Acetyl-Histone H3 (Lys27) (D5E4) XP ® Rabbit mAb 8173T from Cell Signaling Technology

Product Specs
ItemAcetyl-Histone H3 (Lys27) (D5E4) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $180.00 Inquire
Catalog Number8173T
Quantity20 µl
ApplicationsW, W-S, IF-IC, FC-FP, ChIP, ChIP-seq, C&R, C&T
ReactivityH, M, R, Mk
TargetH3 (Lys27) acetylate
IsotypeIgG
HostRabbit
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight17

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) E3 ligase UHRF2 hijacks nuclear TBK1 to epigenetically repress type I interferons expressionInt J Biol SciMay 18, 2026Wenwen Huang, Renjie Song, Qicong Shen, Yang Shi, Xi Wang, Nan Li, Qian Zhang, Xuetao CaoAnti-L-Lactyl-Histone H4 (Lys12) (1:1000; PTMab, PTM-1411RM), Anti-H3K27ac (1:1000; Cell Signaling Technology, 8173), Anti- Histone H3 (1:1000; PTMab, PTM-1001RM). The secondary antibodies were antimouse IgG-HRP (1:2,000;

(2) mTOR inhibition promotes ATRA-induced cancer cell differentiation by overcoming a metabolic hyperactive stateJ Transl MedMay 11, 2026Liuqingqing Zhang, Guiqiyang Xiang, Ping Zhu, Shuting Yu, Fangying Jiang, Shuai Wang, Jinzeng Wang, Yao Dai, Shengyue Wang, Yun Tan, et al.manufacturer’s protocol [35]. The primary antibody was the anti-H3K27ac antibody (Cell Signaling Technology, #8173). The secondary antibody used the anti-rabbit antibody with high affinity for protein A/G and without

(3) Cis-regulatory evolution shapes facial diversity in birds and mammalsSci AdvMay 8, 2026Stella Kyomen, Louk W. G. Seton, Laura E. Cook, Elio Escamilla-Vega, Andrea P. Murillo-Rincón, Alexander Jacobsen, Amor Damatac, Carsten Fortmann-Grote, Janina Fuss, Axel Visel, et al.in size. Chromatin was incubated for 3 hours at 4°C with 10 μg of Cell Signaling H3K27ac antibody (8173S; lot: 106590037-6). Protein A and G Dynabeads (Invitrogen) were then added to this chromatin/antibody

(4) Rewiring Oncogenic Transcriptional Complexes with Domain-ALTeration Chimeras (DALTACs) in Prostate CancerbioRxivMay 5, 2026Jie Luo, Jianzhang Yang, Jean Ching-Yi Tien, Mi Wang, Sumit Das, Weiguo Xiang, Eleanor Young, Jelena Tosovic, Rahul Mannan, Jocelyn Cai, et al.(Cell Signaling Technology: 34156S); H2BK16ac (Abcam: ab177427); H3K27ac (Cell Signaling Technology: 8173S); H2B (Active Motif: 39210); H3 (Cell Signaling Technology: 3638S); c-Myc (Cell Signaling Technology:

(5) Polycomb shapes active chromatin and promoter bivalency during ovarian reserve formation and activationbioRxivApril 30, 2026Mengwen Hu, Yasuhisa Munakata, Yu-Han Yeh, Neil Hunter, Richard M. Schultz, Satoshi H. Namekawaanti-H3K4me3 (1/50; Active Motif; 39159) and rabbit anti-H3K27ac (1/50; Cell Signaling Technology; 8173). CUT&Tag libraries were sequenced on the HiSeq 4000 system (Illumina) with 150-bp paired-end reads. CUT&Tag

(6) The Crucial Role of GPR30 and Histone Crotonylation during the Inflammatory Repair Process in Ligature-Induced PeriodontitisActa Histochem CytochemApril 25, 2026Fidya, Takumi Ishizuka, Toshiki Kubota, Kengo Kai, Yoshitaka HishikawaPAI-310B), anti-GPR30 (Abcam, #ab39742), anti-H3K9ac (Cell Signaling, #9643), anti-H3K27ac (Cell Signaling, #8173), anti-H4ac (Abcam, #ab177790), anti-H3K4me2 (Cell Signaling, #9725), anti-H3K9me2 (Cell Signaling,

(7) Quantitative dissection of the metastatic cascade at single colony resolutionbioRxivApril 22, 2026Chris Roberts, Andy Xu, Xiangwei Fang, Adrienne Visani, Chien-Wei Peng, Xulei Qin, Irenaeus C. C. Chan, Madeline Dunterman, David A. Giles, Yaoxuan You, et al.H3 (Cell Signaling, 4499S, 1:1000), CDX2 (Invitrogen, MA5–35215, 1:2000), H3K27Ac (Cell Signaling, 8173S, 1:1000), Goat-anti-Rabbit IgG Antibody, HRP-conjugate (Sigma-Aldrich, 12–348), Goat-anti-Mouse IgG Antibody, HRP-conjugate

(8) Lysine Acetyltransferase 6A Drives M1 Macrophage Polarization Through Metabolic Reprogramming in Sepsis-Induced Acute Lung InjuryBiomoleculesApril 20, 2026Xin Wang, Junlin Chen, Yimei Lai, Yumeng Wang, Kaixia Hu, Mengshi Wu, Niansheng Yang, Yuefang HuangH3K9ac (1:1000, Cell Signaling Technology, Cat# 9649T), H3K27ac (1:1000, Cell Signaling Technology, Cat# 8173T), Histone H3 (1:2000, Cell Signaling Technology, Cat# 4499T) and β-actin (1:1000, Servicebio, Wuhan,

(9) HDAC Inhibition Induces Transient Phenotypic Inertia in Dormant OCCC Spheroids by Derepression of Cell Cycle GenesCellsApril 10, 2026Sylvia Cheng, Bart Kolendowski, Yudith Ramos-Valdes, Trevor G. Shepherd, Gabriel E. DiMattiaCaspase 3 (cleaved),"Cell Signaling Technology (Danvers, MA, USA)",9661 H3K27Ac,Cell Signaling Technology,8173 H3K27me3,Cell Signaling Technology,9733 H3K4me1,Cell Signaling Technology,5326 H3K4me2,Cell Signaling Technology

(10) RB loss modulates chromatin organization by regulating cohesin-dependent loops and enhancer-promoter interactionsNat CommunApril 8, 2026Hanjun Lee, Ioanna-Maria Gkotinakou, Connor G. McGrath, Badri Krishnan, Sambhavi Animesh, Isabella Salinas, Robert Morris, Moshe Sade-Feldman, Wilhelm Haas, Michael S. Lawrence, et al.from 1000 ng of lysates were incubated with 300 ng of antibody against H3K27ac (Cell Signaling, Cat. # 8173) overnight for chromatin immunoprecipitation. The antibody-protein-DNA complex was pulled down with

(11) YAP1 converts circELP2-mediated biochemical signals to mechanical forces through promoting cytoskeleton remodeling in pulmonary fibrosisInternational Journal of Biological SciencesApril 8, 2026Ruiqiong Li, Songzi Zhang, Jinjin Zhang, Haitong Zhang, Yujie Wang, Changjun Lv, Bo Liu, Lihua Ren, Xiaodong Song, Fang Wangcollected as the 2% Input. Add the immunoprecipitation antibody of H3K27ac (Cell Signaling Technology, USA, 8173S) or YAP1 (Proteintech, China, 13584-1-AP), Normal Rabbit IgG (Cell Signaling Technology, USA, #2729)

(12) Intermittent Fasting Enhances Genome Integrity and Cytoprotective Pathways via (BHB) β-Hydroxybutyrate Signaling and Chromatin RemodelingFASEB JMarch 31, 2026Hadar Parnas, Joanna Bartman, Tali Rosenberg, Ronit Yosofov, Noa Gabsi, Asaf MarcoPTM Biolab, Chicago, IL, cat# PTM‐1250, RRID: AB_3076399), H3K27ac (Cell Signaling, Danvers, MA, cat# 8173, RRID: AB_10949503), rabbit anti‐Hif1a (1:1000; Invitrogen, Carlsbad, CA, cat# PA1‐16601, RRID: AB_2117128),

(13) FOXA1 loss drives basal/squamous de-differentiation of prostate cancer and induces an immunosuppressive tumor microenvironmentNature CommunicationsMarch 28, 2026Lourdes Brea, Hongshun Shi, Viriya Keo, Jing Huang, Liu Peng, Qi Chu, Wanqing Xie, Yinghua Xie, Sambhavi Senthil, Matthew T. Breneman, et al.the following antibodies overnight at 4 °C with rotation: FOXA1 ab23738 (5 µg; Abcam) and H3K27ac CST 8173S (1:100). 1 µg drosophila H3 antibody (Active motif Cat# 61686) was also added per sample. Then, protein

(14) Hat1 Orchestrates Heterochromatin Inheritance by Regulating Localization of H3K9 MethyltransferasesbioRxivMarch 25, 2026Caden J. Martin, Liudmila V. Popova, Prabakaran Nagarajan, Elizabeth A. Oser, Callie M. Lovejoy, Benjamin D. Sunkel, Benjamin Z. Stanton, Michael A. Freitas, Mark R. Parthun,Cell Signaling,5326,CUT&Tag H3K9ac,Cell Signaling,9649,CUT&Tag H3K14ac,Abcam,ab52946,CUT&Tag H3K27ac,Cell Signaling,8173,CUT&Tag H4K5ac,Abcam,ab51997,CUT&Tag H4K12ac,Abcam,ab46983,CUT&Tag Lamin B1,Abcam,ab16048,CUT&RUN Lamin

(15) CDK12/CDK13 inhibition disrupts transcriptional elongation and replication fork progression in glioblastomaEMBO Molecular MedicineMarch 25, 2026Silje Lier, Sara B Markusson, Anja Kocijancic, Martine Narum, Solveig O Lund, Bianka Böllering, Anuja Lipsa, Mirra L C Søegaard, Idun D Rein, Petra Santha, et al.,MBL international,"MABI0603, RRID: AB_2728736" Rabbit monoclonal anti-Acetyl-Histone H3 (Lys27) (D5E4),CST,#8173 Rabbit monoclonal anti-tri-methyl-histone H3 (K27) (C36B11),CST,9733S Rabbit monoclonal anti-gamma H2A.X (phospho S139

(16) Genome-wide CRISPR screen identifies a cytokine-enhancer circuit driving HIF-2α activation in renal cancerJ Clin InvestMarch 24, 2026Jun Fang, Jeremy M. Simon, Tao Wang, Yunpeng Gao, Xianju Bi, Lianxin Hu, Chengheng Liao, Cheng Zhang, Yayoi Adachi, Jin Zhou, et al.extracted with RIPA lysis buffer. Chromatin fragments were incubated with anti-H3K27ac antibody (CST, 8173) overnight at 4°C for ChIP. The antibody-protein-DNA complex was pulled down with protein A/G–coated

(17) Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunityEMBO Molecular MedicineMarch 23, 2026Xingyu Chen, Yang Zhao, Yunfei Xie, Tianyi Liu, Haocheng Wang, Xiao Wang, Xuefei Guo, Fuping You(catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L

(18) Microsporidian Encephalitozoon hellem inhibits host mitophagy by inducing ERAD to degrade BNIP3LPLoS PathogMarch 23, 2026Ziyun Zou, Yibo Hu, Zhongxia Guan, Jiajing Chen, Qingyao Zhang, Yinze Han, Junyu Zhu, Chunxia Wang, Bing Han, Tian Li, et al.(Cell Signaling Technology, 7627; 1:2000), anti-H3K18ac (Beyotime, AG3829; 1:2000), anti-H3K27ac (Cell Signaling Technology, 8173; 1:2000), anti-Di-Methyl-Histone H3 (Lys4) (Cell Signaling Technology, 9725; 1:2000), anti-Di-Methyl-Histone H3 (Lys9

(19) β-hydroxybutyrate enhances the metabolic fitness of CAR T cells in cancerCellMarch 19, 2026Shan Liu, Puneeth Guruprasad, Ranjani Ramasubramanian, Bhoomi Madhu, Luca Paruzzo, Kecheng Han, Andre Kelly, Alexander Shestov, He N. Xu, Alberto Carturan, et al.#E7335S). The antibody against Acetyl-Histone H3 (Lys27) was obtained from Cell Signaling Technology (#8173T). Library quality and size distribution were assessed with the Bioanalyzer High Sensitivity DNA Analysis

(20) The MEK1/2-IRF4 axis fosters T follicular helper cell differentiationand antitumor humoral immune responseJournal of Translational MedicineMarch 16, 2026Shuan Ran, Song Wang, Ran Li, Longyong Lai, Jizhang Yu, Xi Zhang, Yuan Li, Weicong Ye, Junjie Zong, Xiaohan Li, et al.stained with Acetyl-Histone H3 (Lys27) (D5E4) Rabbit Monoclonal Antibody(Cell Signaling Technology, #8173), Acetyl-Histone H3 (Lys9) (C5B11) Rabbit Monoclonal Antibody (Cell Signaling Technology, #9649), Tri-Methyl-Histone

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Acetyl-Histone H3 (Lys27) (D5E4) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Acetyl-Histone H3 (Lys27) (D5E4) XP ® Rabbit mAb from Cell Signaling Technology

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