CD3 Antibody Functional Grade 16-0032-82 from Thermo Fisher Scientific

Product Specs
ItemCD3 Antibody Functional Grade
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number16-0032-82
Quantity100 µg
ApplicationsFlow Cytometry, Functional assay
Concentration1 mg/mL
Conjugate/TagFunctional Grade
FormatPBS with no preservative; pH 7.2
ReactivityMouse
TargetCD3
IsotypeIgG2b kappa
Clone17A2
HostRat
NCBI Full Gene NameCD3 antigen, delta polypeptide, CD247 antigen, CD3 antigen, gamma polypeptide, CD3 antigen, epsilon polypeptide
NCBI Gene AliasesCD3epsilon, Ctg-3, Cd3z, Cd3-zeta, Cd3-eta, Ctg3, T3g, T3d, Cd3, Tcrk, Cd3h, Tcrz, T3z, Cd3zeta, CD3, T3e, 4930549J05Rik, A430104F18Rik
ClonalityMonoclonal
PurityAffinity chromatography
Storage Temperature4° C
Antibody TypePrimary
Storage4°C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Preoperative exercise induces anti-tumor Kupffer cells to prevent surgical stress-promoted colorectal cancer liver metastasisCell Reports MedicineFebruary 17, 2026Yunwei Zhang, Yiyu Zhang, Chengli Shen, Jiye Li, Han Wang, Ahmad Hamad, Chunyan Cao, Mohamad El Moheb, Yu Wang, Yujia Xia, et al.,BD Biosciences,Cat# 749569; RRID: AB_2873894 CD3 Monoclonal Antibody (Clone: 17A2),Thermo Fisher Scientific,"Cat# 16-0032-82, RRID: AB_468851" CD28 Monoclonal Antibody (Clone: 37.51),Thermo Fisher Scientific,"Cat# 16-0281-82,

(2) Activated T-cell membrane-derived nanocargoes displaying multi-immune checkpoints for enhanced cancer immunotherapyMaterials Today BioFebruary 1, 2026Li Du, Xiaoying Zhang, Yao Gong, Miaoshu Liu, Jide Sun, Xingping Hu, Jian Peng, Zhangling Liu, Ting Zhang, Jie Xu, et al.Poly HRP) were used for Immunofluorescence Analysis. CD3 Monoclonal Antibody (ThermoFisher: cat.no.16-0032-82) and CD28 Monoclonal Antibody (ThermoFisher: cat.no.16-0081-82) were used for T cells activation and

(3) Integrated scRNA-seq and transcriptome analyses uncover the effects of UBE2H on the immune microenvironment regulation in pancreatic cancerCancer Cell InternationalNovember 24, 2025Fuxin Huang, Zhongyan Zhang, Jike Fang, Yue Chen, Jinhui Wei, Quanzhang Li, Chuanzhao Zhang, Shanzhou Huang, Baohua HouIgG H&L (PE) preadsorbed,abcam,ab72465 "CD3 Monoclonal Antibody (17A2), Functional Grade",Invitrogen,16–0032−82 "CD28 Monoclonal Antibody (37.51), Functional Grade",Invitrogen,16–0281−82 BamHI-HF,NEB,R3136V EcoRI-HF,NEB,R3101 ClonExpress

(4) Macrophages foster anti-tumor immunity by ZEB1-dependent cytotoxic T cell chemoattractionCommun BiolJuly 1, 2025Kathrin Fuchs, Elisabetta D'Avanzo, Isabell Armstark, Ruthger van Roey, Ana Clavel Ezquerra, Nino Bindel, Katharina Siebenkäs, Yussuf Hajjaj, Renato Liguori, Fulvia Ferrazzi, et al.Claret Labeling following manufacturer´s instructions. 3 μg/ml anti-CD3 (Thermo Fisher Scientific, 16-0032-82) and 5 μg/ ml anti-CD28 (Thermo Fisher Scientific, 16-0281-82) antibodies were added to 2 × 106 T cells/

