Fig 1: CAPS affects the phosphorylation of PLK1 by interacting with MYPT1. (A) Venn diagram showing 27 genes found to interact with CAPS, among which two genes were involved in the G2/M phase transition. (B,C) Co‐IP assay showing the interaction of CAPS with MYPT1, but not with RPS27A. (D) Phosphorylation at different sites of MYPT1 in LN229 and GBM1 cells expressing control shRNA, shCAPS‐1, or shCAPS‐2, as detected by western blotting. (E) LN229 and GBM1 cells expressing control shRNA or shCAPS‐1 were synchronized by a double thymidine block. The levels of p‐PLK1 and t‐PLK1 were analyzed at different time points after release. (F) Pearson correlation analysis of CAPS and p‐PLK1 in 172 glioma samples. (G) Total and phosphorylated PLK1 and MYPT1 were detected by western blotting in LN229 and GBM1 cells expressing control shRNA, shCAPS, or shCAPS+MYPT1 S507E. (H) Proliferation and (I) cell cycle analysis of LN229 and GBM1 cells expressing control shRNA, shCAPS, or shCAPS+MYPT1 S507E. ***p < 0.001.
from Cell Signaling Technology for MYPT1 Antibody Sampler Kit