Di-Methyl-Histone H3 (Lys9) (D85B4) XP ® Rabbit mAb 4658T from Cell Signaling Technology

Product Specs
ItemDi-Methyl-Histone H3 (Lys9) (D85B4) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $174.00 Inquire
Catalog Number4658T
Quantity20 µl
ApplicationsW, IP, IF-IC, FC-FP, ChIP, ChIP-seq, C&T
ReactivityH, M, R, Mk
TargetH3 (Lys9) dimethylate
IsotypeIgG
HostRabbit
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight17

Add to Compare List

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) The targeted cytosolic degradation of class I histone deacetylases is essential for efficient alphaherpesvirus replicationElifeJuly 9, 2026Sheng-Li Ming, Meng-Hua Du, Jia-Ming Yang, Ya-Di Guo, Jia-Jia Pan, Wei-Fei Lu, Jiang Wang, Lei Zeng, Bei-Bei Chu(2197), γ-H2AX (80312), H3 (4499), H4K8ac (2594), H4K12ac (13944), H4 (13919), H2AX (7631), H3K9ac (4658), H3K27ac (8173), UB (20326), and MDM2 (86934) were purchased from Cell Signaling Technology; H3K56ac

(2) Reduced FBXO22 skews human trophoblast fate equilibrium toward syncytialization via polyubiquitinating the CoREST complexNucleic Acids ResJune 4, 2026Hongli Li, Guangmin Song, Linwei Zhou, Man Zhang, Yun Li, Xinmi Liu, Xing Wang, Li Yang, Xinyi Tao, Richard D Cannon, et al.input control, while the remaining chromatin was incubated overnight at 4°C with H3K27ac antibody (CST, 4658) for immunoprecipitation. Then, 30 μl of Protein A/G magnetic beads (88803, ThermoFisher) were added,

(3) The Crucial Role of GPR30 and Histone Crotonylation during the Inflammatory Repair Process in Ligature-Induced PeriodontitisActa Histochem CytochemApril 25, 2026Fidya, Takumi Ishizuka, Toshiki Kubota, Kengo Kai, Yoshitaka Hishikawaanti-H4ac (Abcam, #ab177790), anti-H3K4me2 (Cell Signaling, #9725), anti-H3K9me2 (Cell Signaling, #4658), anti-H3K27me2 (Cell Signaling, #9728), anti-H3K36me2 (Cell Signaling, #2901), anti-H3K4me3 (Cell Signaling,

(4) Microsporidian Encephalitozoon hellem inhibits host mitophagy by inducing ERAD to degrade BNIP3LPLoS PathogMarch 23, 2026Ziyun Zou, Yibo Hu, Zhongxia Guan, Jiajing Chen, Qingyao Zhang, Yinze Han, Junyu Zhu, Chunxia Wang, Bing Han, Tian Li, et al.(Cell Signaling Technology, 9725; 1:2000), anti-Di-Methyl-Histone H3 (Lys9) (Cell Signaling Technology, 4658; 1:2000), Anti-SQSTM1/p62 (Abcam, Ab109012; 1:5000), Anti-TOMM20 (Abcam, ab186735, 1:2000), Anti-HMGA1(Abcam,

(5) Pharmaco-genomic characterization of pancreatic and biliary tract cancer tumoroids for drug responseiScienceMarch 20, 2026Kun Fang, Wenxin Zhang, Qing Zhao, Yan Hong, Ruiqi Wang, Biyu Yang, Yangrong Zhao, Yunkai Shi, Bing Zhou, Jian Ding, et al.were used for protein detection: anti-ERK1/2 (#4695S), anti-Phospho-ERK1/2 (#4370S) and anti-H3K9me2 (#4658S) from CST, anti-cleaved caspase3 (#R013264) and anti-cleaved PARP (#R012387) from epizyme, anti-Bim

(6) The EHMT2-MBLAC2 axis suppresses ribosomal DNA transcription in response to nucleolar DNA damageCell Death & DiseaseMarch 18, 2026Chenyue Wang, Qiutian Lu, Lianbao Cao, Simeng Zeng, Zihan Gao, Yinglong Yang, Xiaowen Liu, Shanshan Gao, Chao Dong#66689-1-Ig); EHMT2(Selleck, #F0379); MBLAC2(Santa Cruz, #SC-398153); MBLAC2(Abmart, #PS13383); H3K9me2(CST, #4658S); Lamin B1(Sharebio, #SB-AB0054); anti-Flag (Abcam, #ab20560); anti-Myc (Abclonal, #AE070); anti-β-Actin

