™ Power Blotter System (22834). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).