Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb 9649T from Cell Signaling Technology

Product Specs
ItemAcetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb
CompanyCell Signaling Technology
Price $163.00 Inquire
Catalog Number9649T
Quantity20 µl
ApplicationsW, IP, IHC-P, IF-IC, FC-FP, ChIP, ChIP-seq, C&R, C&T
ReactivityH, M, R, Mk, Z
TargetH3 (Lys9) acetylate
IsotypeIgG
HostRabbit
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight17

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Cell Signaling Technology

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Danvers, Massachusetts 01923

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Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Discovery of a CI-994 derivative as a dual modulator of class I histone deacetylases and Wnt/β-catenin signaling for Alzheimer's disease therapyNeurotherapeuticsJuly 27, 2026Wenyan Lu, Thomas R. Caulfield, Eunmi Lee, Suren Jeevaratnam, Ni Wang, Guojun Bu, Takahisa Kanekiyo, Yonghe Liand their dilutions used in this study are as follows: acetyl-histone H3 (Cell Signaling Technology, 9649S, 1:1000), histone H3 (Cell Signaling Technology, 4499S, 1:1000), β-catenin (BD Biosciences, 610154,

(2) Pharmacological chromatin remodeling enhances response to estrogen therapy in ER + breast cancerMol OncolJuly 17, 2026Anneka L. Johnson Thomas, Barbara Karakyriakou, Patricia C. Muskus, Alyssa M. Roberts, Justin Rendleman, Alexa Warren, Hanxu Lu, Kimberly A. Birmingham, Malone K. Friedman, Abigail E. Goen, et al.(Cell Signaling Technology, Danvers, MA, USA, #8173), acetyl‐histone H3 Lys9 (Cell Signaling Technology #9649), IRS‐1 (Cell Signaling Technology #3407), full‐length and cleaved PARP (Cell Signaling Technology #9532),

(3) Endothelial epigenetic senescence driven microglial activation mediates cardio-retinal neuroinflammation in heart failureTheranosticsJuly 13, 2026Mengdan Wang, Shuo Zhang, Tursunjan Aziz, Shiyao Zhang, Xiao Wu, Gang Li, Yan Wangovernight at 4 °C with antibodies against SMAD2/3 (CST, 8685), EP300 (CST, 57625), or H3K9ac (CST, 9649), followed by incubation with Dynabeads Protein G for 4 h. After sequential washes and elution, DNA

(4) Programmable CRISPRtune dissects the transcriptional repressive activity of MeCP2bioRxivJuly 10, 2026Jinna I. Brim, Izaiah J. Ornelas, Peter J. Colias, Nikita S. Divekar, Da Xu, Justin P. Lubin, Sophia I. Ferrel, Luis Galán Galán Palma, Mitzi G. Hernández Hernández Zamora, Rithu K. Pattali, et al.Immunoprecipitation was performed overnight at 4 °C using 2 μL of antibody (Cell Signaling Technology 9649S, 8173S). 50 μL of Protein A/G magnetic beads (Thermo Fisher 88803) per sample were washed as follows:

(5) A screen of chromatin-targeting compounds identifies TAF1 as a novel regulator of HIV latencymBioJuly 8, 2026Samuel D. Burgos, Airlie M. Ward, Manickam Ashokkumar, Kimberly P. Enders, Lindsey I. James, David M. Margolis, Edward P. BrowneAntibodies included IgG (EpiCypher, 13-0042k), H3K4me3 (EpiCypher, 13-0060k), H3K9ac (Cell Signaling, 9649S), and RNA Polymerase II (Cell Signaling, 2629). DNA was quantified with the Qubit High Sensitivity dsDNA

(6) Insulin potentiates lipopolysaccharide-induced IL-6 expression through epigenetic remodeling in adipocytes: in vitro and in vivo mechanistic studyFront ImmunolJuly 8, 2026Fatemah Bahman, Areej Al-Roub, Nadeem Akther, Ashraf Al Madhoun, Fahd Al-Mulla, Rasheed Ahmadimmunoprecipitated overnight at 4 °C using antibodies specific for histone H3 lysine 9 acetylation (H3K9ac; cat. #9649, CST), histone H3 (positive control; cat. #4620, CST), or normal rabbit IgG (negative control; cat.

