Mouse IgG2b kappa PE Isotype Control 12-4732-81 from Thermo Fisher Scientific

Product Specs
ItemMouse IgG2b kappa PE Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number12-4732-81
PurityAffinity chromatography
Antibody TypeIsotype Control
ClonalityMonoclonal
Storage4°C, store in dark, DO NOT FREEZE!
Storage Temperature4° C, store in dark, DO NOT FREEZE!
ApplicationsFlow Cytometry (FCM)
HostMouse
CloneeBMG2b
IsotypeIgG2b kappa
TargetMouse IgG2b kappa
FormatPBS with 0.09% sodium azide; pH 7.2
Conjugate/TagPE
Concentration0.2 mg/mL
Quantity50 µg

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) DSG2-Directed CAR-T Cells Safely and Universally Eliminate Solid TumorsResearch SquareFebruary 12, 2026Adam Snook, Robert Carlson, Lindsay Weil, Trevor Baybutt, Ozlem Kulak, Miao Cao, Ross Staudt, Pranav Jain, Madison Crutcher, Ariana Entezari, et al.determined by comparison to identical cell lines stained with IgG2bκ-PE isotype control (ThermoFisher, #12-4732-81). Quantitative comparisons were made with BD QuantiBRITE-PE beads ran in unison as a pre-calibrated

(2) The PTBP1-EZH2-DOCK2 axis promotes M1 microglial polarization and exacerbates neuronal apoptosis following spinal cord injuryJournal of Orthopaedic TranslationJanuary 22, 2026Chunhe Sha, Feng Pan, Xiaodong Liu, Zhiqing Wang, Hongbin Dai, Kai HuangPerCP-Cy5.5 Mouse IgG1 kappa (Clone MOPC-21; 550795, BD Biosciences), PE Mouse IgG2b kappa (Clone eBMG2b; 12-4732-81, Thermo Fisher Scientific), and PE Mouse IgG1 kappa (Clone P3.6.2.8.1; 12-4714-82, Thermo Fisher Scientific).

(3) Determining the Importance of Carbohydrate-Based Structures in Murine Norovirus Binding to Commensal BacteriaVirusesAugust 20, 2025Jasmine L. Madrigal, Joseph P. Sullivan, Feba Mathew, Melanie Lane, Melissa K. Jonesconjugated NoV-antibody dilution, the same dilution of isotype control (IgG2b, Invitrogen, Waltham, MA, USA; 12473281) or blocking buffer (negative control). Samples were placed on ice and incubated in the dark for 30

(4) The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer developmentJournal for immunotherapy of cancerMay 1, 2023Zhang C, Wei S, Dai S, Li X, Wang H, Zhang H, Sun G, Shan B, Zhao L.FITC-conjugated anti-CD206 (MA5-16870), PE-conjugated anti-CD163 (12-1639-42), Mouse IgG2b κ Isotype Control PE (12-4732-81), Mouse IgG1, κ Isotype Control Alexa Fluor 488 (53–4714) (all the antibodies purchased from eBioscience,

(5) Baicalin Modulates Inflammatory Response of Macrophages Activated by LPS via Calcium-CHOP PathwayCellsSeptember 30, 2022An HJ, Lee JY, Park W.mouse IgG1 kappa isotype control (eBioscience 12-4714-81), and mouse IgG2b kappa isotype control (eBioscience 12-4732-81). After washing the cells with staining buffer, the levels of phosphorylated P38 MAPK and Fas were analyzed

(6) Berberine modulates hyper-inflammation in mouse macrophages stimulated with polyinosinic-polycytidylic acid via calcium-CHOP/STAT pathwayScientific reportsMay 28, 2021Kim HJ, Kim YJ, Park W.Mouse IgG1 kappa Isotype Control (eBioscience 12-4714-81), and Mouse IgG2b kappa Isotype Control (eBioscience 12-4732-81) from Life Technologies Corporation (Carlsbad, CA, USA). All other solutions for flow cytometric analysis

(7) Rubi Fructus Water Extract Alleviates LPS-Stimulated Macrophage Activation via an ER Stress-Induced Calcium/CHOP Signaling PathwayNutrientsNovember 22, 2020Kim DH, Lee JY, Kim YJ, Kim HJ, Park W.(T180/Y182) (eBioscience 17-9078-42) and Mouse immunoglobulin G2b (IgG2b) kappa Isotype Control (eBioscience 12-4732-81) were obtained from Life Technologies Corporation (Carlsbad, CA, USA). 2.2. Preparation of RF Commercial

(8) Diospyrin Modulates Inflammation in Poly I:C-Induced Macrophages via ER Stress-Induced Calcium-CHOP PathwayProcessesAugust 27, 2020Hyun-Ju Kim, Inamullah Khan, Adnan Shahidullah, Syed Muhammad Ashhad Halimi, Abdur Rauf, Ji-Young Lee, Young-Jin Kim, Bong-Youn Kim, Wansu Park12‐4714‐81), and Mouse IgG2b kappa Isotype stress-induced calcium-CHOP pathway. Control (eBioscience 12‐4732‐81) were from Life Technologies Corporation (Carlsbad, CA, USA). 2. Materials and Methods 2.2. Cell Culture 2.1.

