Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb 3377T from Cell Signaling Technology

Product Specs
ItemPhospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $174.00 Inquire
Catalog Number3377T
Quantity20 µl
ApplicationsW, IF-IC, FC-FP
ReactivityH, M, R, Mk, Z
TargetH3 (Ser10) phosphate
IsotypeIgG
HostRabbit
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight17

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Cell Signaling Technology

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(1) Wnt signaling patterns a central-peripheral body axis in the early-diverging metazoan Trichoplax adhaerensMol Biol EvolApril 8, 2026Guotao Di, Minjun Jin, Wanqing Li, Meng Yuan, Yifan Zhang, Chengtian Zhaoovernight at 4 °C with the primary antibody against phospho-histone H3 (pH3) (Cell Signaling Technology, #3377), diluted 1:500 in blocking solution. Afterward, secondary antibodies were applied and incubated overnight

(2) Hyperinnervation inhibits organ-level regeneration in mammalian skinCellMarch 20, 2026Hannah T. Tam, Jingyu Peng, Rebecca Freeman, Yulia Shwartz, Shlomi Brielle, Sakshi Garg, Siti Rahmayanti, Stephen J. Crocker, Devin Coon, Ya-Chieh Hsu(1:1000),Abcam,Cat#ab108986;RRID:AB_10891773 Rabbit anti Phospho-Histone H3 (Ser10) (1:2000),Cell Signaling Technology,Cat#3377S;RRID:AB_1549592 Rabbit anti RFP (pre-adsorbed) (1:1000),Rockland,Cat#600-401-379;RRID:AB_2209751 Rabbit

(3) Spatiotemporal dynamics of Mcl-1 abundance and its influence on apoptosis susceptibilitybioRxivFebruary 12, 2026Fabian Klötzer, Nadine Pollak, Anna Baatz, Batuhan Kisakol, Fiona Ginty, Daniel B. Longley, Jochen H. M. Prehn, Markus Rehmflow cytometry (FC)), mouse anti-RFP (ChromoTek 6g6, 1:1000 WB), rabbit anti-Phospho-Histone H3 (D2C2, Cell Signaling #3377 and #8552, 1:50 FC), rabbit anti-Tom20 (Cell Signaling #42406, 1:1000 WB). Secondary antibodies were

(4) Molecular basis for the activation of Aurora A and Plk1 kinases during mitotic entryThe EMBO JournalJanuary 28, 2026Anaïs Pillan, Philippine Ormancey, Celia Ben Choug, Stephen Orlicky, Nicolas Tavernier, Lucie Van Hove, Batool Ossareh-Nazari, Nicolas Joly, Frank Sicheri, Thierry Lorca, et al.Plk1,Bethyl,Cat#A300-250A Rabbit anti-GST,Cell Signaling,#2622 Rabbit anti-Phospho-Histone H3 (Ser10),Cell Signaling,Cat#3377S Rabbit anti-Histone H3,Abcam,Cat#AB1791 Mouse anti-MBP,NEB biolabs,Cat#E8032S Rabbit anti-human Greatwall,"Burgess

(5) GMCL1 controls 53BP1 stability and modulates taxane sensitivityeLifeJanuary 19, 2026Yuki Kito, Tania J González-Robles, Sharon Kaisari, Juhee Pae, Sheena Faye Garcia, Juliana Ortiz-Pacheco, Beatrix Ueberheide, Antonio Marzio, Gergely Róna, Michele Paganop53 (1:1000, Proteintech 10442–1-AP), pHistone H3 (D2C8) (Ser10, 1:1000, Cell Signaling Technology, #3377), Ubiquitin (p37) (1:1000, Cell Signaling Technology, 58395 S), USP28 (1:1,000, Proteintech 17707–1-AP),

(6) The Crosstalk Between CRL5 and APC/C E3 Ligases Regulates Metastasis and Chemosensitivity of Cancer CellsAdvanced ScienceJanuary 1, 2026Danrui Cui, Ruirui Qu, Tianqi Li, Linchen Wang, Xiaoyu Chen, Shengpeng Shao, Xue Liang, Jun Xu, Yi Sun, Xiufang Xiong, et al.(Proteintech, 20920‐1‐AP), Cyclin E1 (Cell Signaling Technology, 20808), p‐H3 (Cell Signaling Technology, 3377), and p‐Aurora A/B/C (Cell Signaling Technology, 2914), Aurora A (Cell Signaling Technology, 14 475),

