Rat IgG2a kappa Alexa Fluor 488 Isotype Control 53-4321-80 from Thermo Fisher Scientific

Product Specs
ItemRat IgG2a kappa Alexa Fluor 488 Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number53-4321-80
Quantity25 µg
ApplicationsControl, Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Concentration0.5 mg/mL
Conjugate/TagAlexa Fluor™ 488
FormatPBS with 0.09% sodium azide; pH 7.2
TargetRat IgG2a kappa
IsotypeIgG2a kappa
CloneeBR2a
HostRat
PurityAffinity chromatography
Storage Temperature4° C, store in dark, DO NOT FREEZE!
Antibody TypeIsotype Control
Storage4°C, store in dark, DO NOT FREEZE!
ClonalityMonoclonal

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

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Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Aggressive Cholesterol Lowering Normalizes Atherosclerosis Regression in Jak2V617F MiceJournal of Lipid ResearchFebruary 18, 2026Brian D. Hardaway, Trevor P. Fidler, Mojdeh Tavallaie, Cheng-Chieh Hsu, Sandra Schiavone, Tong Xiao, Ranran Wang, Nan Wang, Alan R. Tall(ChromPure, 012-000-003), Rat IgG (BioLegend, 400431), Rat IgG conjugated to Alexa Fluor 488 (Invitrogen, 53-4321-80). Slides were then washed twice with PBS-T for 5 min and once with 1X PBS for 5 min. The following secondary

(2) Prevention and Reversal of Hypertension‐Induced Coronary Microvascular Dysfunction by a Plant‐Based DietJournal of the American Heart AssociationNovember 18, 2025Rami S. Najjar, Nedumangalam Hekmatyar, Yanling Wang, Vu Ngo, Hannah L. Lail, Juan P. Tejada, Jessica P. Danh, Desiree Wanders, Rafaela G. Feresin, Puja K. Mehta, et al.immunoglobulin G1 κ eF660 (Catalog No. 50‐4301‐82) and immunoglobulin G2a κ isotype AF488 (Catalog No. 53‐4321‐80) or a cocktail of conjugated monoclonal antibodies CD31 (25‐0310‐82; Thermo Fisher Scientific), CD90

(3) Prevention and reversal of hypertension-induced coronary microvascular dysfunction by a plant-based dietbioRxivAugust 6, 2025Rami S. Najjar, Nedumangalam Hekmatyar, Yanling Wang, Vu Ngo, Hannah L. Lail, Juan P. Tejada, Jessica P. Danh, Desiree Wanders, Rafaela G. Feresin, Puja K. Mehta, et al.incubated with isotypes IgG1 kappa eF660 (Cat #: 50–4301-82) and IgG2a kappa isotype AF488 (Cat #: 53–4321-80) or a cocktail of conjugated monoclonal antibodies CD31 (25–0310-82, ThermoFisher Scientific), CD90

(4) Aggressive Cholesterol Lowering Normalizes Atherosclerosis Regression in Jak2 V617F Clonal HematopoiesisbioRxivJuly 31, 2025Brian D. Hardaway, Trevor P. Fidler, Mojdeh Tavallaie, Kleopatra Avrampou, Cheng-Chieh Hsu, Sandra Schiavone, Tong Xiao, Nan Wang, Alan R. Tall(ChromPure, 012-000-003), Rat IgG (Biolegend, 400431), Rat IgG conjugated to Alexa Fluor 488 (Invitrogen, 53-4321-80). Slides were then washed twice with PBS-T for 5 minutes and once with 1X PBS for 5 minutes. The following

(5) Platelets exacerbate cardiovascular inflammation in a murine model of Kawasaki disease vasculitisJCI insightJuly 24, 2023Kocatürk B, Lee Y, Nosaka N, Abe M, Martinon D, Lane ME, Moreira D, Chen S, Fishbein MC, Porritt RA, et al.Tonbo Biosciences, catalog 65-4031-U100), and rat anti-IgG2a–Alexa Fluor 488 (clone eBR2a; eBioscience, catalog 53-4321-80). Stained cells were analyzed on a FACSAria III (BD Biosciences) and the data were processed using FlowJo

(6) MACROPHAGE SWITCHING: POLARIZATION AND MOBILIZATION AFTER TRAUMAShock (Augusta, Ga.)February 1, 2023Hoteit L, Loughran P, Haldeman S, Reiser D, Alsaadi N, Andraska E, Bonaroti J, Srinivasan A, Williamson KM, Alvikas J, et al.137016 iNOS,FITC,Thermofisher 53-5920-82 iNOS isotype control: Rat IgG2a kappa Isotype Control,Alexa Fluor 488,Thermofisher 53-4321-80 Arg1,APC,R&D IC5868A Arg1 isotype control,Sheep IgG,R&D 1C016A

