Fig 1: Increased mTORC1 signaling intermediates and persistent activation of mTORC1 signaling pathway in RPE of Cryba1 KO mice even after fasting. (A) Representative western blot of p‐mTOR (Ser2448) and p70S6K (Thr421/Ser424) in RPE lysates from fed and 24‐h‐fasted Cryba1 fl/fl or Cryba1 KO mice (n = 4). (B) and (C) show densitometric quantification for p‐mTOR and p‐70S6K, respectively. (D) Representative western blots for LAMTOR2, LAMTOR3, RagA, and RagB expression and (E) densitometric quantification of the western blot data for RPE lysates from fed and 24‐h‐fasted Cryba1 fl/fl or Cryba1 KO mice (n = 5). Actin was used as internal control in all blots. Values were normalized to that of fed Cryba1 fl/fl samples which relative expression was as 1. All data are expressed as mean ± SEM. *P < 0.05, **P < 0.01.