(7) Targeting high-risk MYC-overexpressed osteosarcoma with an Aurora kinase inhibitor:--results from a pilot umbrella trialnpj Precision OncologyDecember 10, 2025Kai Tian, Yafei Jiang, Mengxiong Sun, Yu Lv, Jean Fan, Caixia Sun, Yujia Zhu, Yingying Yu, Xiaoyan Qiang, Peng Peng, et al.ab213480), PD-L1 (Cell Signaling Technology, 13648), Phospho-Histone H3 (Cell Signaling Technology, 3377 s), Histone H3 (Abcam, ab1791-100), Aurora A (Cell Signaling Technology, 14475S), Aurora B (Cell Signaling Technology

(8) Injury-induced Neuregulin-EGFR signaling from muscle mobilizes stem cells for whole-body regeneration in acoelsDevelopmental BiologyDecember 1, 2025Brian Stevens, Riley Popp, Heather Valera, Kyle Krueger, Christian P. Petersen10 % Horse Serum and incubation with rabbit anti-phospho-ser10-Histone3 (Cell Signaling Technology #3377S) at 1:500, then goat anti-rabbit-HRP (1:500, ThermoFisher G21234) secondary antibody, followed by tyramide

(9) Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasisNature Cell BiologyNovember 24, 2025Alberto Minetti, Omid Omrani, Christiane Brenner, Feyza Cansiz, Shinya Imada, Jonas Rösler, Saleh Khawaled, Gabriele Allies, Sven W. Meckelmann, Nadja Gebert, et al.For immunohistochemistry (IHC) experiments, rabbit monoclonal anti-pH3 (Ser10) (Cell Signaling Technologies no. 3377) antibody was used at 1:500, followed by a biotin-conjugated donkey anti-rabbit secondary antibody (1:500,

(10) The blood–brain barrier regulates brain tumor growth through the SLC36 amino acid transporter Pathetic in DrosophilaPLOS BiologyNovember 18, 2025Qian Dong, Edel Alvarez-Ochoa, Phuong-Khanh Nguyen, Paul Orih, Natasha Fahey-Lozano, Hina Kosakamoto, Fumiaki Obata, Cyrille Alexandre, Louise Y. Cheng(1:100, Abcam, #ab197788), rat anti-Dpn (1:200; Abcam, 195172), rabbit anti-pH3 (1:200, Cell Signaling, 3377), mouse anti-Elav (1:50; DSHB, 9F8A9), rabbit anti-Dcp-1-cleaved (1:100, Cell Signaling, 9578S), and

(11) N2B27 media formulations influence gastruloid developmentDevelopmentNovember 15, 2025Tina Balayo, Sharna Lunn, Pau Pascual-Mas, Ulla-Maj Fiuza, Amruta Vasudevan, Joshua D. Frenster, Joel B. Josende García, Hannah Y. Galloon, Raquel Flores Peirats, Alfonso Martinez Arias, et al.cells were stained with the primary antibodies anti-phospho-histone 3 (dilution 1:1600, Cell Signaling, 3377) and anti-cleaved-caspase 3 (dilution 1:5000, Cell Signaling, 9664). A goat-anti-rabbit Alexa-488-conjugated

(12) Benchmarking porcine pancreatic ductal organoids for drug screening applicationsEMBO Molecular MedicineNovember 4, 2025Christos Karampelias, Kaiyuan Yang, Falk J Farkas, Michael Sterr, Mireia Molina van Den Bosch, Simone Renner, Janina Fuß, Christine von Toerne, Sören Franzenburg, Tatsuya Kin, et al.Bank,F55A10 Sheep a-NEUROG3,R&D Systems,AF3444 Rabbit a-phospho (Ser10) histone 3,Cell Signaling,3377S Rabbit a-pancreatic amylase,Abcam,21156 Guinea pig a-INS,LSBio,LS-C85862-1 Guinea pig a-GCG,Takara,M182 Rabbit

(13) Squamate ventricular cardiomyocytes: Ploidy, proliferation, and heart muscle cell size in the leopard gecko ( Eublepharis macularius )Developmental DynamicsNovember 1, 2025Kathy Jacyniak, Karemna Barrera Jaimes, Minh Hanh Doan, Jordyn M. Chartrand, Matthew K. Vickaryousfor 30 min at 37°C,"Rabbit anti‐pHH3 1:500 in diluent overnight at 4°C (Cell Signaling Technology, 3377, AB_1549592)","Cy3 Goat anti‐Rabbit 1:250 in 1XPBS for 1 h at room temperature (Jackson ImmunoResearch

