Fig 2: Absence of inducible nitric oxide synthase (iNOS)+ M1 macrophages from the lens capsule–associated immune cell population at day 18 (D18) and D35 after induction of experimental autoimmune uveitis (EAU). A–C: Whole eye cryosections from mice with EAU at D18 (Aa–c, Ba–c, and Ca–c) and D35 (Ad–f, Bd–f, and Cd–f) after induction were co-immunolabeled for iNOS (green) and CD11b (white), and confocal microscopy images were acquired along the anterior (Aa–f), equatorial (Ba–f), and posterior (Ca–f) surfaces of the lens capsule. Dotted lines delineate the surfaces of the lens capsule. All images are 2.5-μm projections. Images shown are representative of at least three independent studies. Scale bars = 20 μm (A–C). AqH, aqueous humor; VH, vitreous humor.

Fig 3: Immune cells present in the vitreous during active experimental autoimmune uveitis (EAU) inflammation express inducible nitric oxide synthase (iNOS). A–C: Whole eye cryosections at day 18 after induction of EAU were co-immunolabeled for iNOS (green; A), CD11b (white; B), and CD3 (red; C). D: An overlay image of iNOS, CD11b, and CD3. Confocal microscopy images were captured in the vitreous. Images shown are single optical plane and representative of at least three independent studies. Scale bar = 20 μm (A–D).