Fig 1: Increased mTORC1 signaling intermediates and persistent activation of mTORC1 signaling pathway in RPE of Cryba1 KO mice even after fasting. (A) Representative western blot of p-mTOR (Ser2448) and p70S6K (Thr421/Ser424) in RPE lysates from fed and 24-h-fasted Cryba1 fl/fl or Cryba1 KO mice (n = 4). (B) and (C) show densitometric quantification for p-mTOR and p-70S6K, respectively. (D) Representative western blots for LAMTOR2, LAMTOR3, RagA, and RagB expression and (E) densitometric quantification of the western blot data for RPE lysates from fed and 24-h-fasted Cryba1 fl/fl or Cryba1 KO mice (n = 5). Actin was used as internal control in all blots. Values were normalized to that of fed Cryba1 fl/fl samples which relative expression was as 1. All data are expressed as mean ± SEM. *P < 0.05, **P < 0.01.