Mono-Methyl-Histone H3 (Lys4) (D1A9) XP ® Rabbit mAb 5326T from Cell Signaling Technology

Product Specs
ItemMono-Methyl-Histone H3 (Lys4) (D1A9) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $174.00 Inquire
Catalog Number5326T
Quantity20 µl
ApplicationsW, IF-IC, FC-FP, ChIP, ChIP-seq, C&R, C&T
ReactivityH, M, R, Mk
IsotypeIgG
HostRabbit
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight17
TargetH3 (Lys4) methylate
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) KMT2D depletion promotes KRAS-induced pancreatic carcinogenesis independent of TP53Journal of Biological ChemistryJuly 1, 2026Ezequiel J. Tolosa, Glancis L. Raja Arul, Ashley N. Sigafoos, Victoria Ferrero, David R. Pease, Luciana L. Almada, Renzo E. Vera, Nicole M. Pena Ruiz, Tara L. Hogenson, Kayla C. LaRue-Nolan, et al.Cell signaling), H3K4me3 (ab8580, abcam), H3 (3638S, Cell Signaling), H3K27ac (ab4729, abcam), H3K4me1(5326S, Cell signaling). Blots were then incubated with anti-rabbit (ALI3403, Biosource, 1∶2000 dilution) or

(2) Moderate iron restriction improves metabolism via epigenetic regulation of GDF15The Journal of Nutritional BiochemistryJune 13, 2026Jinying Yang, Limin Shi, Anna L. Cubito, Akshaya Govarthanan, Jian S. Sabripour, Karen Scott, Annette de Kloet, Feng Yue, Bin Liu, Larissa J. Strath, et al.(4056s), Tri-Methyl-Histone H3K4 (9751s), Di-Methyl-Histone H3K4 (9725s), Mono-Methyl-Histone H3K4 (5326s), and α-tubulin (2125s) antibodies from Cell Signaling Technology (Beverly, MA, USA); antibody against

(3) CEBPG‐Mediated Palmitic Acid Adaptation of Cancer‐Associated Fibroblasts Drives Metastasis of Oral Squamous Cell CarcinomaAdvanced ScienceJune 2, 2026Yiling Duan, Yitong Li, Yufei Wu, Xiao Yang, Rui Li, Hui Zhao, Zhengjun Shangextracted according to the manufacturer's protocol. The primary antibodies used were H3K4me1 (CST, 5326), H3K27ac (CST, 8173), or IgG (CST, 2729). Secondary antibody used was Goat Anti‐Rabbit IgG (H+L) antibody

(4) Kdm6b-mediated epigenetic coordination of temporal precision during motor neuron differentiationEMBO ReportsMay 26, 2026Haihan Ren, Qicheng Liu, Bingqian Li, Xuetong Yu, Dan Liang, Shuangling Li, Bing Pan, Qian Gao, Wenhong Deng, Jun-Ma Yu, et al.anti-H3K27me3 (Abcam Ab6002), anti-H3K27ac (Abcam Ab4729), anti-H3K4me1 (Cell Signaling Technology #5326) and anti-H3K4me3 (Abcam Ab8580). Bioinformatic analyses of Cut&Run-seq and Cut&Tag-seq Paired-end

(5) The PLAGL1-KLF4-IRX5 axis promotes the osteogenesis of periosteal progenitors during mandible regenerationNature CommunicationsMay 21, 2026Enhui Yao, Yili Liu, Jingyi Xu, Zeqian Xu, Yilei Huang, Mingliang Zhou, Sihan Lin, Xinquan Jiang, Jiahui Dufollowed by overnight incubation at 4 °C with specific primary antibodies: H3K4me3 (Cell Signaling, 5326, 1:100), HA (Cell Signaling, 3724, 1:100), FLAG (Cell Signaling, 14793, 1:100). Normal rabbit IgG (Cell Signaling

(6) Genotype epigenome phenotype integration reveals peripheral immune contributions to type I bipolar disorderNature CommunicationsMay 15, 2026Lei Hou, Yue Li, Xushen Xiong, Yosuke Tanigawa, Yongjin Park, Samuel W. Lenz, Amy Grayson, Jeong-Heon Lee, Euijung Ryu, Janet E. Olson, et al.antibodies were used in the experiment; anti-H3K27ac (CST #8173), anti-H3K4me1 (Abcam ab8895; CST #5326), in-house generated anti-H3K4me3 (EDL lot 1)137, anti-H3K27me3 (CST #9733), and anti-H3K36me3 (Active Motif

