Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb 9751T from Cell Signaling Technology

Product Specs
ItemTri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb
CompanyCell Signaling Technology
Price $163.00 Inquire
Catalog Number9751T
Quantity20 µl
ApplicationsW, IHC-P, IF-IC, FC-FP, ChIP, ChIP-seq, C&R, C&T
ReactivityH, M, R, Mk, Dm, Sc
TargetH3 (Lys4) trimethylate
IsotypeIgG
HostRabbit
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight17

Questions and Answers

Can this antibody be used to study the role of histone methylation in cancer?

Yes, the Tri-Methyl-Histone H3 (Lys4) antibody has been utilized in various studies to investigate histone modifications associated with cancer. For instance, it was used in a study examining the role of histone methylation in the context of DNA damage response and transcriptional regulation in cancer cells, highlighting its relevance in understanding epigenetic alterations in malignancies ().

Is this antibody effective for chromatin immunoprecipitation experiments?

The Tri-Methyl-Histone H3 (Lys4) antibody has been shown to work effectively in chromatin immunoprecipitation (ChIP) assays, as evidenced by its use in studies that analyze histone modifications in relation to gene expression and chromatin dynamics (; ).

What species can this antibody be used with?

This antibody is reactive with multiple species, including human, mouse, rat, non-human primates, yeast/fungi, and Drosophila/arthropods, making it versatile for various biological studies across different model organisms.

How has this antibody been applied in recent research?

Recent studies have employed the Tri-Methyl-Histone H3 (Lys4) antibody to explore mechanisms of gene regulation and chromatin remodeling in response to various stimuli, such as TGF-β signaling in epithelial-mesenchymal transition and the effects of oxidative stress on chromatin structure (; ).

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Autism-like phenotypes and increased NMDAR2D expression in mice with KDM5B histone lysine demethylase deficiencySci AdvMay 22, 2026Leticia Pérez-Sisqués, Shail U. Bhatt, Angela Caruso, Josephine L. Robb, Alex P. A. Donovan, Rosemary Bamford, Alejo Torres-Cano, Shoshana Spring, Eleanor Hendy, Talia E. Gileadi, et al.dilution unless stated otherwise): anti-KDM5B (Abcam, #181089), anti-H3K4me3 (Cell Signaling Technology, #9751), anti–total H3 (Cell Signaling Technology, #9715), HSP90 (Santa Cruz Biotechnology, sc13119), actin

(2) A RIPK2 activity signature in prostate cancer: Modulation by RIPK2 inhibition and clinical associationTransl OncolMay 17, 2026Ahmed M. Elgehama, Qian Yang, Jaceline Gislaine Pires Sanches, Zaoke He, Lauryn Ruegg, Sungyong You, Wei YangpSTAT3 (CST, #9145T), STAT3 (CST, $12640T), c-Myc (Abcam, ab32072), KDM5A (CST, #3876T), H3K4me3 (CST, #9751T), H3 (CST, #9715S), and GAPDH (CST, 51332S). After washing, membranes were incubated for 1 h at RT with

(3) Whole-genome doubling drives immune evasion by silencing antigen presentationCancer CellMay 7, 2026Pierre Foidart, Zheqi Li, Xinran Cai, Marco Seehawer, Daniel D. Brown, Amatullah Tawawalla, Pilar Baldominos, Salma Parvin, Jun Nishida, Ernesto Rojas-Jimenez, et al.antibody,Abcam,"Cat#ab9053, RRID:AB_306969" Tri-Methyl-Histone H3 (Lys4) (C42D8),Cell Signaling Technology,"CAT#9751S, RRID:AB_2616028" Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729),Abcam,"CAT#4729, RRID:AB_2118291" Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb

(4) Hyperglycemia- induced innate immune tolerance involves the metabolic and epigenetic rewiring in human alveolar macrophagesFront ImmunolMay 7, 2026Jiang Wang, Na Yuan, Bin Wang, YongHui Qiu, Bo Wang, LiXin Xieincubate overnight at 4 °C with primary antibodies, specifically anti-H3K4me3 (rabbit monoclonal, Cat# 9751, CST) and control IgG (rabbit monoclonal, Cat# 3900S, CST). Subsequently, the unbound primary antibodies

(5) Adam13 interacts with large protein complexes to regulate histone modification and gene expressionFront Cell Dev BiolMay 7, 2026Ankit Pandey, Helene Cousin, Shiv Kumar, Louis Taylor, Ashmita Chander, Kelsey Coppenrath, Nikko-Ideen Shaidani, Marko Horb, Dominique AlfandariPCRP-ARID3A-1E9, RRID: AB_2618410); rabbit monoclonal to H3K4me3 (Cell Signaling Technology; cat. no. 9751, RRID: AB_2616028); rabbit monoclonal to KMT2α (Cell Signaling Technology; cat. no. 14197, RRID: AB_2688010);