(7) Regulation of BCL11A DNA binding and expression in human erythrocyte precursor HUDEP-2 cellsbioRxivFebruary 12, 2026Meigen Yu, Puspa Das, John R. Horton, Jujun Zhou, Jisun Lee, Tingting Hong, Yue Lu, Marcos R. Estecio, Polina A. Iakova, Abhinav K. Jain, et al.overnight at 4°C. Primary antibodies included DNMT1 (Cell Signaling Technology - CST #5032), H3K9me2 (CST #4658), H3K27me3 (CST #9733s), H3 (CST #14269), EED (CST #51673), GAPDH (CST #5174), β-globin (37–8) (Santa

(8) EpiBrain: the brain’s epigenetic landscape in a snapshotbioRxivJanuary 28, 2026Maja Johnson, Aiden F Eno, Karl Stuntzner-Gibson, James W MacDonald, Theo K Bammler, Yijie Gengmonoclonal antibodies against H3K27me3 (CST#9733), H3K27ac (CST#8173), H3K36me3 (CST#4909), H3K9me2 (CST#4658), H3K9ac (CST#9649), H3K4me1 (CST#9751), H3K4me3 (CST#5326), and 5mC (CST#28692), and mouse monoclonal

(9) G9a-mediated H3K9me2 orchestrates intestinal epithelial regeneration through epigenetic silencing of cell cycle-related genesNature CommunicationsJanuary 19, 2026Jingzhou Chen, Xiaoliang Shi, Xinyi Zhou, Ju Huang, Linghao Xia, Zhen Hu, Jiaji Gu, Xiaole Sheng, Xiaolong Ge, Xudong Fu, et al.Abcam), rabbit anti-Mono-Methyl-Histone H3K9 (#A2358, ABclonal), rabbit anti-Di-Methyl-Histone H3K9 (#4658, Cell Signaling Technology), rabbit anti-Tri-Methyl-Histone H3K9 (#A2360, ABclonal), rabbit anti- Acetyl-Histone

(10) KDM3A drives NSCLC proliferation and metastasis via H3K9 demethylation, EMT activation, and MMP-9 upregulationBiomolecules and BiomedicineNovember 3, 2025Bingqing Shi, Zhe Wang, Lei Xiu, Luyao Li, Xiaolian Yang, Guanhua Wang, Jianjun Li, Hu Wang, Yuning Han(GeneTex, 54313, 1:1000), KDM3B (Proteintech, 19915-1-AP, 1:1000), H3K9me2 (Cell Signaling Technology, 4658T, 1:2000), β-actin (Santa Cruz Biotechnology, sc-47778, 1:1000), matrix metalloproteinase-9 (MMP-9) (Santa Cruz Biotechnology

(11) The interplay between hormonal vitamin D and lipopolysaccharide signaling on human neutrophil transcriptional responsesFront ImmunolOctober 3, 2025Aiten Ismailova, Reyhaneh Salehi-Tabar, Nailya Ismailova, Olivia Dumas, James Saliba, Volker Blank, John H. White(14018-1-AP, Proteintech) ChIP antibodies were purchased from Proteintech and used at 2 µg/sample. H3K9me2 (#4658S, Cell Signaling Technology) and normal IgG rabbit antibodies (#2729S, Cell Signaling Technology) were

(12) Integrative proteogenomic characterization of Wilms tumorNat CommunAugust 19, 2025Cheng Cheng, Li Zhang, Xiaofeng Chang, Kai Chen, Tian He, Jia Shi, Fan Lv, Lijia Pan, Yangkun Wu, Qianqian Cheng, et al.CDK2 (Cat No. 2546, 1:1000), Histone H3 (Cat No. 4499, 1:1000), Di-Methyl-Histone H3 (Lys9) (Cat No. 4658, 1:1000), and EHMT2 (Cat No. 3306, 1:1000) were purchased from Cell Signaling Technology (Beverly, USA).

(13) Nrf2 Drives Epigenetic Reprogramming and Acts as the Master Regulator of KLF4 Expression and Activity in Arsenic‐Induced TransformationAdvanced ScienceAugust 4, 2025Ziwei Wang, Zhuoyue Bi, Jessica Bamrah, Yiran Qiu, Wenxuan Zhang, Bandar Saeed Almutairy, Haoyan Ji, John D. Haley, Chitra Thakur, Fei Chenantibodies against H3K4me1 (Thermofisher, cat#710795), H3K4me3 (Thermofisher, cat#711958), H3K9me3 (CST, cat#4658), H3K27Ac (CST, cat#8173), H3K27me3 (CST, cat#9733), H3K36me3 (CST, cat#4909), H4K20me3 (CST, cat#5737)

