Fig 2: Effects of Wnt/β-catenin signaling pathway on proliferation and activation of HSC-T6 cells. A The protein expression of Wnt3a, β-catenin and p-GSK3β from rat liver tissues was detected by western blotting. B The cell viability of HSC-T6 cells was measured by CCK-8 analysis. C The cell cycle was detected by flow cytometry analysis of HSC-T6 cellsp. D The protein expression of α-SMA, Collagen I, cyclin-D1, c-Myc, MMP7, Wnt3a, β-catenin and p-GSK3β in HSC-T6 cells was detected by western blotting. Data were presented as the mean ± SD obtained from three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001

Fig 3: Knockdown of BRG1 inhibits tubular senescence and the activation of Wnt/β-catenin signalling pathway in UUO mice(A–D) Western blot analysis demonstrated that knockdown of BRG1 attenuated renal expression of p16INK4a, p21, and p19ARF proteins induced by UUO. Representative Western blot (A) and quantification of protein levels of renal p16INK4a (B), p21 (C), and p19ARF (D) are presented. (E) Representative staining micrographs showed renal SA-β-gal activity, p16INK4a, and TGF-β1 expression in different groups as indicated. Frozen kidney sections were stained for SA-β-gal activity, paraffin-embedded kidney sections were immunostained with antibodies against p16INK4a or TGF-β1. Scale bar, 50 μm. (F–J) Western blot analysis showed that renal expression of active-β-catenin, MMP-7, snail-1, and PAI-1 were significantly inhibited by knockdown of BRG1 in UUO mice. Representative western blot (F) and quantitative data (G–J) are presented. **P<0.01, ***P<0.001 versus sham group; #P<0.05, ##P<0.01, ###P<0.001 versus UUO alone (n=6).

Fig 4: miR-139-5p inhibited colon cancer cell invasion. (A) HCT116 and LoVo cells were transfected with miR-139-5p or NC mimics for 48 h, and subsequently cell invasion was determined using a transwell invasion assay (original magnification, ×200). (B) The protein expression levels of MMP7 and MMP9 were evaluated by western blot assays in HCT116 and LoVo cells transfected with miR-139-5p or NC mimics. **P<0.01 vs. blank or NC group. miR, microRNA; NC, negative control; MMP, matrix metalloproteinase.

Fig 5: (A) Expression of PAX5, p53, GADD45G, CDC2, TIMP2, MMP2 and MMP9 was evaluated by semi‐quantitative PCR in vector‐ and PAX5‐transfected A549 and H1975 cells. GAPDH was used as an internal control, *P < 0.05. (B) The mRNA expression of β‐catenin, cyclin D1, C‐Myc and MMP7 in vector‐ and PAX5‐transfected A549 cells by qRT‐PCR. β‐actin was used as a control. **P < 0.01, ***P < 0.001. (C) Effects of ectopic expression of PAX5 on protein expression of β‐catenin, active β‐catenin, cyclin D1, C‐Myc and GADD45G using western blot. GAPDH was used as an internal control. (D) Schematic diagram for the mechanisms of anti‐tumorigenesis functions of PAX5 deriving from semi‐quantitative PCR and western blot.