(6) CoCUT&Tag maps linked chromatin states at single-molecule, single-cell resolutionbioRxivMay 5, 2026Wenjie Sun, Emma Baird, Harry J. Ashbaugh, Alexandria P. Eiken, Derek H. Janssensperformed the best in CoCUT&Tag assays: rabbit anti-H3K4me3 (Cell Signaling Technologies; cat. no. 9751), mouse anti-H3K4me3 (Diagenode; cat no. C15200152), rabbit anti-H3K27me3 (Cell Signaling Technologies;

(7) Folate-dependent one-carbon metabolism controls meiotic and post-meiotic epigenome remodeling in the male germlinebioRxivMay 5, 2026Ai Ikuyo, Nozomu Fuse, Masaru Mori, Akiyoshi Hirayama, Yuto Yamada, Tatsuya Nakamura, Tomoko Sagi, Kai Otsuka, Satoshi H. Namekawa, Tomoyoshi Soga, et al.included: anti-H1t (kindly provided by Dr. Mary Ann Handel), anti-γH2AX (Millipore), anti-H3K4me3 (#9751; Cell Signaling Technology), anti-H3 (MABI), anti-H3K27me3 (Diagenode; C15410195), and anti-H3K9me2

(8) Embryonic stem cell factors DPPA2/4 amplify active H3K4me3–H2AK119ub chromatin domains in non-small cell lung cancerGenes & DevelopmentMay 4, 2026Janith A. Seneviratne, Clare L. Crisp, Eleanor Glancy, Natalie Choy, Winnie Tan, Matthew Neve, Melanie Stammers, Tongtong Wang, Ruby Johnstone, Arshnoor Kaur, et al.PA5-40421), 1 µg of SUZ12 (Cell Signaling Technology 3737), 0.5 µg of H3K4me3 (Cell Signaling Technology 9751), 0.5 µg of H3K27me3 (Cell Signaling Technology 9733), 0.5 µg of H2AK119ub (Cell Signaling Technology

(9) Nanoparticle‐Mediated Immunometabolic‐Epigenetic Remodeling Enhances Schwann Cell‐Macrophage Interaction for Sciatic Nerve RegenerationAdvanced ScienceMay 1, 2026Wenying Xu, Rongrong Wu, Zhicheng Liu, Hongyu Yan, Huan Zhang, Yuanyi Zheng, Xiaojun Caiincubated overnight at 4°C with primary antibodies diluted 1:1000. The antibodies targeted H3K4me3 (CST, #9751), H3K9me3 (Abcam, #ab176916), H3K27me3 (CST, #9733), Histone H3 (Invitrogen, #PA516183), and β‐actin

(10) Targeting DNA Polymerase Epsilon Induces Tumor Clearance and Activates an NF-κB-Mediated Inflammatory Response in Triple Negative Breast CancerCancer ResApril 21, 2026Elizabeth F. Sher, Kenji M. Fujihara, Anthony Tao, Paul Sastourne-Haletou, Diana Erenburg, Vladislav O. Sviderskiy, Hannan Mir, Triantafyllia Karakousi, Cynthia A. Loomis, Jiehui Deng, et al.isotype control antibody (1:20 dilution, CST #66362) and 2μL of H3K4me3 antibody (1:50 dilution, CST #9751) per reaction. DNA was purified by phenol/chloroform extraction, and all DNA was utilized for library

(11) Locus-specific proteomics identifies novel regulators of Epstein-Barr virus lytic reactivationJournal of VirologyApril 21, 2026Daniel E. Greaves, Ildar Gabaev, James C. Williamson, Stuart Bloor, Sergio Martinez Cuesta, Nicholas J. Matheson, Lori Frappier, Jérôme Déjardin, Paul J. Lehner(Cell Signaling #9733, used for immunoblot, ChIP-PCR, and FACS), rabbit α-H3K4me3 (Cell Signaling #9751, used for immunoblot, ChIP, and FACS), rabbit α-H3K9me3 (Abcam ab8898, used for immunoblot and FACS),

(12) Single-cell Analysis Reveals Essential lncRNAs Regulating Human Trophoblast Lineage DifferentiationLife MedicineApril 17, 2026Weiyi Zhang, Chunyan Le, Shanru Yi, Wenqiang Liu, Xiaoyu Liu, Zhen Yin, Hong Wang, Rui Gao, Shaorong Gao, Jiayu Chenprimary antibodies were utilized: anti-IgG (Vazyme, Ab207-01), anti-H3K4me3 (Cell Signaling Technology, 9751S), anti-H3K27me3 (Diagenode, C15410195), and anti-H3K9ac (Abcam, ab4441). All primer sequences employed

(13) PHF2 regulates grip strength via demethylation at the promoter region of the Mef2ciScienceApril 17, 2026Taku Fukushima, Taiju Fujioka, Yuuki Imai, Satoru Masubuchi, Iori Sakakibaraprimary antibodies: anti-PHF2 (3497, CST), anti-H3K9me2 (ab1220, Abcam, Cambridge, UK), anti-H3K4me3 (9751, CST), or normal rabbit IgG as a negative control. After washing, samples were incubated with 1.5 μL