(7) Loss of Kmt2c/d promotes gastric cancer and confers vulnerability to mTORC1 and PD-1 inhibitionJ Clin InvestMay 12, 2026Naitao Wang, Dan Li, Tao Zhang, Mohini R. Pachai, Dana M. Schoeps, Yudi Bao, Woo Hyun Cho, Makhzuna N. Khudoynazarova, Kae Kristoff, Marion Liu, et al.performed ChIP-seq of H3K4me1 and H3K4me3. In each experiment, we used 1 μg antibodies against H3K4me1 (5326, clone D1A9, Cell Signaling Technology) or H3K4me3 (130060, EpiCypher). The library preparation and

(8) CoCUT&Tag maps linked chromatin states at single-molecule, single-cell resolutionbioRxivMay 5, 2026Wenjie Sun, Emma Baird, Harry J. Ashbaugh, Alexandria P. Eiken, Derek H. Janssensanti-H3K4me2 (Diagenode; cat. no. C15200151), rabbit anti-H3K4me1 (Cell Signaling Technologies; cat. no. 5326), mouse anti-H3K27ac (Diagenode; cat. no. C15200184). In addition, we performed standard CUT&Tag assays

(9) Embryonic stem cell factors DPPA2/4 amplify active H3K4me3–H2AK119ub chromatin domains in non-small cell lung cancerGenes & DevelopmentMay 4, 2026Janith A. Seneviratne, Clare L. Crisp, Eleanor Glancy, Natalie Choy, Winnie Tan, Matthew Neve, Melanie Stammers, Tongtong Wang, Ruby Johnstone, Arshnoor Kaur, et al.0.5 µg of H2AK119ub (Cell Signaling Technology 8240), 0.5 µg of H3K4me1 (Cell Signaling Technology 5326T), 1 µg of H3K27ac (Abcam ab4729), 1 µg of H3K9me3 (Abcam ab8898), 0.5 µg of H3K36me2 (Abcam ab9049),

(10) Super‐Enhancer Formation in Scirrhous Gastric CAFs , and the Presence of a Stromal Field in Non‐Cancerous TissuesCancer ScienceMay 3, 2026Yoshimi Yasukawa, Naoko Hattori, Yu‐Yu Liu, Masayuki Tobo, Genki Yamagishi, Takahiro Irie, Shigeki Sekine, Tohru Kiyono, Satoshi Yamashita, Yukinori Yamagata, et al.MA). 10 μg of sonicated chromatin was incubated overnight at 4°C with 10 μL of anti‐H3K4me1 antibody (#5326, Cell Signaling Technology, Danvers, MA) and 10 μL of anti‐H3K4me3 antibody. 20 ng of Spike‐in chromatin

(11) Nucleation-dependent propagation of Polycomb modifications emerges during the Drosophila maternal to zygotic transitionbioRxivApril 11, 2026Natalie Gonzaga-Saavedra, Eleanor A. Degen, Isabella V. Soluri, Corinne Croslyn, Shelby A. Blytherepeat YSPTSPS (phospho S5),abcam,ab5131,ChIP-seq Mono-Methyl-Histone H3 (Lys4) (D1A9),Cell signaling,5326T,ChIP-seq "H3K4me2 Recombinant Superclonal Antibody, ChIP-Verified",Fisher,710796,ChIP-seq TriMethyl-Histone H3-K4 Rabbit pAb

(12) HDAC Inhibition Induces Transient Phenotypic Inertia in Dormant OCCC Spheroids by Derepression of Cell Cycle GenesCellsApril 10, 2026Sylvia Cheng, Bart Kolendowski, Yudith Ramos-Valdes, Trevor G. Shepherd, Gabriel E. DiMattia,Cell Signaling Technology,8173 H3K27me3,Cell Signaling Technology,9733 H3K4me1,Cell Signaling Technology,5326 H3K4me2,Cell Signaling Technology,9725 H3K4me3,Cell Signaling Technology,9751 Histone H3,Cell Signaling Technology

(13) The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathwayTranslational OncologyApril 1, 2026Chunli Wang, Dandan Shen, Nanshan Lin, Panjie Wang, Yaping Bi, Huina Hu, Yujia Shang, Chenchen Ren, Li Yang, Qisheng MaAbcam, UK), H3(1:20,000, No.4499T,Cell Signaling Technology, CST, Boston, MA, USA), H3K4me1(1:2000, No.5326T, CST), H3K4me2(1:2000, No.9725S, CST), H3K4me3(1:2000, No. 9751T, CST), p62/SQSTM1(1:2000, No.5114T,