(7) Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosiseLifeMay 10, 2022Teemu P Miettinen, Kevin S Ly, Alice Lam, Scott R Manalisdilution) Antibody,IgG2a kappa Isotype Control conjugated to Alexa Fluor 488 (rat monoclonal),Thermo Fisher Scientific,Cat#53-4321-80; RRID:AB_493963,Clone: eBR2a (1:50 dilution) Antibody,Anti-LAMP-1 conjugated to Alexa Fluor 488 (mouse

(8) CD73-Mediated Immunosuppression Is Linked to a Specific Fibroblast Population That Paves the Way for New Therapy in Breast CancerCancersNovember 23, 2021Magagna I, Gourdin N, Kieffer Y, Licaj M, Mhaidly R, Andre P, Morel A, Vincent-Salomon A, Paturel C, Mechta-Grigoriou F.Thermofisher Scientific, #53-4776) or the corresponding isotype control (1:200, Thermofisher scientific, #53-4321-80) for 30 min at RT. Stained T cells were analyzed by LSRFortessaTM analyzer (BD biosciences). At least

(9) Sex-specific stress response and HMGB1 release in pulmonary endothelial cellsPloS oneJanuary 1, 2020Zemskova M, Kurdyukov S, James J, McClain N, Rafikov R, Rafikova O.(Invitrogen, 53-1021-82) in dilution 1:100 in the dark. Rat IgG2 iso-control antibodies (eBioscience, 53-4321-80) were used as control. MLEC labeled with anti-ICAM-2 and control antibody were washed, resuspended in

(10) The role of microglial inflammasome activation in pyroptotic cell death following penetrating traumatic brain injuryJournal of neuroinflammationFebruary 8, 2019Lee SW, de Rivero Vaccari JP, Truettner JS, Dietrich WD, Keane RW.cells were labeled for surface markers CD45 Alexa 647 (202212, 1.25 μg/mL, BioLegend) and CD11b v450 (53-4321-80, 1 μg/mL, eBioscience). CD45 and CD11b were used to distinguish between CD45low “resting” microglia

(11) Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast CancerCancer cellMarch 12, 2018Costa A, Kieffer Y, Scholer-Dahirel A, Pelon F, Bourachot B, Cardon M, Sirven P, Magagna I, Fuhrmann L, Bernard C, et al.

(12) A negative allosteric modulator of PDE4D enhances learning after traumatic brain injuryNeurobiology of learning and memoryFebruary 1, 2018Titus DJ, Wilson NM, Alcazar O, Calixte DA, Dietrich WD, Gurney ME, Atkins CM.labeled with CD45 Alexa 647 (1.25 μg/ml, 202212, BioLegend, San Diego, CA, USA) and CD11b v450 (1 μg/ml, 53-4321-80, eBioscience, Waltham, MA, USA). The surface markers CD45 and CD11b were used to distinguish microglia

(13) Therapeutic benefits of phosphodiesterase 4B inhibition after traumatic brain injuryPloS oneJanuary 1, 2017Wilson NM, Gurney ME, Dietrich WD, Atkins CM.cells were labeled for surface markers CD45 Alexa 647 (202212, 1.25 μg/ml, BioLegend) and CD11b v450 (53-4321-80, 1 μg/ml, eBioscience). The surface markers CD45 and CD11b were used to distinguish between CD45low

(14) Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the HippocampusFrontiers in systems neuroscienceJanuary 1, 2016Wilson NM, Titus DJ, Oliva AA Jr, Furones C, Atkins CM.suspension and cells were labeled with CD45 Alexa 647 (202212, 1.25 μg/ml, BioLegend) and CD11b v450 (53-4321-80, 1 μg/ml, eBioscience). Dead cells were excluded using LIVE/DEAD Fixable NearIR dead cell stain (L10119,

(15) Upregulation of Programmed Death-1 and Its Ligand in Cardiac Injury Models: Interaction with GADD153PloS oneJanuary 1, 2015Baban B, Liu JY, Qin X, Weintraub NL, Mozaffari MS.

(16) The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammationNature immunologyAugust 1, 2014Franklin BS, Bossaller L, De Nardo D, Ratter JM, Stutz A, Engels G, Brenker C, Nordhoff M, Mirandola SR, Al-Amoudi A, et al.

(17) The CD4(+) T-cell response of melanoma patients to a MAGE-A3 peptide vaccine involves potential regulatory T cellsCancer researchMay 15, 2009François V, Ottaviani S, Renkvist N, Stockis J, Schuler G, Thielemans K, Colau D, Marchand M, Boon T, Lucas S, et al.

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Rat IgG2a kappa Alexa Fluor 488 Isotype Control from Thermo Fisher Scientific

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Rat IgG2a kappa Alexa Fluor 488 Isotype Control from Thermo Fisher Scientific

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