Fig 1: CBFB interacts with and facilitates mRNA binding of eIF4B. a FLAG IP followed by IB showing the interaction of translation initiation factors with CBFB. 10 µg/ml RNase A was added in the co-IP lysate. b Endogenous co-IP followed by IB showing interaction of CBFB, hnRNPK, and eIF4B. 10 µg/ml RNase A was added in the co-IP lysate. c In vitro translation assays showing the effect of rCBFB, rhnRNPK, and reIF4B on RUNX1 translation in a cap-dependent or -independent manner. d RIP followed by real-time PCR to show the effect of CBFB deletion on the binding of eIF4B on CBFB-bound transcripts. Error bars are SEM, n = 3 (biological); n.s., p value > 0.05 (RIP of eIF4B in WT vs. CBFB KO cells). e A model showing the role of CBFB in translation regulation through hnRNPK and eIF4B