(7) Chromosome condensation mechanically primes the nucleus for mitosisThe EMBO JournalJune 29, 2026Vanessa Nunes, Margarida Moura, Sara F Silva, Débora Vareiro, Nicolas Audugé, Nicolas Borghi, Jorge G Ferreiraanti-vinculin,ThermoFisher,700062 Rabbit monoclonal anti-acetyl-Histone H3 (Lys9) (C5B11),Cell Signaling Technology,9649 "Mouse monoclonal anti-Nesprin-2 (SYNE-2, F11)",Santa Cruz Biotechnology,sc-398616 Goat anti-rabbit

(8) A comprehensive pharmacological survey across heterogeneous patient-derived glioblastoma stem cell modelsiScienceJune 19, 2026Richard J.R. Elliott, Peter W.K. Nagle, Muhammad Furqan, John C. Dawson, Vanessa Smer-Barreto, Diego A. Oyarzún, Aoife McCarthy, Alison F. Munro, Camilla Drake, Gillian M. Morrison, et al.18695-1-AP Rabbit monoclonal anti-acetyl Histone H3 (Lys9) (C5B11),Cell Signaling Technology,Cat# 9649S Mouse monoclonal anti-Histone H3.1/3.2 (1D4F2),Active Motif,Cat# 61630 Rabbit monoclonal anti-phospho AKT (Ser473

(9) ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signalingiScienceJune 19, 2026Shanshan Shen, Xuetian Qian, Zhao Shi, Yuting Wu, Zitong Zhou, Shuang Nie, Yuanyuan Yu, Siqi Zhou, Kun Liu, Quan Zhou, et al.anti-H3k14ac,Cell Signaling Technology,Cat# 7627 Rabbit anti-H3k9ac,Cell Signaling Technology,Cat# 9649 Mouse anti-Sema3a,Santa Cruz Biotechnology,"Cat# sc-74555, RRID: AB_2185394" Mouse anti-Sema4d,Santa Cruz Biotechnology

(10) Nuclear export of R-loop by the DDX1 and XPO1 complex promotes senescence-associated secretory phenotype and inflammagingNat AgingJune 16, 2026Xue Hao, Bo Zhao, Qingqing Yan, Mitsutake Yano, Ayumu Matsuoka, Yuan Qi, Liping Liao, Rafal J. Zielinski, Hsin-Yao Tang, Andrew V. Kossenkov, et al.1:1,000), rabbit monoclonal anti-acetyl-histone H3 (Lys9) (C5B11) (Cell Signaling Technology, cat. no.9649; 1:500), rabbit anti-α-tubulin (Cell Signaling Technology, cat. no.2144; 1:1,000), rabbit monoclonal

(11) Changes in nuclear and actin mechanics from G1 to G2 affect nuclear integrityJournal of Cell ScienceJune 15, 2026Samantha Bunner, Katie Huang, Anish Shah, Schuyler Figueroa, Nick Lang, Catherine Chu, Nebiyat Eskndir, Mai Pho, Gianna Manning, Mindy Zheng, et al.Cell Signaling Technology), mouse anti-paxillin at 1:1000 (610052, BD Transduction Laboratories), anti-H2K9ac 1:400 (9649, Cell Signaling Technology), anti-lamin A/C at 1:10,000 (4777, Cell Signaling Technology), anti-H3K9me2-3

(12) Targeted neuronal reprogramming rescues memory and neural synchrony in Alzheimer's diseaseMol BiomedJune 10, 2026Marcos Galán-Ganga, Irene Rodríguez-Navarro, Sofía Zaballa, Alba Ramón-Lainez, Nerea Gómez-Rivada, Christian Peters, Joaquín Fernández-Irigoyen, Enrique Santamaría, M Ángeles Rabadán, Jordi Alberch, et al.1:300, #Ab5076, Abcam), anti‑H3K9me3 (rabbit, 1:300, #Ab8898, Abcam), anti‑H3K9Ac (rabbit, 1:300, #9649S, Cell Signaling), anti‑NeuN (mouse, 1:300, MAB377, Millipore/Chemicon), anti‑OLIG2 (rabbit, 1:500, AB9610,

(13) Fusobacterium nucleatum–Derived Isoleucine Exacerbates Aneurysm by Inducing Ferroptosis in Vascular Smooth Muscle CellsArterioscler Thromb Vasc BiolMay 28, 2026Liang Chen, Lu Lin, Zheyu Wang, Lu Yu, Bichen Ren, Siyuan Zhou, Penghui Wang, Yao Li, Eryi Lu, Zhihui Dongantibodies against acetylated-Lysine (1:1000, 9441S), H3 (1:1000, 4499S), acetyl-histone H3 (Lys9; 1:1000, 9649S), acetyl-histone H3 (Lys27; 1:1000, 8173S), and acetyl-histone H3 (Lys56; 1:1000, 4243S) were purchased

