Association between PD-1, Tim-3 or Lag-3 expression on CD8 T cells with T-bet/Eomes and CD39 expression.The strategy for gating CD8 T cells based on T-bet and Eomes expression is shown in (a). Expression of PD-1 (b), Tim-3 (c), Lag-3 (d), CD38 (e) and CD39 (f) was compared between CD8 T cells staining T-bethi/Eomesdim and T-betdim/Eomeshi populations. The panels on the right show the expression of these markers from a single, representative sample with T-bethi/Eomesdim (red line) and T-betdim/Eomeshi (blue line) overlaid with gating control (orange line). The shaded area highlights cells that were considered positive for the marker. Expression of exhaustion markers between two subsets was compared using Wilcoxon matched-pairs signed rank test.