Rat IgG2b kappa APC Isotype Control 17-4031-82 from Thermo Fisher Scientific

Product Specs
ItemRat IgG2b kappa APC Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number17-4031-82
Quantity100 µg
ApplicationsControl, Flow Cytometry
Concentration0.2 mg/mL
Conjugate/TagAPC
FormatPBS with 0.09% sodium azide; pH 7.2
TargetRat IgG2b kappa
IsotypeIgG2b kappa
CloneeB149/10H5
HostRat
PurityAffinity chromatography
Storage Temperature4° C, store in dark, DO NOT FREEZE!
Antibody TypeIsotype Control
Storage4°C, store in dark, DO NOT FREEZE!
ClonalityMonoclonal

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United States

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Website: www.thermofisher.com

(1) An IFN-STAT1-CYBB Axis Defines Protective Plasmacytoid DC to Neutrophil Crosstalk During Aspergillus fumigatus InfectionbioRxivDecember 5, 2024Yahui Guo, Mariano A. Aufiero, Kathleen A.M. Mills, Simon A. Grassmann, Hyunu Kim, Paul Zumbo, Mergim Gjonbalaj, Audrey Billips, Katrina B. Mar, Yao Yu, et al.Control,eBioscience,Cat#45-4888-80; RRID: AB_906260 APC Rat IgG2b kappa Isotype Control,eBioscience,Cat#17-4031-82; RRID: AB_470176 APC-eFluor 780 Rat IgG2b kappa Isotype Control,eBioscience,Cat#47-4031-80; RRID: AB_1272021 Alexa

(2) High mitochondrial DNA content is a key determinant of stemness, proliferation, cell migration, and cancer metastasis in vivoCell death & diseaseOctober 11, 2024Mauro-Lizcano M, Di Pisa F, Larrea Murillo L, Sugden CJ, Sotgia F, Lisanti MP.#17-0441-83) or the isotype antibody control (Rat IgG2b kappa Isotype Control, APC, Thermo-Fisher Scientific, #17-4031-82) for 30 min on ice after sorting. Cells were washed and resuspended in 1% BSA-PBS, and analysed by flow cytometry

(3) The choroid plexus synergizes with immune cells during neuroinflammationCellSeptember 5, 2024Xu H, Lotfy P, Gelb S, Pragana A, Hehnly C, Byer LIJ, Shipley FB, Zawadzki ME, Cui J, Deng L, et al.antibody (For VLA-4α, BioLegend 103701, 1μg/mouse), rat IgG2b κ control antibody (ThermoFisher Scientific, 17-4031-82, matching dose to other antibodies). Live and heat-killed S. agalactiae (GBS) were also delivered by

(4) Different depths of food restriction and high‐fat diet refeeding in mice impact host obesity and metabolic phenotypes with correlative changes in the gut microbiotaMedComm (2020)August 1, 2024Jiaqi Xu, Huangru Xu, Feiyan Yang, Yawen Xie, Fangfang Cai, Siyu Mao, Min Lu, Hongqin Zhuang, Zi‐Chun HuaThermo Fisher provided PerCP‐conjugated anti‐CD8+ (Product # MCD0831), anti‐mouse CD11b+ APC (Product # 17‐4031‐82), and FITC anti‐mouse Ly6G+ (Product # 11‐4031‐85) for FACS. A flow cytometer (FACS Calibur; BD Biosciences)

(5) In vitro aging alters the gene expression and secretome composition of canine adipose-derived mesenchymal stem cellsFrontiers in veterinary scienceJanuary 1, 2024Prišlin M, Butorac A, Bertoša R, Kunić V, Ljolje I, Kostešić P, Vlahović D, Naletilić Š, Turk N, Brnić D.,Thermo Fisher Scientific/17-5900-42 "Isotype IgG2b, kappa",eB149/10H5,Rat,APC,N/A,Monoclonal,Thermo Fisher Scientific/17-4031-82 CD44,MEM-263,Mouse,FITC,"Canine, Human, Porcine",Monoclonal,Antibodies-online/ABIN452099 Isotype IgG1,VI-AP,Mouse,FITC,N/A,Monoclonal,Antibodies-online/ABIN1741583 CD105,SN6,Mouse,PE-Cy

(6) The polarization of microglia and infiltrated macrophages in the injured mice spinal cords: a dynamic analysisPeerJJanuary 1, 2023Li JL, Fu GQ, Wang YY, Bian MM, Xu YM, Zhang L, Chen YQ, Zhang N, Ding SQ, Wang R, et al.monoclonal,17-0451-82,AB_469392,APC,0.125 µg/test Invitrogen,FCM,APC,0.125 µg/test,IgG2b kappa Isotype Control,Rat,17-4031-82,AB_470176 Antibodies used in the study.