(14) Hat1 Orchestrates Heterochromatin Inheritance by Regulating Localization of H3K9 MethyltransferasesbioRxivMarch 25, 2026Caden J. Martin, Liudmila V. Popova, Prabakaran Nagarajan, Elizabeth A. Oser, Callie M. Lovejoy, Benjamin D. Sunkel, Benjamin Z. Stanton, Michael A. Freitas, Mark R. ParthunWestern Blot" H3K9me3,Abcam,ab8898,CUT&Tag H3K9me2,Abcam,ab1220,CUT&Tag H3K27me3,Cell Signaling,9733,CUT&Tag H3K4me1,Cell Signaling,5326,CUT&Tag H3K9ac,Cell Signaling,9649,CUT&Tag H3K14ac,Abcam,ab52946,CUT&Tag H3K27ac,Cell Signaling,8173,CUT&Tag H4K5ac,Abcam,ab51997,CUT&Tag H4K12ac,Abcam,ab46983,CUT&Tag Lamin

(15) Respiratory viral infections prime accelerated lung cancer growthCellMarch 11, 2026Wei Qian, Xiaoqin Wei, Andrew J. Barros, Xiangyu Ye, Haibo Zhang, Qing Yu, Samuel P. Young, Eric V. Yeatts, Yury Park, Chaofan Li, et al.8173S Mono-Methyl-Histone H3 (Lys4) (D1A9) Rabbit Monoclonal Antibody,Cell Signaling Technology,Cat# 5326S "Goat anti-Mouse IgG Antibody, Alexa Fluor 647",Invitrogen,Cat# A-21235; RRID AB_2535804 "Goat anti-Rabbit

(16) Mutant Isocitrate Dehydrogenase 1 Sensitizes Intrahepatic Cholangiocarcinoma Cells to MDM2 InhibitorsCancer Res CommunMarch 1, 2026Chien-Tsung Liu, Yung-Yeh Su, Nai-Jung Chiang, Chien-Feng Li, Yu-Chun Ma, Kung-Chao Chang, Yu-Hsuan Hung, Wen-Chun Hung, Li-Tzong ChenRRID:AB_1550038), Mono-Methyl-Histone H3 (Lys4) (D1A9) XP Rabbit mAb (Cell Signaling Technology cat. # 5326, RRID:AB_10695148), Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb (Cell Signaling Technology cat.

(17) Mutant Isocitrate dehydrogenase 1 sensitizes intrahepatic cholangiocarcinoma cells to MDM2 inhibitorsCancer Research CommunicationsMarch 1, 2026Chien-Tsung Liu, Yung-Yeh Su, Nai-Jung Chiang, Chien-Feng Li, Yu-Chun Ma, Kung-Chao Chang, Yu-Hsuan Hung, Wen-Chun Hung, Li-Tzong ChenRRID:AB_1550038), Mono-Methyl-Histone H3 (Lys4) (D1A9) XP Rabbit mAb (Cell Signaling Technology cat. # 5326, RRID:AB_10695148), Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb (Cell Signaling Technology cat.

(18) Oridonin as a novel KDM5C inhibitor alleviates clonal hematopoiesis-induced cardiac aging via H3K4me3-dependent SASP suppressionActa Pharmaceutica Sinica BFebruary 10, 2026Xingling Chen, Qiangqiang Zhao, Linhuang Chen, Shengrong Lin, Bin Mai, Xingling He, Xiaojiao Zhang, Jiahui Chen, Sijing Li, Ziru Li, et al.histone modifications using antibodies against H3K4me3 (Cell Signaling Technology, Danvers, MA, USA; #5326 and #9751). Cells were crosslinked with 1% formaldehyde for 10 min at room temperature and quenched

(19) Epigenetic and Transcriptional Programs Define Osteosarcoma Subtypes and Establish Targetable VulnerabilitiesCancer DiscoveryFebruary 6, 2026Eunice López-Fuentes, Andrew S. Clugston, Alex G. Lee, Leanne C. Sayles, Natalie Sorensen, Maria V. Pons Ventura, Stanley G. Leung, Truc Dinh, Marcus R. Breese, E. Alejandro Sweet-Cordero(Cell Signaling Technology, #8173, RRID: AB_10949503), mono-methyl-histone H3 (Lys4) antibody (Cell Signaling Technology, #5326, RRID: AB_10695148), tri-methyl-histone H3 (Lys4) antibody (Cell Signaling Technology, #9751, RRID:

(20) EpiBrain: the brain’s epigenetic landscape in a snapshotbioRxivJanuary 28, 2026Maja Johnson, Aiden F Eno, Karl Stuntzner-Gibson, James W MacDonald, Theo K Bammler, Yijie Geng(CST#8173), H3K36me3 (CST#4909), H3K9me2 (CST#4658), H3K9ac (CST#9649), H3K4me1 (CST#9751), H3K4me3 (CST#5326), and 5mC (CST#28692), and mouse monoclonal antibodies against histone H3 (CST#14269) and 5hmC (CST#51660).

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Mono-Methyl-Histone H3 (Lys4) (D1A9) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Mono-Methyl-Histone H3 (Lys4) (D1A9) XP ® Rabbit mAb from Cell Signaling Technology

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