CD45RO Antibody orb385702 from Biorbyt

Description

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas

Product Specs
ItemCD45RO Antibody
CompanyBiorbyt
Price $370.00Supplier Page
Catalog Numberorb385702
Quantity20 ug, 100 ug
ApplicationsFACS, IHC-P
Conjugate/TagUnconjugated
ReactivityHuman
TargetCD45RO
IsotypeMouse IgG2a, kappa
CloneT200/797
HostMouse
ImmunogenHuman T lymphocytes were used as immunogen for this CD45RO antibody
NCBI Full Gene Nameprotein tyrosine phosphatase receptor type C
NCBI Gene AliasesB220, CD45, CD45R, GP180, IMD105, L-CA, LCA, LY5, T200
ClonalityMonoclonal
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Research AreaEpigenetics, Infectious Diseases

Specifications/Features

Application Notes: Due to variation in protocol and secondary antibody used, the CD45RO antibody may need to be titered for optimal performance.1. FFPE staining requires 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Application Dilutions: Flow cytometry: 0.5-1ug/10^6 cells,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT

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