Fig 2: DR6 functions through its association with KIF11 and TRAF4. (A) Overexpression of KIF11 or TRAF4 reversed the DR6 knockdown‐mediated reduction in SKOV3 cell migration. Scale bar = 200μm. (B) Knocking down of KIF11 or TRAF4 reversed the DR6 overexpression‐mediated increase in SKOV3 cell migration. Scale bar = 200μm. (C) Western blot analysis of indicated protein expression in SKOV3 cells. Data are presented as mean ± SD (n = 8). **P < 0.01 (Student's t test).

Fig 3: The correlation between DR6 and KIF11/TRAF4 expression in OVCA. (A) The correlation between DR6 and KIF11 expression in OVCA tissues (n = 50). (B) The correlation between DR6 and TRAF4 expression in OVCA tissues (n = 50).

Fig 4: SRC-3 upregulated the expression of TRAF4 to enhance the growth, migration, invasion and stemness of ovarian cancer cells. A Western blot detected the expression of SRC-3, TRAF4, PI3K, AKT, p-AKT and p-PI3K in cells after knocking down SRC-3 and overexpressing TRAF4. B MTT method detected the proliferation of CAOV-3 and SKOV3 cells. C and D The effect of Transwell analysis on cell migration and invasion. E The cell spheroidization test detected the number of spheroids of cells. F and G The expression of stem cell factors was analyzed by qRT-PCR and western blot. n = 3. *P < 0.05, **P < 0.01, *** P < 0.001

Fig 5: SRC-3 and TRAF4 were upregulated in ovarian cancer cells. A The mRNA level of SRC-3 and TRAF4 were detected by qRT-PCR in ovarian cancer cells and normal ovarian epithelial cells. B The protein levels of SRC-3 and TRAF4 were detected by western blot in CAOV-3 and SKOV3 cells and normal ovarian epithelial cells. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001