(7) Stimulation of C-Kit(+) Retinal Progenitor Cells by Stem Cell Factor Confers Protection Against Retinal DegenerationFrontiers in pharmacologyJanuary 1, 2022Chen X, Li S, Liu X, Zhao J, Wu L, You R, Wang Y.Cat# 17-1172-83; eBioscience, Thermo Fisher Scientific, United States) or isotype control (1:50; Cat# 17-4031-82; eBioscience) for 30 min at 4°C. After each procedure, cells were rinsed with staining buffer (eBioscience).

(8) CD95/Fas protects triple negative breast cancer from anti-tumor activity of NK cellsiScienceNovember 19, 2021Qadir AS, Guégan JP, Ginestier C, Chaibi A, Bessede A, Charafe-Jauffret E, Macario M, Lavoué V, Rouge TM, Law C, et al.Reagents used for flow cytometry, anti-CD44-APC conjugated (17-0441-82), Isotype Rat IgG2b control (17-4031-82), PE conjugated-anti-CD95 (2-0951-83) and PE conjugated Isotype control (12-4714-82) were from eBioscience.

(9) Use of a Self-Delivering Anti-CCL3 FANA Oligonucleotide as an Innovative Approach to Target Inflammation after Spinal Cord InjuryeNeuroMarch 1, 2021Pelisch N, Rosas Almanza J, Stehlik KE, Aperi BV, Kroner A.incubation with 5% bovine serum albumin (BSA), followed by staining with antibodies against CD11b (1:500, 17-4031-82, clone M1/70, rat anti-mouse, APC, Thermo Fisher Scientific) and CD45 (1:500, 25-0451-82, clone 30-F11,

(10) An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogoniaCell reportsFebruary 16, 2021Suzuki S, McCarrey JR, Hermann BP.kappa Isotype Ctrl Antibody (Clone eB149/10H5; 4μg/ml); Figure S3,ThermoFisher,RRID: AB_470176 Cat# 17-4031-82 Alexa Flour 700-conjugated Rat IgG2b kappa Isotype Ctrl Antibody (Clone RTK4530; 2μg/ml); Figure S3,Biolegend,RRID:

(11) Icariin Alleviates Wear Particle-Induced Periprosthetic Osteolysis via Down-Regulation of the Estrogen Receptor α-mediated NF-κB Signaling Pathway in MacrophagesFrontiers in pharmacologyJanuary 1, 2021Guangtao F, Zhenkang W, Zhantao D, Mengyuan L, Qingtian L, Yuanchen M, Yuanfeng C, Qiujian Z.25-5920-82, Thermo Fisher Scientific). The isotype controls used were APC-conjugated rat IgG2b, κ (17-4031-82, Thermo Fisher Scientific) and PE-cyanine7-conjugated rat IgG2a, κ (25-4321-82, Thermo Fisher Scientific). SiRNA

(12) High-fat diet-induced obesity affects alpha 7 nicotine acetylcholine receptor expressions in mouse lung myeloid cellsScientific reportsOctober 27, 2020Qi Y, Si D, Zhu L, Qi Y, Wu Z, Chen D, Yang Y.company. PerCP-Cy5.5 rat anti-mouse CD45 (45-0451-82, 1:100), APC rat anti-mouse IgG2b κ isotype control (17-4031-82, 1.25:100) and APC rat anti-mouse CD206 (17-2061-82, 1.25:100) were purchased from eBioscience. The

(13) CRID3, a blocker of apoptosis associated speck like protein containing a card, ameliorates murine spinal cord injury by improving local immune microenvironmentJournal of neuroinflammationAugust 29, 2020Chen YQ, Wang SN, Shi YJ, Chen J, Ding SQ, Tang J, Shen L, Wang R, Ding H, Hu JG, et al.μg/test Invitrogen,FCM,CD45,Rat monoclonal,17-0451-82,AB_469392,APC,0.125 μg/test Invitrogen,FCM,IgG2b kappa isotype control,Rat,17-4031-82,AB_470176,APC,0.125 μg/test Table of antibodies used

(14) During Aspergillus Infection, Monocyte-Derived DCs, Neutrophils, and Plasmacytoid DCs Enhance Innate Immune Defense through CXCR3-Dependent CrosstalkCell host & microbeJuly 8, 2020Guo Y, Kasahara S, Jhingran A, Tosini NL, Zhai B, Aufiero MA, Mills KAM, Gjonbalaj M, Espinosa V, Rivera A, et al.Control,eBioscience,Cat# 45-4888-80; RRID: AB_906260 APC Rat IgG2b kappa Isotype Control,eBioscience,Cat# 17-4031-82; RRID: AB_470176 APC-eFluor 780 Rat IgG2b kappa Isotype Control,eBioscience,Cat# 47-4031-80; RRID:

(15) TGF-β-driven downregulation of the transcription factor TCF7L2 affects Wnt/β-catenin signaling in PDGFRα⁺ fibroblastsJournal of cell scienceJune 19, 2020Contreras O, Soliman H, Theret M, Rossi FMV, Brandan E.FITC, clone G155-178, Cat. no. 553456, BD, 1:100; rat anti-IgG2b κ, APC, clone eB149/10H5, Cat. no. 17-4031-82, eBioscience, 1:100). To assess viability, cells were stained with propidium iodide (1 μg ml–1) and

(16) ZBTB20-mediated titanium particle-induced peri-implant osteolysis by promoting macrophage inflammatory responsesBiomaterials scienceJune 7, 2020Qiu J, Peng P, Xin M, Wen Z, Chen Z, Lin S, Kuang M, Fu Y, Fang G, Li S, et al.Thermo Fisher Scientific) at 20 °C for 20 min in the dark. The isotype controls used were APC-conjugated rat IgG2b,κ (17-4031-82, Thermo Fisher Scientific) and PE-cyanine7-conjugated rat IgG2a,κ (25-4321-82, Thermo Fisher Scientific).

(17) Mutations in the KIF21B kinesin gene cause neurodevelopmental disorders through imbalanced canonical motor activityNature communicationsMay 15, 2020Asselin L, Rivera Alvarez J, Heide S, Bonnet CS, Tilly P, Vitet H, Weber C, Bacino CA, Baranaño K, Chassevent A, et al.stained with the Rat IgG2b-APC isotype control antibody (clone eB149/10H5, ThermoFisher Scientific (#17-4031-82)) and used as controls to set the YFP and CD24 gates. Microfluidic fabrication and neurons plating Design

(18) Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survivalProtein & cellFebruary 1, 2020Tong D, Zhang L, Ning F, Xu Y, Hu X, Shi Y.IgG2b K isotype control PE (eBioscience, 12-4031-82), rat IgG2b k isotype control APC (eBioscience, 17-4031-82), rat IgG2b k isotype control FITC (eBioscience, 11-4031-82), anti-mouse V alpha 2 TCR eFluor 450 (eBioscience,

(19) Cross-talk between TGF-β and PDGFRα signaling pathways regulates the fate of stromal fibro-adipogenic progenitorsJournal of cell scienceOctober 9, 2019Contreras O, Cruz-Soca M, Theret M, Soliman H, Tung LW, Groppa E, Rossi FM, Brandan E.anti-IgG2a k, FITC, clone G155-178, BD, Cat No: 553456; rat IgG2b kappa, APC, clone eB149/10H5, Cat. no. 17-403182 – all from eBioscience). To assess viability, cells were stained with propidium iodide (1 μg ml–1) and

(20) Development of Hematopoietic Stem Cell-Engineered Invariant Natural Killer T Cell Therapy for CancerCell stem cellOctober 3, 2019Zhu Y, Smith DJ, Zhou Y, Li YR, Yu J, Lee D, Wang YC, Di Biase S, Wang X, Hardoy C, et al.MPC-11)",Biolegend,CAT#400320 "Rat lgG2b, κ isotype control antibody (Clone eB149/10H5)",eBioscience,"CAT#17-4031-82, RRID: AB_470176" Human Fc Receptor Blocking Solution (TrueStain FcX),Biolegend,CAT#422302 Mouse Fc

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Rat IgG2b kappa APC Isotype Control from Thermo Fisher Scientific

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Rat IgG2b kappa APC Isotype Control from Thermo Fisher Scientific

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