Fig 2: Programmed cell death 1 (PD-1) and T-cell immunoglobulin- and mucin-domain-containing molecule-3 (TIM-3) expression on CD4+ and CD8+ T cells in bronchoalveolar lavage fluid (BALF). (A,D) Representative flow cytometric analysis of PD-1 and TIM-3 expression on CD4+ T cells (A) and CD8+ T cells (D) in BALF. (B,E) Percentages of PD-1+ cells among CD4+ T cells (B) and CD8+ T cells (E). (C,F) Percentages of TIM-3+ cells among CD4+ T cells (C) and CD8+ T cells (F). (B,C,E,F) Individual patient data as well as medians and interquartile ranges are shown. * p < 0.05; ** p < 0.01 (Mann-Whitney U test).

Fig 3: Correlation of the concentration of high mobility group box 1 (HMGB1) with the proportion of T-cell immunoglobulin- and mucin-domain-containing molecule-3 (TIM-3) positive T cells among CD4+ or CD8+ T cells in bronchoalveolar lavage fluid (BALF). (A) The concentration of HMGB1 in BALF was measured by ELISA. Individual patient data as well as medians and interquartile ranges are shown. (B,C) Correlation of the HMGB1 concentration with the proportion of TIM3+ cells among CD4+ T cells (B) or CD8+ T cells (C). Statistical analysis for Pearson’s correlation was performed using Student’s t-distribution. NS: not significant.

Fig 4: TIM-3 ligand binding is required for mitigating RICD sensitivity in early stage effector T cells.Effector T cells at day 4 (A) or day 14 (C) were treated with soluble TIM-3 (+sTIM-3) or isotype control (-sTIM-3) for 1 h prior to restimulation with 100 ng/mL of OKT3 and death assessment 24 h later. Cells were treated day 4 (B) or day 14 (D) with TIM-3 blocking antibody or an isotype antibody control for 1 h prior to restimulation with 100 ng/mL of OKT3 and death assessment 24 h later. Asterisks denote statistical significance using paired t tests: A p = 0.0399; B p = 0.0003; C p = 0.9957; D p = 0.0563.

Fig 5: A heat map presenting data on 20 immune checkpoints from a TMA containing 124 GC patient samples.The abscissa represents the patient number of 124 patients in the TMA, and the ordinate represents the expression of LSECtin, VISTA, PD-L1, IDO, TIM-3, TNFRSF14, B7M4, CEACAM1, NECTIN2, CD44, CD155, ADENOSINE, TLR4, HMGB1, SIGLEC6, FAS-L, SIGLEC15, CD73, Galectin-9 and PD-L2.