The PKC-alpha (Ser-657/Tyr-658), phospho-specific Antibody from MyBioSource.com is a Rabbit Polyclonal antibody to PKC alpha, PKCA, PRKCA, and protein kinase C alpha. This antibody recognizes Human, Rat, and Rabbit antigen. The PKC-alpha (Ser-657/Tyr-658), phospho-specific Antibody has been shown to work in the following applications: ELISA, Immunoprecipitation, and Western Blot.
Description
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (alpha, beta-I, beta-II, and gamma) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, Fc-epsilon-RI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKC-beta-I and PKC-alpha, respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases