Fig 1: CAPS affects the phosphorylation of PLK1 by interacting with MYPT1. (A) Venn diagram showing 27 genes found to interact with CAPS, among which two genes were involved in the G2/M phase transition. (B,C) Co-IP assay showing the interaction of CAPS with MYPT1, but not with RPS27A. (D) Phosphorylation at different sites of MYPT1 in LN229 and GBM1 cells expressing control shRNA, shCAPS-1, or shCAPS-2, as detected by western blotting. (E) LN229 and GBM1 cells expressing control shRNA or shCAPS-1 were synchronized by a double thymidine block. The levels of p-PLK1 and t-PLK1 were analyzed at different time points after release. (F) Pearson correlation analysis of CAPS and p-PLK1 in 172 glioma samples. (G) Total and phosphorylated PLK1 and MYPT1 were detected by western blotting in LN229 and GBM1 cells expressing control shRNA, shCAPS, or shCAPS+MYPT1 S507E. (H) Proliferation and (I) cell cycle analysis of LN229 and GBM1 cells expressing control shRNA, shCAPS, or shCAPS+MYPT1 S507E. ***p < 0.001.
Supplier Page from Cell Signaling Technology for MYPT1 Antibody Sampler Kit