(5) FcRn-silencing of IL-12Fc prevents toxicity of local IL-12 therapy and prolongs survival in experimental glioblastomaNature communicationsMay 22, 2025Beffinger M, Schellhammer L, Taskoparan B, Deplazes S, Salazar U, Tatari N, Seehusen F, von Balthazar L, Zinner CP, Spath S, et al.cat. AB-0600) at 100,000 cells per well and stimulated with anti-CD3ε (Thermo Fischer Scientific, cat. 16-0032-82) and anti-CD28 (Thermo Fischer Scientific, cat. 16-0281-82), at a concentration of 1 µg/mL each. IL-12

(6) β-Arrestin 2 as a Prognostic Indicator and Immunomodulatory Factor in Multiple MyelomaCellsMarch 26, 2025Parker Mathews, Xiaobei Wang, Jian Wu, Shaima Jabbar, Kimberly Burcher, Lindsay Rein, Yubin KangThe following antibodies were purchased from commercial sources as indicated: anti-mouse CD3 (Cat. #16-0032-82) and anti-mouse CD28 (Cat. #16-0281-82) from Thermo Fisher, Waltham, MA, USA; anti-mouse CD3-PE (Cat.

(7) Adaptive immune cells antagonize ILC2 homeostasis via SLAMF3 and SLAMF5Sci AdvJanuary 10, 2025Yuande Wang, Dan Li, Yehai Liu, Shasha Chen, Zhongjun DongAnti-CD19 (Cell Signaling Technology, no. 3574; IF, 1:200), anti-CD3 (Thermo Fisher Scientific, no. 16-0032-82; IF, 1:200), anti-rat IgG (H+L) (Thermo Fisher Scientific, no. A11006; IF, 1:200), anti-rabbit IgG (H+L)

(8) Statins inhibit paclitaxel-induced PD-L1 expression and increase CD8+ T cytotoxicity for better prognosis in breast cancerInternational journal of surgery (London, England)August 1, 2024Li L, Wang H, Zhang S, Gao S, Lu X, Pan Y, Tang W, Huang R, Qiao K, Ning S.,Thermo Fisher Scientific,Cat# 16-0037-81 RRID:AB_468854 CD3 Monoclonal Antibody,Thermo Fisher Scientific,Cat# 16-0032-82 RRID:AB_468851 CD28 Monoclonal Antibody,Thermo Fisher Scientific,Cat# 16-0289-81 RRID:AB_468926 CD28

(9) Improvement of Tumor Neoantigen Detection by High-Field Asymmetric Waveform Ion Mobility Mass SpectrometryCancer immunology researchAugust 1, 2024Meng W, Takeuchi Y, Ward JP, Sultan H, Arthur CD, Mardis ER, Artyomov MN, Lichti CF, Schreiber RD.C2836). The following mAbs were used for in vitro T-cell stimulation, anti-CD3 (17A2, ThermoFisher, Cat# 16-0032-82) and anti-CD28 (37.51, ThermoFisher, Cat# 16-0281-82). The following mAbs were used for flow cytometry:

(10) Improvement of Tumor Neoantigen Detection by High-Field Asymmetric Waveform Ion Mobility Mass SpectrometryCancer Immunology ResearchAugust 1, 2024Wei Meng, Yoshiko Takeuchi, Jeffrey P. Ward, Hussein Sultan, Cora D. Arthur, Elaine R. Mardis, Maxim N. Artyomov, Cheryl F. Lichti, Robert D. SchreiberC2836). The following mAbs were used for in vitro T-cell stimulation, anti-CD3 (17A2, ThermoFisher, Cat# 16-0032-82) and anti-CD28 (37.51, ThermoFisher, Cat# 16-0281-82). The following mAbs were used for flow cytometry:

(11) Artesunate induces melanoma cell ferroptosis and augments antitumor immunity through targeting Ido1Cell communication and signaling : CCSJuly 26, 2024Liu W, Zhou H, Lai W, Hu C, Wang Q, Yuan C, Luo C, Yang M, Hu M, Zhang R, et al.

