CD4 Antibody APC-eFluor 780 47-0049-42 from Thermo Fisher Scientific

Product Specs
ItemCD4 Antibody APC-eFluor 780
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number47-0049-42
Quantity100 Tests
ApplicationsFlow Cytometry
Concentration5 µL/Test
Conjugate/TagAPC-eFluor™ 780
FormatPBS with BSA and 0.09% sodium azide; pH 7.2
ReactivityHuman
TargetCD4
IsotypeIgG1 kappa
CloneRPA-T4
HostMouse
NCBI Full Gene NameCD4 molecule
NCBI Gene AliasesCD4mut, IMD79, Leu-3, OKT4D, T4
ClonalityMonoclonal
PurityAffinity chromatography
Storage Temperature4° C, store in dark, DO NOT FREEZE!
Antibody TypePrimary
Storage4°C, store in dark, DO NOT FREEZE!

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) B and T cell responses to pre-erythrocytic R21/Matrix-M and blood-stage RH5.1/Matrix-M malaria vaccines in endemic settingsFront ImmunolMarch 2, 2026Caroline K. Bundi, Olivia Muñoz, Elizabeth Kibwana, Duncan Bellamy, Lisa Stockdale, Jordan R. Barrett, Kirsty McHugh, Ivanny Mtaka, Wilmina Kalinga, Domtila Kimani, et al.CD45RA-eF450 (304123, Biolegend), CXCR5-FITC (356913, Biolegend), CCR6-PE (G034E3, G034E3), CD4-APC-eF780 (47-0049-42, eBioscience), CD3-AF700 (56-0038-82, eBioscience) and CXCR3-APC (353708, Biolegend) at 37°C. Cells

(2) The Mitigating Effect of Combined Glucocorticoids with Immune Checkpoint Inhibitors on Lymphocyte Activation Gene-3 and Programmed Death-1 ExpressionEur J ImmunolAugust 11, 2025Smadar Gertel, Ari Polachek, Victoria Furer, Tali Ofir Dovrat, Chen Avaky, Adi Broyde, Hila Nochimovitz, Ori Elkayamat room temperature with the following anti‐human antibodies: APC‐eFluor780 anti‐CD4 (eBioscience, 47‐0049‐42), Pacific Blue anti‐CD8 (BLG‐301023, BioLegend), FITC‐anti‐CD14 (BLG‐325604), or PE‐anti‐CD14 (BLG‐301850),

(3) Single-cell transcriptional analysis reveals allergen-specific signatures in human γδ T cellsJCI InsightJune 9, 2025Kendall Kearns, Sloan A. Lewis, Esther Dawen Yu, Adam Abawi, Eric Wang, Synaida Maiche, Monalisa Mondal, Pandurangan Vijayanand, Grégory Seumois, Bjoern Peters, et al.anti-CD3 (AF700) (UCHT1, Life Technologies, 56-0038-42), anti-CD4 (APCef780) (RPA-T4, Life Technologies, 47-0049-42), anti-CD8 (PerCP-Cy5.5) (RPA-T8, Life Technologies, 45-0088-42), anti-CD14 (V500) (M5E2, BD Biosciences,

(4) Clinical and immunological outcomes after randomized trial of baked milk oral immunotherapy for milk allergyJCI InsightJanuary 9, 2025Jennifer A. Dantzer, Sloan A. Lewis, Kevin J. Psoter, Aaron Sutherland, April Frazier, Eve Richardson, Synaida Maiche, Gregory Seumois, Bjoern Peters, Robert A. Woodblocking TruStain FX (BioLegend, 422302), anti-CD4 (APCef780, clone RPA-T4, Thermo Fisher Scientific, 47-0049-42), anti-CD3 (Alexa Fluor 700, clone UCHT1, Thermo Fisher Scientific, 56-0038-42), anti-CD8/-CD14/-CD19/-CD56

(5) Ex vivo assays show human gamma-delta T cells specific for common allergens are Th1-polarized in allergic donorsCell Rep MethodsDecember 19, 2022Yu ED, Wang E, Garrigan E, Sutherland A, Khalil N, Kearns K, Pham J, Schulten V, Peters B, Frazier A, et al.

(6) A Novel Homozygous Stop Mutation in IL23R Causes Mendelian Susceptibility to Mycobacterial DiseaseJournal of clinical immunologyNovember 1, 2022Staels F, Lorenzetti F, De Keukeleere K, Willemsen M, Gerbaux M, Neumann J, Tousseyn T, Pasciuto E, De Munter P, Bossuyt X, et al.

(7) Transcriptomics of Acute DENV-Specific CD8+ T Cells Does Not Support Qualitative Differences as Drivers of Disease SeverityVaccines (Basel)April 14, 2022Alba Grifoni et al.780, clone RPA-T4 catalog #301042, eBioscience, San Diego, CA, USA) CD8 (BV650, clone RPA-T8 catalog #47-0049-42, Biolegend, San Diego, CA, USA), CD14 (V500, clone M5E2, catalog# 561391, BD Biosciences, San Diego,

(8) Epitope length variants balance protective immune responses and viral escape in HIV-1 infectionCell reportsMarch 1, 2022Pymm P, Tenzer S, Wee E, Weimershaus M, Burgevin A, Kollnberger S, Gerstoft J, Josephs TM, Ladell K, McLaren JE, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, anti-human-CD4–APC-eFluor780,eBioscience,Cat#47-0049-42 RRID:AB_1272044 anti-human-CD8–PerCP,BD Biosciences,Cat#347314 RRID:AB_400280 anti-human-CD28,BioLegend,Cat#