(14) NRSF regulation of OPRM1 through histone acetylationClinical EpigeneticsMay 19, 2026Ryan Thompson, Christina Chanantibody, anti-acetyl H3K9 antibody, or anti-acetyl H3K27 antibody Cell Signaling Technologies 9715, 9649, 8173. Control chromatin was pulled down using anti-H3 antibody. Chromatin crosslinking was reversed

(15) GV-001, An Oral Available Histone Deacetylase 6 Inhibitor for the Treatment of Autosomal Dominant Polycystic Kidney DiseaseJournal of Medicinal ChemistryMay 14, 2026Sian-Siou Wu, Pei-Yun Hung, Tsung-Yu Yeh, Po-Jui Lin, Ji-Wang Chern, Chao-Wu Yusolution) for 1 h. Membranes were incubated overnight with Acetyl-H3K9 (Cell Signaling Technology, CST#9649S), Histone H3 (Cell Signaling Technology, CST#3638S), Acetyl-Tubulin (Sigma-Aldrich, #T7451), and α-Tubulin

(16) Development of Biphenyl-Substituted Uracil-Based Hydroxamic Acids (UBHAs) as Potent HDAC Inhibitors with Pro-Apoptotic Activity in Leukemia and Prostate Cancer CellsJournal of Medicinal ChemistryMay 14, 2026Francesco Fiorentino, Giulio Bontempi, Federica Michetti, Valeria Pecci, Emanuele Fabbrizi, Daniela Passeri, Letizia Corsetti, Valentina Farini, Fabrizio Casano, Antimo Gioiello, et al.(Nouvs Biologicals NB500171, Rabbit polyclonal); Acetyl-Histone H3 Lys9 (Cell signaling technology 9649, Rabbit monoclonal); HSP90 (Santa Cruz sc-13119, mouse monoclonal). HSP90 and histone H3 were used as

(17) TIP60 acts as a wound-induced factor essential for efficient regeneration in planariaJournal of Biological ChemistryMay 11, 2026Akansha Pal, Ankit Arora, Hemant Kumar, Anna Thokchom, Bharti Jaiswal, Dasaradhi Palakodeti, Ashish Gupta(07-450), anti-trimethyl-Histone H3 (Lys27) antibody from Merck (07-449), acetyl-Histone H3 from CST (9649T), anti-Histone H3 antibody from CST (4499T), Acetyl-Histone H4 antibody from CST (2594T), anti-Histone

(18) KAT6A is essential for developmental control gene expression in neural stem and progenitor cellsPLoS GenetMay 4, 2026Anne K. Voss, Samantha Eccles, Johannes Wichmann, Waruni Abeysekera, Maria I. Bergamasco, Alexandra L. Garnham, Nishika Ranathunga, Yuqing Yang, Rory Bowden, Gordon K. Smyth, et al.sequencing. The antibodies to the following antigens were used: H3K9ac (Cell Signaling Technology, 9649), H3K14ac (Abcam, ab52946), H3K23ac (Millipore, 07355), MLL1 (Cell Signaling Technology, 14689), H3K4me3

(19) SIRT1 Downregulation by Advanced Glycation End Products Activates RANKL-Dependent Osteoclast Signaling and Drives Chondrocyte Senescence During Osteoarthritis DevelopmentAging CellMay 1, 2026Yizhou Li, Jian Wu, Rui Liu, Qiang Li, Fei Xueacetylated histone‐associated DNA fragments, an anti‐H3K27ac antibody (Cell Signaling Technology, #9649, 1:50) was used. Immunocomplexes were sequentially washed with low‐salt, high‐salt, LiCl, and TE buffers

(20) Design, Synthesis, and Biological Evaluation of Novel Triazine-Based Dual Histone Deacetylase/phosphatidylinositol 3-kinase Inhibitors for Breast Cancer TherapyChemMedChemApril 28, 2026Lara Luzietti, Gustavo Salgado Pires, Ana Ryan, Crisamie Regidor, Matthew Hiller, Danilo Sarti, Tríona Ní Chonghaile, Pedro de Sena Murteira Pinheiro, Damir Varešlija, Daniel Alencar Rodriguesanti‐acetyl α‐Tubulin (1:1000; #5335, Cell Signaling), rabbit anti‐acetyl histone H3 (Lys9) (1:1000; #9649, Cell Signaling), rabbit anti‐acetyl histone H3 (Lys14) (1:1000; #7627, Cell Signaling), and mouse anti‐GAPDH

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Oxidative medicine and cellular longevity

Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb from Cell Signaling Technology

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