DDX5 (D15E10) XP ® Rabbit mAb 9877T from Cell Signaling Technology

Product Specs
ItemDDX5 (D15E10) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $155.00 Inquire
Catalog Number9877T
Molecular Weight68
Research AreaTranslational Control
ReactivityHu, Ms, Rt, Mk
ClonalityMonoclonal
NCBI Gene AliasesG17P1, HLR1, HUMP68, p68
NCBI Full Gene NameDEAD-box helicase 5
ApplicationsWB, ICC, IF, IP
HostRabbit
IsotypeIgG
TargetDDX5
Quantity20 µl

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Cell Signaling Technology

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Danvers, Massachusetts 01923

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Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) HDAC5 -encoded Microprotein NISM Mediates Nucleolar Formation and Ribosomal RNA SynthesisbioRxivFebruary 25, 2026Kevin Cao, Dat Ha, Jesse Hulahan, Lilianna Houston, Gregory Tong, Jiayi Weng, Natasha Huey, Hannah E. DeMerit, Pedro Ortega, Rémi Buisson, et al.were incubated with rabbit monoclonal anti-DHX9 (Cell Signaling, 71286) and anti-DDX5 (Cell Signaling, 9877) diluted 1:500 in blocking buffer. FLAG immunoblots were incubated with mouse monoclonal anti-FLAG (Sigma-Aldrich,

(2) The lincRNA Pantr1 is a FOXG1 target gene conferring site-specific chromatin binding of FOXG1Nucleic Acids ResJune 23, 2025Fabian Gather, Tudor Rauleac, Ipek Akol, Ganeshkumar Arumugam, Camila L Fullio, Teresa Müller, Dimitrios Kleidonas, Ruth Geiss-Friedlander, Andre Fischer, Andreas Vlachos, et al.antibodies were used: anti-FOXG1 (61 211, Active Motif), anti-NEUROD1 (ab109224, Abcam), anti-DDX5 (9877, Cell Signaling, USA), anti-PURB (ab130999, Abcam) diluted in 5% BSA in TBST (TBS with 0.1% Tween-20

(3) LncRNA JINR1 regulates miR-216b-5p/ GRP78 and miR-1-3p/ DDX5 axis to promote JEV infection and cell deathJournal of VirologyMay 20, 2025Shraddha Tripathi, Suryansh Sengar, Anirban Basu, Vivek Sharmaantibodies were used: GRP78 (1:5,000, #ab21685, Abcam), Cleaved PARP (1:2,000, #5625, CST), DDX5 (1:3,000, #9877, CST), horseradish peroxidase (HRP)-conjugated anti-rabbit (#PI 2000, Vector Laboratories), and anti-mouse

(4) AURKAIP1 actuates tumor progression through stabilizing DDX5 in triple negative breast cancerCell death & diseaseDecember 1, 2023Tian W, Tang Y, Luo Y, Xie J, Zheng S, Zou Y, Huang X, Wu L, Zhang J, Sun Y, et al.Primary antibodies were listed as follows: anti-AURKAIP1(PA5-50356/PA5-56869, Invitrogen), anti-DDX5 (#9877, CST), anti-Flag (66008-4-Ig/ 20543-1-AP, Proteintech), anti-HA (51064-2-AP/ 66006-2-Ig, Proteintech),

(5) DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancerCell death & diseaseJanuary 2, 2023Zhao G, Wang Q, Zhang Y, Gu R, Liu M, Li Q, Zhang J, Yuan H, Feng T, Ou D, et al.antibodies: anti-DDX17 (Santa Cruz Biotechnology, sc-398168), anti-DDX5 (Cell Signaling Technology, CST, #9877), anti-CYBRD1 (Abcam, ab66048), anti-E-cadherin (CST, #3195), anti-Claudin-1 (CST, #13255), anti-Vimentin

(6) Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cellsNature communicationsMay 20, 2022Zhang J, Gan Y, Li H, Yin J, He X, Lin L, Xu S, Fang Z, Kim BW, Gao L, et al.antibodies were purchased from Cell Signaling Technology as below: DDX5 (D15E10,1:1000) XP® Rabbit mAb(#9877,1:1000); Stathmin Antibody(#3352,1:1000); Mcl-1 (D5V5L) Rabbit mAb(#39224,1:1000); Anti-mouse IgG, HRP-linked

(7) WDR82-binding long noncoding RNA lncEry controls mouse erythroid differentiation and maturationThe Journal of experimental medicineApril 4, 2022Yang S, Sun G, Wu P, Chen C, Kuang Y, Liu L, Zheng Z, He Y, Gu Q, Lu T, et al.H3K27ac (ab6002; 1:200 for Cut&Tag) from Abcam; Wdr82 (99715; 1:1,000 for WB; 1:100 for Cut&Tag), DDX5 (9877T; 1:1,000 for WB), and RBBP5 (13171S; 1:200 for ChIP) from Cell Signaling Technology; PCBP2 (NBP2-19715;

