Mouse IgG1 kappa PE Isotype Control 12-4714-82 from Thermo Fisher Scientific

Product Specs
ItemMouse IgG1 kappa PE Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number12-4714-82
Antibody TypeIsotype Control
Storage4°C, store in dark, DO NOT FREEZE!
ClonalityMonoclonal
Storage Temperature4° C, store in dark, DO NOT FREEZE!
PurityAffinity chromatography
ApplicationsFlow Cytometry (FCM)
HostMouse
CloneP3.6.2.8.1
IsotypeIgG1 kappa
TargetMouse IgG1 kappa
FormatPBS with 0.09% sodium azide; pH 7.2
Conjugate/TagPE
Concentration0.2 mg/mL
Quantity100 µg

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Website: www.thermofisher.com

(1) The PTBP1-EZH2-DOCK2 axis promotes M1 microglial polarization and exacerbates neuronal apoptosis following spinal cord injuryJournal of Orthopaedic TranslationJanuary 22, 2026Chunhe Sha, Feng Pan, Xiaodong Liu, Zhiqing Wang, Hongbin Dai, Kai Huangkappa (Clone eBMG2b; 12-4732-81, Thermo Fisher Scientific), and PE Mouse IgG1 kappa (Clone P3.6.2.8.1; 12-4714-82, Thermo Fisher Scientific). After incubation, cells were stained with 7-AAD (PD00101, Proteintech) for

(2) Antitumor Activity of Death Receptor 5-Targeted Camptothecin-Loaded Nanoparticles in Murine Syngeneic ModelsBiomacromoleculesJanuary 12, 2026Anna J Boland, Michelle K Greene, Úna M Herron, Michael C Johnston, Peter Smyth, Hideo Yagita, Daniel B Longley, Christopher J ScottFACS buffer containing PE-conjugated anti-DR5 (Thermo #12–9908–42, 1:100) or isotype control (Thermo #12–4714–82, 1:200) antibodies and incubated for 30 min on ice in darkness. Cells were then washed as before and

(3) Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in ApoptosisCancers (Basel)September 30, 2025Erin M. Thorpe, Gaëlle Muller-Greven, Jamila Hirbawi, Candece L. Gladson, Michael Kalafatis(Thermo, Cat# 12-9908-42, RRID: AB_10668836), or the mouse IgG1 kappa PE isotype control (Thermo, Cat# 12-4714-82, RRID: AB_470060) were added directly into their respective tubes. All samples were mixed by gentle

(4) Co-Infection by Porphyromonas gingivalis and Fusobacterium nucleatum Increases PD-L1 Expression in Esophageal Cancer TissuesMicrobiologyopenAugust 11, 2025Xiayi Lin, Xiaohui Chen, Mengyuan Chen, Yiqiu Li, Yu Deng, Bohong Xian, Zijun Lianti‐CD274 antibody (eBioscience, 12‐5983‐42, Thermo Fisher Scientific) or PE‐isotype control (eBioscience, 12‐4714‐82, Thermo Fisher Scientific) at 4°C for 40 min in the dark. Staining was performed on at least 10,000

(5) Arjunolic acid inhibits Wnt3a-mediated macrophage M2 polarization to suppress osteosarcoma progressionGenes NutrMay 21, 2025Jun Li, Shuang Zhang, Chao Yu, Xia Chen, Weiye Zhong, Yi Shenanti-CD163 (12–1639-42, eBioscience®), and isotype control antibody (Mouse IgG1κ Isotype Control PE, 12–4714-82, eBioscience®) for 30 min to account for staining. After a final PBS wash, cells were resuspended in

(6) Viral modulation of type II interferon increases T cell adhesion and virus spreadNature CommunicationsJune 22, 2024Carina Jacobsen, Nina Plückebaum, George Ssebyatika, Sarah Beyer, Lucas Mendes-Monteiro, Jiayi Wang, Kai A. Kropp, Víctor González-Motos, Lars Steinbrück, Birgit Ritter, et al.(ImmunoTools #21629233×2) or isotype control antibodies (Biolegend #401606, eBioscience™ (Invitrogen) #12-4714-82), and Zombie-NIR (Biolegend #423106) to quantify cell viability. Afterwards, cells were washed again