(12) Activation of STING in pancreatic cancer-associated fibroblasts exerts an antitumor effect by enhancing tumor immunityScientific reportsJuly 24, 2024Suzuki Y, Sato T, Sugimori M, Kanemaru Y, Onodera S, Tsuchiya H, Nakamori Y, Tsuyuki S, Ikeda A, Ikeda R, et al.functional CD28 antibody (eBioscience™ #16-0281-82) and 1 μg/mL functional CD3 antibody (eBioscience™ #16-0032-82) for 3 days. To collect murine peritoneal macrophages, thioglycolate medium (Nissui) was injected into

(13) An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndromeDisease models & mechanismsJuly 1, 2024Maino E, Scott O, Rizvi SZ, Chan WS, Visuvanathan S, Zablah YB, Li H, Sengar AS, Salter MW, Jia Z, et al.cells/ml) and stimulated with anti-CD3 monoclonal antibody (17A2) (1 μg/ml, Thermo Fisher Scientific, 16-0032-82), anti-CD28 monoclonal antibody (37.51) (1 μg/ml, Thermo Fisher Scientific, 16-0281-82) and recombinant

(14) Ablation of Atp5if1 impairs metabolic reprogramming and proliferation of T lymphocytes and compromises mouse survivaliScienceJune 21, 2024Romero-Carramiñana I, Dominguez-Zorita S, Esparza-Moltó PB, Cuezva JM.

(15) SLAM-family receptors promote resolution of ILC2-mediated inflammationNature communicationsJune 13, 2024Wang Y, Quan Y, He J, Chen S, Dong Z.The images were analyzed with Zeiss software. Anti-KLRG1 (Thermo, #16-5893-82), anti-CD3 (Thermo, #16-0032-82), anti-B220 (Thermo, #12-0452-82), anti-Syrian Hamster IgG (H + L) (Thermo, #A21451), anti-Rat IgG (H

(16) Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistanceAutophagyJune 1, 2024Zhang X, Lao M, Yang H, Sun K, Dong Y, He L, Jiang X, Wu H, Jiang Y, Li M, et al.

(17) The regulatory relationship between NAMPT and PD-L1 in cancer and identification of a dual-targeting inhibitorEMBO molecular medicineApril 1, 2024Yang Y, Li Z, Wang Y, Gao J, Meng Y, Wang S, Zhao X, Tang C, Yang W, Li Y, et al.

(18) A biphenotypic lymphocyte subset displays both T- and B-cell functionalitiesCommunications biologyJanuary 5, 2024Zhang Y, Guo C, Zhou Y, Zhang W, Zhu Z, Wang W, Wan Y.

(19) Targeting adrenergic receptors to mitigate invariant natural killer T cells-induced acute liver injuryiScienceOctober 20, 2023Gonzatti MB, Freire BM, Antunes MM, de Menezes GB, Talbot J, Peron JPS, Basso AS, Keller AC.AB_467133" "Anti-mouse CD3 Monoclonal Antibody, Functional Grade",eBioscience™,"Clone: 17A2, Catalog # 16-0032-82; RRID: AB_468851" "Anti-mouse CD28 Monoclonal Antibody, Functional Grade",eBioscience™,"Clone: 37.51,

(20) Nanotargeted Delivery of Immune Therapeutics in Type 1 DiabetesAdvanced materials (Deerfield Beach, Fla.)October 1, 2023Jung S, Ben Nasr M, Bahmani B, Usuelli V, Zhao J, Sabiu G, Seelam AJ, Naini SM, Balasubramanian HB, Park Y, et al.desalting column (Thermo Scientific Fisher, Zeba™, 7 kD MWCO). Anti-CD3 mAb (Thermo Fisher Scientific, 16–0032-82) Anti-CD3* mAb, IR800 (IRDye 800CW Carboxylate, LI-COR, 929–08972), or Alexa 594 (Alexa Fluor™ 594 Carboxylic

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CD3 Antibody Functional Grade from Thermo Fisher Scientific

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CD3 Antibody Functional Grade from Thermo Fisher Scientific

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