(8) TWIST1 induces phenotypic switching of vascular smooth muscle cells by downregulating p68 and microRNA-143/145FEBS open bioMarch 1, 2021Zhang J, Guo JR, Wu XL, Wang X, Zhu ZM, Wang Y, Gu X, Fan Y.(kDa) TWIST1,CST,46702,rabbit,26 β‐actin,Boster,BM0627,mouse,42 SMMHC,PROTEINTECH,21404‐1‐AP,rabbit,200 SMA,ABCAM,Ab7817,mouse,42 calponin,PROTEINTECH,13939‐1‐AP,rabbit,33 tagln,PROTEINTECH,10493‐1‐AP,rabbit,22 GAPDH,CST,5174S,rabbit,37 p68,CST,9877,rabbit,68 Antibodies for immunoblot analyses

(9) Circular RNA circEGFR regulates tumor progression via the miR-106a-5p/DDX5 axis in colorectal cancerBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicasJanuary 1, 2021Fu P, Lin L, Zhou H, Zhao S, Jie Z.membranes. Then, the membranes were incubated overnight at 4°C with AKT (#2920), p-AKT (#4051), DDX5 (#9877) antibodies (1:1000, Cell Signaling Technology, USA), and β-actin antibodies (sc-8432) (1:1000, Santa Cruz

(10) Zika Infection Disrupts Proteins Involved in the Neurosensory SystemFrontiers in Cell and Developmental BiologyJuly 29, 2020Kathleen K. M. Glover, Ali Zahedi-Amiri, Ying Lao, Victor Spicer, Thomas Klonisch, Kevin M. CoombsBAD (Cell Signaling, Cat. # 9292), NF-κB2 (Cell Signaling, Cat. # 4882), DDX5 (Cell Signaling, Cat. # 9877), and EIF4A1 (Cell Signaling, Cat. # 9292). Primary antibody reactivity was detected with HRP-conjugated

(11) LINC01116 promotes tumor proliferation and neutrophil recruitment via DDX5-mediated regulation of IL-1β in glioma cellCell death & diseaseMay 1, 2020Wang T, Cao L, Dong X, Wu F, De W, Huang L, Wan Q.primary antibodies were used: anti-IL-1β (16806-1-AP, 1:100, Proteintech, WuHan,China) and anti-DDX5 (#9877, 1:1000, Cell Signaling technology, MA, USA). For ELISA, IL-1β in culture supernatants of glioma cells

(12) Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of TranscriptionCellJune 27, 2019Xiao R, Chen JY, Liang Z, Luo D, Chen G, Lu ZJ, Chen Y, Zhou B, Li H, Du X, et al.anti-DDX3X,Zen BioScience,201041; RRID: AB_2616548 Rabbit monoclonal anti-DDX5,Cell Signaling Technology,9877S; RRID: AB_10891054 Rabbit polyclonal anti-DDX59,Bethyl,A303-028A; RRID: AB_10755233 Rabbit polyclonal

(13) FOXC1-induced non-canonical WNT5A-MMP7 signaling regulates invasiveness in triple-negative breast cancerOncogeneMarch 1, 2018Han B, Zhou B, Qu Y, Gao B, Xu Y, Chung S, Tanaka H, Yang W, Giuliano AE, Cui X.(1:500, sc-10042, Santa Cruz), TRIM28 (1:1000, #4123, Cell Signaling Technology), and DDX5 (1:1000, #9877, Cell Signaling Technology). I, real-time PCR analysis of WNT5A mRNA expression in MDA-MB-231 cells

(14) TUT‐DIS3L2 is a mammalian surveillance pathway for aberrant structured non‐coding RNAsThe EMBO JournalOctober 17, 2016Dmytro Ustianenko, Josef Pasulka, Zuzana Feketova, Lukas Bednarik, Dagmar Zigackova, Andrea Fortova, Mihaela Zavolan, Stepanka VanacovaTechnologies, cat. no. 6571; anti‐DDX5 (D15E10) XP rabbit monoclonal Ab, Cell Signalling Technologies, cat. no. 9877; anti‐rabbit IgG HRP polyclonal secondary Ab, Promega, cat. no. W4011; anti‐mouse IgG HRP polyclonal

(15) Regulation of MicroRNA 183 by Cyclooxygenase 2 in Liver Is DEAD-Box Helicase p68 (DDX5) Dependent: Role in Insulin SignalingMolecular and cellular biologyJuly 1, 2015Motiño O, Francés DE, Mayoral R, Castro-Sánchez L, Fernández-Velasco M, Boscá L, García-Monzón C, Brea R, Casado M, Agra N, et al.respectively); DGCR8 (Proteintech 10996-1-AP); DDX5, Drosha, p-AKT (Ser473), AKT, and IR (Cell Signaling Technology 9877, 3364, 4060, 9272, and 3020, respectively); DDX17 (Abcam ab70184); and Dicer (dicer 1, RNase type III;

(16) Oncomorphic TP53 Mutations in Gynecologic Cancers Lose the Normal Protein:Protein Interactions with the microRNA Microprocessing ComplexJournal of Cancer TherapyJune 1, 2014Pavla Brachova, Samuel R. Mueting, Eric J. Devor, Kimberly K. LeslieBeads (eBioscience). Beads were prepared according to the manufacturer’s protocol, by binding DDX5 (#9877, Cell Signaling), p53 (#2527, Cell Signaling), or IgG (#2729, Cell Signaling) control antibodies. UCI-107

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DDX5 (D15E10) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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DDX5 (D15E10) XP ® Rabbit mAb from Cell Signaling Technology

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