(7) Gastrointestinal Dysmotility Predisposes to Colitis through Regulation of Gut Microbial Composition and Linoleic Acid MetabolismAdvanced science (Weinheim, Baden-Wurttemberg, Germany)May 1, 2024Zhang Y, Song F, Yang M, Chen C, Cui J, Xing M, Dai Y, Li M, Cao Y, Lu L, et al.stained with rat anti‐mouse IFNγ (Clone 4S.B3, eBioscience, 12‐7319‐41) or isotype control (eBioscience, 12‐4714‐82) antibody for 30 min at 4 °C according to the BD intracellular staining protocol. BMDM Generation Bone

(8) Glutamine-mediated epigenetic regulation of cFLIP underlies resistance to TRAIL in pancreatic cancerExperimental & molecular medicineApril 1, 2024Kim JH, Lee J, Im SS, Kim B, Kim EY, Min HJ, Heo J, Chang EJ, Choi KC, Shin DM, et al.flow cytometry using the following phycoerythrin-conjugated antibodies: mouse IgG isotype control (12-4714-82, Thermo Fisher), mouse anti-human CD261 (DR4, Biolegend, San Diego, CA, USA), and mouse anti-human CD

(9) Activation of endogenous retroviruses and induction of viral mimicry by MEK1/2 inhibition in pancreatic cancerScience advancesMarch 29, 2024Cortesi A, Gandolfi F, Arco F, Di Chiaro P, Valli E, Polletti S, Noberini R, Gualdrini F, Attanasio S, Citron F, et al.IgG-phycoerythrin (PE) and LDLR-PE], blocked, and stained with IgG1 Isotype Control-PE (eBioscience, 12-4714-82) or anti-human LDLR-PE (BD Pharmingen, 565653). FACS analysis was performed on single-cell suspensions

(10) GATA-3-dependent Gene Transcription is Impaired upon HDAC InhibitionClinical cancer research : an official journal of the American Association for Cancer ResearchMarch 1, 2024Geng X, Wang C, Abdelrahman S, Perera T, Saed B, Hu YS, Wolfe A, Reneau J, Murga-Zamalloa C, Wilcox RA.1 μM HDACi for 8 hours. Cells were stained with 1 μg isotype IgG control (Thermo Fisher Scientific, 12-4714-82) or CCR4 antibody (BD Biosciences, 561110) for 15 min in dark at room temperature. Flow cytometry data

(11) Isolation and characterization of feline endometrial mesenchymal stem cellsJournal of veterinary scienceMarch 1, 2024Park MK, Song KH.kappa",FITC,Dog,Rat,Invitrogen,11-5450-42 Control,IgG2b,FITC,-,Mouse,Invitrogen,PA5-33225 Control,IgG2a,FITC,-,Mouse,Invitrogen,PA5-33239 Control,"IgG1, kappa",PE,-,Mouse,Invitrogen,12-4714-82 Control,"IgG2b, kappa",FITC,-,Rat,Invitrogen,11-4031-81 Antibody information

(12) Human inherited CCR2 deficiency underlies progressive polycystic lung diseaseCellJanuary 18, 2024Neehus AL, Carey B, Landekic M, Panikulam P, Deutsch G, Ogishi M, Arango-Franco CA, Philippot Q, Modaresi M, Mohammadzadeh I, et al.CD163-PE,eBioscience,Cat# 12-1639-42; RRID:AB_1963570 Mouse IgG1 κ Isotype control-PE,eBioscience,Cat# 12-4714-82; RRID:AB_470060 Human CD206-BV421,BioLegend,Cat# 321126; RRID:AB_2563839 Mouse IgG1 κ Isotype control-BV421,BioLegend,Cat#

(13) Comparison of retinal degeneration treatment with four types of different mesenchymal stem cells, human induced pluripotent stem cells and RPE cells in a rat retinal degeneration modelJournal of translational medicineDecember 14, 2023Liu Q, Liu J, Guo M, Sung TC, Wang T, Yu T, Tian Z, Fan G, Wu W, Higuchi A.FITC-labeled isotype (11-471482, Thermo Fisher Scientific, Waltham, MA, USA), and PE-labeled isotype (12-4714-82, Thermo Fisher Scientific, Waltham, MA, USA)] at the recommended concentrations using 5 µL (0.5 µg)/test,