(14) Nicotinamide N-methyltransferase as a therapeutic target in taxane-resistant castration-resistant prostate cancerCell Death DiscoveryApril 17, 2026Buse Cevatemre, Ezgi Karyemez, Ipek Bulut, Hamzah Syed, Mehmet Gönen, Ahmet Tarik Baykal, Tugba Bagci-Onder, Ceyda Acilan(Lys27) (Cell Signaling Technologies, 9733); Tri-Methyl-Histone H3 (Lys4) (Cell Signaling Technologies, 9751); Histone H3 (Abcam, ab18521). SDS-PAGE and Western blotting (WB) Proteins were isolated and separated,

(15) Inhibiting LSD1 unlocks retinoid AP-1 programming to activate epithelial immunity and skin tumor suppressionJournal of Clinical InvestigationApril 15, 2026Nina Kuprasertkul, Alyssa F. Moore, Carina A. D'souza, Julia Chini, Eun-Kyung Ko, Sijia Huang, Shuo Zhang, Ashley S. Anderson, Shaun Egolf, Laura V. Pinheiro, et al.mice were used. Antibodies included H3K4me2 (Abcam ab7766, 2 μL), H3K4me3 (Cell Signaling Technology 9751T, 2 μL), and IgG (Cell Signaling Technology 66362S, 5 μL) (Supplemental Table 12). DNA was extracted

(16) Antigenic cancer persister cells survive direct T cell attackbioRxivApril 12, 2026Michael X. Wang, Brandon E. Mauch, August F. Williams, Tania Barazande-Pour, Filipe Araujo Araujo Hoffmann, Sophie H. Harris, Cooper P. Lathrop, Claire E. Turkal, Bryan S. Yung, Michelle H. Paw, et al.NRF2 (CST #12721, 1:1000), SLC7A11 (CST #12691, 1:1000), H3K14ac (CST #7627, 1:1000), H3K4me3 (CST #9751, 1:1000), H3K9me3 (CST #13969, 1:1000), H3K27me3 (CST #9733T, 1:1000), Histone H3 (CST #3638, 1:1000),

(17) HDAC Inhibition Induces Transient Phenotypic Inertia in Dormant OCCC Spheroids by Derepression of Cell Cycle GenesCellsApril 10, 2026Sylvia Cheng, Bart Kolendowski, Yudith Ramos-Valdes, Trevor G. Shepherd, Gabriel E. DiMattia,Cell Signaling Technology,5326 H3K4me2,Cell Signaling Technology,9725 H3K4me3,Cell Signaling Technology,9751 Histone H3,Cell Signaling Technology,4499 Ki67,"Abcam (Cambridge, UK)",ab16667 p-Histone H3 (Ser28),Cell Signaling Technology

(18) Apoptotic cell clearance triggers epithelial fate reprogramming during prostate regressionCell Death & DiseaseApril 10, 2026Adda-Lee Graham-Paquin, Deepak Saini, Sophie Viala, Mara KM Whitford, Mathieu Tremblay, William A. Pastor, Maxime Bouchard, Luke McCaffreyChIP Histone-H3,Rabbit,Abcam #ab1791,1:10 000 Tacstd2,Goat,R&D Systems BAF1122,1:100 IF H3K4me3,Rabbit,Cell signalling #9751S,1:50 ChIP CD45,Rabbit,Abcam #ab10558,1:200 IF Antibodies used in study.

(19) PAF1C restores transcription after DNA damage independently of promoting histone mark depositionEMBO ReportsApril 8, 2026Janne J M van Schie, Bram A F J de Groot, Diana van den Heuvel, Martijn S Luijsterburg(DM1A)",aML#008 Rabbit anti-HSPA4,"Novus Biologicals, NBP1-81696",aML#114 Rabbit anti-H3K4me3,"Cell Signaling, #9751S",aML#237 Rabbit anti-DOT1L,"Cell Signaling, #77087 (D1W4Z)",aML#216 Rabbit anti-H3K79me2,"Abcam, ab3594",aML#227 Rabbit

(20) The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathwayTranslational OncologyApril 1, 2026Chunli Wang, Dandan Shen, Nanshan Lin, Panjie Wang, Yaping Bi, Huina Hu, Yujia Shang, Chenchen Ren, Li Yang, Qisheng MaBoston, MA, USA), H3K4me1(1:2000, No.5326T, CST), H3K4me2(1:2000, No.9725S, CST), H3K4me3(1:2000, No. 9751T, CST), p62/SQSTM1(1:2000, No.5114T, CST), LC3B(1:2000, Cat# AF4650, Affinity Biosciences), Beclin-1(1:2000,

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Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb from Cell Signaling Technology

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Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb from Cell Signaling Technology

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