(9) Antitumor immunity targeting fibroblast activation protein-α in a mouse Lewis lung carcinoma modelOncology lettersJuly 1, 2020Xie J, Yuan S, Peng L, Li H, Niu L, Xu H, Guo X, Yang M, Duan F.isotype-matched antibodies; IgG Isotype Control (cat. no. 12-4888-81), IgG2b kappa Isotype Control (cat. no. 12-4732-81) and IgG2b kappa Isotype Control (cat. no. 12-4031-80) in the dark for 30 min at 4°C (all 1:20 and from

(10) CD279 mediates the homeostasis and survival of regulatory T cells by enhancing T cell and macrophage interactionsFEBS open bioJune 1, 2020Liu Y, Gao Y, Hao H, Hou T.

(11) External Beam Radiation Therapy and Enadenotucirev: Inhibition of the DDR and Mechanisms of Radiation-Mediated Virus IncreaseCancersMarch 26, 2020Pokrovska TD, Jacobus EJ, Puliyadi R, Prevo R, Frost S, Dyer A, Baugh R, Rodriguez-Berriguete G, Fisher K, Granata G, et al.of 1:20 (#12-9159-42, Invitrogen), or appropriate isotype control (#400114, BioLegend for CD46 and #12-4732-81, Invitrogen for DSG2), was performed in MACS buffer (0.5% BSA, 2 mM EDTA in PBS). Data was acquired

(12) Anti-Inflammatory Effects of Diospyrin on Lipopolysaccharide-Induced Inflammation Using RAW 264.7 Mouse MacrophagesBiomedicinesJanuary 11, 2020Shahidullah A, Lee JY, Kim YJ, Halimi SMA, Rauf A, Kim HJ, Kim BY, Park W.MAPK Antibody (T180/Y182) (eBioscience 17-9078-42) and Mouse IgG2b kappa Isotype Control (eBioscience 12-4732-81) 2.3. Quantification of NO were obtained from Life Production Technologies Corporation (Carlsbad, CA,

(13) Salvianolic acid B reverses multidrug resistance in nude mice bearing human colon cancer stem cellsMolecular medicine reportsAugust 1, 2018Guo P, Wang J, Gao W, Liu X, Wu S, Wan B, Xu L, Li Y.eBioscience; Thermo Fisher Scientific, Inc.) and mouse IgG2b kappa isotype control (1:100; cat. no. 12-4732-81; eBioscience; Thermo Fisher Scientific, Inc.). Subsequently, the cells were washed twice with PBS and

(14) ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting PhosphatasesFrontiers in immunologyJanuary 1, 2018Glal D, Sudhakar JN, Lu HH, Liu MC, Chiang HY, Liu YC, Cheng CF, Shui JW.eBiosciense™ (clone eBMG2b) (1:100), Isotype control for pSTAT3",Flow cytometry,ThermoFisher Scientific (12-4732-81) "PE/Cy7 anti-mouse/human CD44, clone IM7",Flow cytometry,BioLegend (103029) "APC anti-mouse CD3 Antibody,

(15) Platelets confound the measurement of extracellular miRNA in archived plasmaScientific reportsSeptember 13, 2016Mitchell AJ, Gray WD, Hayek SS, Ko YA, Thomas S, Rooney K, Awad M, Roback JD, Quyyumi A, Searles CD.

(16) Effect of cold stress on immunity in ratsExperimental and therapeutic medicineJanuary 1, 2016Hu GZ, Yang SJ, Hu WX, Wen Z, He D, Zeng LF, Xiang Q, Wu XM, Zhou WY, Zhu QX.

(17) Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cellsNucleic acids researchFebruary 18, 2015Byrne SM, Ortiz L, Mali P, Aach J, Church GM.

(18) PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1BloodJanuary 9, 2014Wu J, Zhou LQ, Yu W, Zhao ZG, Xie XM, Wang WT, Xiong J, Li M, Xue Z, Wang X, et al.

(19) A novel llama antibody targeting Fn14 exhibits anti-metastatic activity in vivomAbsJanuary 1, 2014Trebing J, Lang I, Chopra M, Salzmann S, Moshir M, Silence K, Riedel SS, Siegmund D, Beilhack A, Otto C, et al.

(20) Soluble rank ligand produced by myeloma cells causes generalised bone loss in multiple myelomaPloS oneJanuary 1, 2012Buckle CH, De Leenheer E, Lawson MA, Yong K, Rabin N, Perry M, Vanderkerken K, Croucher PI.

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Mouse IgG2b kappa PE Isotype Control from Thermo Fisher Scientific

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Mouse IgG2b kappa PE Isotype Control from Thermo Fisher Scientific

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