(14) Low level of ARID1A contributes to adaptive immune resistance and sensitizes triple-negative breast cancer to immune checkpoint inhibitorsCancer communications (London, England)September 1, 2023Chen XY, Li B, Wang Y, Jin J, Yang Y, Huang LH, Yang MD, Zhang J, Wang BY, Shao ZM, et al.CD274‐PE (1:20, 12‐5983‐42, eBioscience, San Diego, CA, USA) or mouse IgG1 kappa isotype control‐PE (1:20, 12‐4714‐82, eBioscience). After washing with cold PBS, PD‐L1 expression on the cell surface was detected by flow cytometry

(15) Establishing a novel and sensitive assay for bioactivity determination of anti-CD25 antibodiesHeliyonJune 1, 2023Duan M, Yu C, Yang Y, Fu Z, Liu C, Du J, Li M, Guo S, Yu X, Xu G, et al.anti-phospho-STAT5 (Tyr694) antibody (Cat No.: 12901042, Thermo Fisher, USA) and isotype control (Cat No.: 12471482, Thermo Fisher, USA), respectively. Finally, the cells were measured using a flow cytometer (BD FACSCalibur)

(16) Co-cultures of colon cancer cells and cancer-associated fibroblasts recapitulate the aggressive features of mesenchymal-like colon cancerFrontiers in ImmunologyMay 16, 2023Esther Strating, Mathijs P. Verhagen, Emerens Wensink, Ester Dünnebach, Liza Wijler, Itziar Aranguren, Alberto Sanchez De la Cruz, Niek A. Peters, Joris H. Hageman, Mirjam M. C. van der Net, et al.antibody (PE-conjugated, R&D Systems, FAB-3715P, 10µl/106 cells) or isotype control (Thermo Fisher, 12-4714-82, 5µl/106 cells). After 30 min incubation on ice, cells were washed two times and fluorescence was measured

(17) CD95/Fas ligand mRNA is toxic to cells through more than one mechanismMol BiomedApril 15, 2023Haluck-Kangas A, Fink M, Bartom ET, Peter ME.conjugated-anti-CD95 (eBioscience, cat #12–0951-83; RRID: AB_465789); PE conjugated Isotype control (eBioscience, cat #12–4714-82; RRID: AB_470060); anti-β-actin antibody (Santa Cruz, #sc-47778, RRID: AB_626632); anti-AGO2 (Abcam,

(18) Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM ProteinsFrontiers in cell and developmental biologyJanuary 1, 2022Gao Y, Tian Z, Liu Q, Wang T, Ban LK, Lee HH, Umezawa A, Almansour AI, Arumugam N, Kumar RS, et al."Thermo Fisher Scientific Inc. (Waltham, MA, United States)" "Mouse IgG1 kappa Isotype, PE",IgG1 isotype-PE,12-4714-82,"Thermo Fisher Scientific Inc. (Waltham, MA, United States)" Anti-nestin antibody,Nestin antibody,ab176571,"Abcam

(19) Cyclical depressurization degranulates platelets in an agonist-free mechanism of platelet activationPloS oneJanuary 1, 2022Velasquez-Mao AJ, Velasquez M, Vandsburger MH.subtract nonspecific binding (Fig 1D). Isotype controls for FITC (Thermo, 11-4714-81) and PE (Thermo, 12-4714-82) were used for each sample to subtract nonspecific binding. Single-plex fluorescence was measured on

(20) Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In VitroCellsDecember 10, 2021Basmaeil Y, Bahattab E, Al Subayyil A, Kulayb HB, Alrodayyan M, Abumaree M, Khatlani T.the data. The cells stained with PE-conjugated Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), cat # 12-4714-82, and/or FITC-conjugated Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), cat # 11-4714-81 (Thermo Fisher Scientific

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10/21/2019A Good Isotype Antibody for Assessing Non-Specific Antibody Binding on Peripheral CD14+ Monocytes by Flow Cytometry Chris Corkum,Recommended for use as an isotype control in flow cytometry experiments involving PBMC samples or purified CD14+ monocytes when properly matched to a test antibody.

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Mouse IgG1 kappa PE Isotype Control from Thermo Fisher Scientific

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Mouse IgG1 kappa PE Isotype Control from Thermo Fisher Scientific

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