Fig 1: RPH1 is genetically involved in the TORC1 pathway. (A) The indicated strains were spotted on YPD plates with low dose of rapamycin. Pictures were taken after grown 2 or 4 days. (B) Tet-off system derived yeast strains were spotted on plates with the indicated doses of rapamycin in the presence of doxycycline. (C) Log-phase cells were cultured under rapamycin treatment, and were isolated at the indicated times and subjected to immunoblot with different antibodies. G6PDH served as a loading control. Asterisk indicates a non-specific band. (D) The phosphorylated Rps6 levels relative to total Rps6 levels in panel C were plotted with the indicated time points and then normalized to the time 0 point of individual strain by densitometric analysis using ImageJ software. The error bars represent mean ± SD from three independent biological repeats. t-test, **P < 0.01.