(14) DIAPH3 is upregulated in high-grade gliomas and linked to chromosomal instabilityNeuro-Oncology AdvancesOctober 24, 2025Caren Jabbour, Mathilde Beka, Philippe Gailly, Nicolas Tajeddine(Abcam, #AB245660; 1:2500), rabbit polyclonal anti-phospho-Histone H3 (Cell Signaling Technology, #3377; 1:1000), and rabbit monoclonal anti-GAPDH (Cell Signaling Technology, #2118; 1:10 000). After washing,

(15) Planarian microtubules form a network within muscle and regulate injury-induced genes essential for regeneration patterningDevelopmentOctober 15, 2025Xavier N. Anderson, Christian P. Petersenincubated overnight at room temperature with rabbit anti-ser10-H3P antibody (Cell Signaling Technology, 3377S) at 1:300 diluted in blocking solution. Animals were washed with PBSTx six times over 6 h prior to incubation

(16) CAF-Driven Mechanotransduction via Collagen Remodeling Accelerates Tumor Cell Cycle ProgressionGelsAugust 13, 2025Yating Xiao, Yingying Jiang, Ting Bao, Xin Hu, Xiang Wang, Xiaoning Han, Linhong Dengstained with KI67 (Abcam, Cambridge, UK, ab15580, 1:500) or Phospho-Histone H3 (CST, Danvers, MA, USA, 3377, 1:500) and secondary AF488 antibody (Thermo, Waltham, MA, USA, A-11008, 1:500). FUCCI cell-cycle phases

(17) Knockout of thyroid hormone receptor alpha a (thraa) enhances cardiac regeneration in zebrafish through metabolic and hypoxic regulationCell Commun SignalJuly 16, 2025Man Yee Cheung, Chunmei Jiang, Imtiaz Ul Hassan, Hui Wang, Donghao Guo, Daniel Wuyang Dio, Huan Yan, Jianmin Sun, Xufeng Qi, Dongqing Cai, et al.(Santa Cruz Biotechnology, sc-166524, 1:500 dilution), rabbit anti-phospho-histone H3 (Cell Signaling Technology, #3377, 1:500 dilution), at 4 °C overnight. Incubated slides were washed with 1x PBS-Tween 20 three times for

(18) Integrated bulk and single-cell RNA sequencing reveals a cuproptosis-related LncRNA prognostic signature in neuroblastomaBMC CancerJuly 1, 2025Ke Chen, Jing Wang, Shimin Yang, Jun Xiao, Luyao Wu, Zejian Li, Xiang Zhao, Xuyong Chen, Honglin Li, Jiexiong Feng, et al.Lipofectamine 3000.Functional Assays: Proliferation: Phospho-Histone H3 immunofluorescence (1:500; CST #3377). Migration: Scratch wound healing assay (10 µl pipette tip; serum-free MEM; time-lapse imaging).qPCR:

(19) PLK1-mediated PDHA1 phosphorylation drives mitochondrial dysfunction, mitophagy, and cancer progression in Cr(VI)-associated lung cancerJournal of Biological ChemistryJune 20, 2025Qiongsi Zhang, Jia Peng, Zhiguo Li, Xiongjian Rao, Derek B. Allison, Qi Qiao, Zhuangzhuang Zhang, Yifan Kong, Yanquan Zhang, Ruixin Wang, et al.(Cell Signaling Technology Cat# 3205, RRID:AB_2162926), phospho-Histone H3 (Ser10) (Cell Signaling Technology Cat# 3377, RRID:AB_1549592), GST(Cell Signaling Technology Cat# 2622, RRID:AB_331670), VDAC(Cell Signaling Technology

(20) Mongolian medicine Eerdun-Wurile promotes myocardial regeneration by regulating MVDA in zebrafishCell RegenJune 6, 2025Xianghui Chen, Xiaoting Li, Jiajun Sun, Yufeng Lin, Yuanhao Li, Xuehao Lv, Rui Zhao, Xinyue Gu, Wenxuan Wang, Yabin Xie, et al.Triton X-100) for 2 h at 22~25℃ and then incubated with a primary antibody against pH3 (1:500; Cat. No.: 3377; Cell Signalling Technology, Boston, USA) and MVD (1:500, Cat.No.:PA5-22,164; Invitrogen) overnight.

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Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb from Cell Signaling Technology

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