(9) IL4I1 binds to TMPRSS13 and competes with SARS-CoV-2 spikeFrontiers in immunologyJanuary 1, 2022Gatineau J, Nidercorne C, Dupont A, Puiffe ML, Cohen JL, Molinier-Frenkel V, Niedergang F, Castellano F.(11668027), eBioscience Fixable Viability Dye-efluor 450 (65-0863), anti-human CD4 APC-eFlour 780 (47-0049-42, clone RPA-T4), anti-human CD28 (16-0289-81, clone 28.2), and their respective isotype controls were

(10) Regulatory T cells promote cancer immune-escape through integrin αvβ8-mediated TGF-β activationNature communicationsOctober 28, 2021Lainé A, Labiad O, Hernandez-Vargas H, This S, Sanlaville A, Léon S, Dalle S, Sheppard D, Travis MA, Paidassi H, et al.

(11) Cell-density independent increased lymphocyte production and loss rates post-autologous HSCTeLifeFebruary 4, 2021Baliu-Piqué M, van Hoeven V, Drylewicz J, van der Wagen LE, Janssen A, Otto SA, van Zelm MC, de Boer RJ, Kuball J, Borghans JA, et al.UCHT1,‘(1:25)’ Antibody,"Anti-human CD4-APC-eF780 (Mouse IgG1, κ) RRID:AB_1272044",eBioscience,Cat# 47-0049-42 Clone: RPA-T4,‘(1:50)’ Antibody,"Anti-human CD8-V500 (Mouse IgG1, κ) RRID:AB_2870326",BD Biosciences,Cat#

(12) NKG2C^pos NK Cells Regulate the Expansion of Cytomegalovirus-Specific CD8 T CellsJournal of immunology (Baltimore, Md. : 1950)June 1, 2020Grutza R, Moskorz W, Senff T, Bäcker E, Lindemann M, Zimmermann A, Uhrberg M, Lang PA, Timm J, Cosmovici C.

(13) T Cell Responses Induced by Attenuated Flavivirus Vaccination Are Specific and Show Limited Cross-Reactivity with Other Flavivirus SpeciesJournal of virologyMay 4, 2020Grifoni A, Voic H, Dhanda SK, Kidd CK, Brien JD, Buus S, Stryhn A, Durbin AP, Whitehead S, Diehl SA, et al.

(14) Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue DiseaseCell reportsDecember 24, 2019Tian Y, Seumois G, De-Oliveira-Pinto LM, Mateus J, Herrera-de la Mata S, Kim C, Hinz D, Goonawardhana NDS, de Silva AD, Premawansa S, et al.Fluor 700",BioLegend,Cat#317340; Clone OKT3; RRID: AB_2563408 "CD4, APC eFluor 780",eBioscience,Cat#47–0049–42; Clone RPA-T4; RRID: AB_1272044 "CD8, Brilliant Violet 650",BioLegend,Cat#301042; Clone RPA-T8; RRID:

(15) Circulating T cell-monocyte complexes are markers of immune perturbationseLifeJune 25, 2019Burel JG, Pomaznoy M, Lindestam Arlehamn CS, Weiskopf D, da Silva Antunes R, Jung Y, Babor M, Schulten V, Seumois G, Greenbaum JA, et al.300415,1:200 (1 µl per test) Antibody,CD4-APCeF780; clone RPA-T4; mouse monoclonal,eBiosciences,Cat# 47-0049-42,1:200 (1 µl per test) Antibody,CD5-APCCy7; clone L17F12; mouse monoclonal,Biolegend,Cat# 364009,1:66

(16) RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody ResponsesCellOctober 4, 2018Bradley T, Peppa D, Pedroza-Pacheco I, Li D, Cain DW, Henao R, Venkat V, Hora B, Chen Y, Vandergrift NA, et al.AB_2132983 "CD4 Monoclonal Antibody, APC-eFluor 780, Clone RPA-T4","eBioscience, Thermo Fisher Scientific",Cat# 47-0049-42; RRID: AB_1272044 "CD8a Monoclonal Antibody, Alexa Fluor 700, Clone OKT8","eBioscience, Thermo Fisher Scientific

(17) Activation-induced Markers Detect Vaccine-Specific CD4⁺ T Cell Responses Not Measured by Assays Conventionally Used in Clinical TrialsVaccinesJuly 31, 2018Bowyer G, Rampling T, Powlson J, Morter R, Wright D, Hill AVS, Ewer KJ.

(18) CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancerJCI insightJune 2, 2017Gu-Trantien C, Migliori E, Buisseret L, de Wind A, Brohée S, Garaud S, Noël G, Dang Chi VL, Lodewyckx JN, Naveaux C, et al.

(19) Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancerCell researchApril 1, 2017Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, et al.

(20) Protein kinase CK2 controls T-cell polarization through dendritic cell activation in response to contact sensitizersJournal of leukocyte biologyMarch 1, 2017de Bourayne M, Gallais Y, El Ali Z, Rousseau P, Damiens MH, Cochet C, Filhol O, Chollet-Martin S, Pallardy M, Kerdine-Römer S.

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CD4 Antibody APC-eFluor 780 from Thermo Fisher Scientific

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CD4 Antibody APC-eFluor 780 from Thermo Fisher Scientific

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