(14) Unravelling the role of epigenetic regulators during embryonic development of Rhipicephalus micropolusbioRxivJuly 11, 2025Anderson Mendonça Amarante, Daniel Martins de Oliveira, Marcos Paulo Nicolich Camargo de Souza, Manoel Fonseca-Oliveira, Antonio Galina, Serena Rosignoli, Angélica Fernandes Arcanjo, Bruno Moraes, Alessandro Paiardini, Dante Rotili, et al.Cell Signaling",8173 "Anti-H3K14ac, Cell Signaling",7627 "Anti-H3K4me3, Cell Signaling",9727 "Anti-H3K9me2, Cell Signaling",4658 "Anti-H3K9me3, Cell Signaling",13969 "Anti-H3K27me3, Cell Signaling",9733 "Anti-m6A, Invitrogen",MA5–35350 "Anti-m5C,

(15) BRD4 acts as a transcriptional repressor of RhoB to inhibit terminal erythropoiesisJournal of Hematology & OncologyJuly 1, 2025Yijin Chen, Dawei Huo, Ye Meng, Jie Zhang, Mengmeng Huang, Qian Luo, Yulin Xu, Haiqiong Zheng, Yingli Han, Xiangjun Zeng, et al.A301-985 A), CDK9 (Bethyl, A303-493 A), Pol2-S5P (CST, 13523s), Pol2-S2P (CST, 13499s), and H3K9me2 (CST, 4658).The eluted DNA fragments were purified with a DNA purification kit (Qiagen, 28106). Primers for ChIP-qPCR

(16) Synaptic Deficits in Adnp‐Mutant Mice Are Ameliorated by Histone Demethylase LSD1 InhibitionAutism ResearchJuly 1, 2025Chih‐Hung Lin, Yong Ren, Kin Wai Tam, Megan Conrow‐Graham, Zhen Yan(Cell Signaling, 9649S, 1:1000), anti-H3K4me2 (Cell Signaling, 9725S, 1:1000), anti-H3K9me2 (Cell Signaling, 4658S, 1:1000), anti-H3K9me3 (Invitrogen, PA5-31910, 1:1000), anti-Adnp (C-terminus, Invitrogen, PA5-52286,

(17) Histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticlesSci RepJuly 1, 2025Xiaoxu Zhao, Liping Zhao, Hui Lin, Xiumei WangAc-H3K9 (cat. no. 9649), Ac-H3K14 (cat. no. 7627), Tri-Me-H3K4 (cat. no. 9751), Di-Me-H3K9 (cat. no. 4658), and Tri-Me-H3K27 (cat. no. 9733) (Cell Signaling Technology, Inc., MA, USA) (1:1000), and Ac-H3 (global)

(18) Regulation of epigenetics and chromosome structure by human ORC2Cell ReportsJune 24, 2025Zhangli Su, Mengxue Tian, Etsuko Shibata, Yoshiyuki Shibata, Tianyi Yang, Zhenjia Wang, Fulai Jin, Chongzhi Zang, Anindya DuttaRRID: AB_2070987 Di-Methyl-Histone H3 (Lys9) (D85B4) XP Rabbit mAb,Cell Signaling Technology,Cat #4658; RRID: AB_10544405 HBO1/MYST2 (D4N3F) Rabbit mAb,Cell Signaling Technology,Cat #58418; RRID: AB_2799547 Histone H3 (D1H2) XP Rabbit mAb

(19) Constitutive heterochromatin controls nuclear mechanics, morphology, and integrity through H3K9me3 mediated chromocenter compactionNucleusApril 9, 2025Gianna Manning, Andy Li, Nebiyat Eskndir, Marilena Currey, Andrew D. StephensLamin A/C 1:1,000 (4777, Cell Signaling Technologies), H3K9me3 1:1,000 (8898, Abcam), H3K9me2 1:500 (4658, Cell Signaling Technologies), H3K27me3 1:600 (9733, Cell Signaling Technologies), Lamin A/C 1:200 (4C11,

(20) Quantitation of global histone post-translational modifications reveal anti-inflammatory epigenetic mechanisms of liquiritigenin based on the optimized super-SILAC strategyFrontiers in Cell and Developmental BiologyMarch 27, 2025Ping Liu, Jun Zhang, Jingdan Zhang, Yucheng Yuan, Zhiqing Liu, Sixian Chen, Kaifeng Chen, Li Dong, Zhiyuan Cheng, Yinan Zhang, et al.trimethyl-histone H3 (Lys4) antibody (Cell Signaling, 9727S), dimethyl-histone H3 (Lys9) (D85B4) antibody (Cell Signaling, 4658S), trimethyl-histone H3 (Lys9) (D4W1U) antibody (Cell Signaling, 13969T), dimethyl-histone H3 (Lys27)

Be the First to Write a Review!

Di-Methyl-Histone H3 (Lys9) (D85B4) XP ® Rabbit mAb from Cell Signaling Technology

Description

Cell Signaling Technology

Citations (130)

Reviews

Di-Methyl-Histone H3 (Lys9) (D85B4) XP ® Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map