Fig 2: Purkinje cells and unipolar brush cells are not affected following Bdnf loss. Overviews of parasagittal cerebellar sections from 6-month-old control (A) and Bdnf CKO (B) mice labeled with Calb1 (magenta) and Calb2 (green). Higher magnifications of control (A1,2) and Bdnf CKO (B1,2) mice show no difference in unipolar brush cells (UBC) and Purkinje cells between controls and mutants (p > 0.05, n = 4, 4, respectively). Bars indicate 100 μm.

Fig 3: Rbm24 deficiency impedes adult neurogenesis in the OB. (A) Representative images of BrdU/NeuN co-staining in the OB from 3-month-old CTL and UKO mice. Scale bar, 100 μm. (B) Quantification of BrdU+ and BrdU+/NeuN+ cells in the OB from 3-month-old CTL and UKO mice (n = 4 mice for each group). (C-D) Representative images and quantification of TUNEL+ cells in the OB from 3-month-old CTL and UKO mice (n = 4 mice for each group). Scale bar, 100 μm. (E-I) Representative images and quantification of TH+ (E, G), CalB+ (F, G), CalR+ (H, I) cells in the OB from 4-month-old CTL and UKO mice 60 days after TAM administration (n = 4 mice for each group). Scale bar, 100 μm. (J) Representative traces of sIPSC of M/Ts in the OB from 4-month-old CTL and UKO mice. Scale bar, 40 pA and 20 s. (K) Cumulative curves of sIPSC interevent interval of M/Ts in the OB from 4-month-old CTL and UKO mice. (L) Quantification of sIPSC frequency of M/Ts in (J) (n = 4 mice for each group). (M) Cumulative curves of sIPSC amplitude of M/Ts in the OB from 4-month-old CTL and UKO mice. (N) Quantification of sIPSC amplitude of M/Ts in (J) (n = 4 mice for each group). Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; n.s., not significant. OB: olfactory bulb; GL: glomerular layer; GCL: granule cell layer.