Fig 1: A-miR-429 increases the surface expression of GluA1 in APP23/PS45 mice. (A)–(J) Protein levels of total GluA1 (B), GluA2 (C), GluA3 (D), GluA4 (E), synaptic GluA1 (G) and synaptic GluA2 (H), synaptic GluA3 (I), synaptic GluA4 (J) in APP23/PS45 mice treated with A-miR-429 or A-scramble (n = 4–7). (K)–(N) Protein levels of total pGluA1 S831 (L), the ratio of pGluA1 S831 and total GluA1 (M) and total pGluA1 S845 (N) in APP23/PS45 mice treated with A-miR-429 or A-scramble (n = 5–7). (O)–(P) Immunofluorescent staining for GluA1 and pGluA1 S831 in APP23/PS45 mice treated with A-miR-429 or A-scramble. Scale bar, 100 μm. (Q) The relative levels of total GluA1, pGluA1 S831 and the ratio of pGluA1 S831 and total GluA1 in APP23/PS45 mice of different ages. (R)–(S) Protein levels of surface HA-tagged GluA1 in N2A cells transfected with 1–16 μg of GluA1-HA or GluA1-S831D-HA plasmid (n = 7). Data are expressed as mean ± SEM. ns, not significant; ∗P < 0.05, ∗∗P < 0.01.